Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid.

Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid.

The present disclosure provides, in some aspects, methods and compositions for producing nucleic acid nanostructures having little to no kinetic barriers to self-assembly.

The present disclosure provides, in some aspects, methods and compositions for producing nucleic acid nanostructures having little to no kinetic barriers to self-assembly.

A porous matrix according to the present disclosure, wherein a nucleic acid primer-carbon material composite in which one or more nucleic acid primer of a forward primer and a reverse primer as a polymerase chain reaction (PCR) primer is bound to a carbon material is included in the pores of the matrix, provides improved amplification efficiency as compared to a matrix wherein the nucleic acid primer is present on the outer surface of the matrix or a porous matrix wherein the nucleic acid primer is directly fixed inside pores. The porous matrix of the present disclosure can effectively detect various kinds of target nucleic acids simultaneously and analyze them in real time by varying the kinds of the nucleic acid primers included in the matrix. Therefore, it is useful in amplifying multiple nucleic acids.

A porous matrix according to the present disclosure, wherein a nucleic acid primer-carbon material composite in which one or more nucleic acid primer of a forward primer and a reverse primer as a polymerase chain reaction (PCR) primer is bound to a carbon material is included in the pores of the matrix, provides improved amplification efficiency as compared to a matrix wherein the nucleic acid primer is present on the outer surface of the matrix or a porous matrix wherein the nucleic acid primer is directly fixed inside pores. The porous matrix of the present disclosure can effectively detect various kinds of target nucleic acids simultaneously and analyze them in real time by varying the kinds of the nucleic acid primers included in the matrix. Therefore, it is useful in amplifying multiple nucleic acids.

An apparatus for nucleic acid sequencing includes a nanochannel and a conveying device, configured to move a nucleic acid strand through the nanochannel. The conveying device includes: a first electrode, a second electrode, and a third electrode, which are arranged along the nanochannel so as to be in contact with a fluid occupying the nanochannel, the second electrode being arranged between the first electrode and the third electrode; and a control unit configured to apply a first voltage, a second voltage, and a third voltage, respectively, to the first electrode, the second electrode and the third electrode, for controlling movement of the nucleic acid strand through the nanochannel.

Methods, devices, and/or systems for providing carbon nanotube material that interacts with nucleotides to form CNT-nucleotide nanostructures wherein the CNT-nucleotide nanostructures form detectable network structures upon reactions with nucleic acids having targeted sequences.

Methods, devices, and/or systems for providing carbon nanotube material that interacts with nucleotides to form CNT-nucleotide nanostructures wherein the CNT-nucleotide nanostructures form detectable network structures upon reactions with nucleic acids having targeted sequences.

The invention relates to a new method of delivering an analyte to a transmembrane pore in a membrane. The method involves the use of microparticles.