Single-cell multi-omics by co-encapsulating a single cell with two beads, the first an RNA barcoding bead having barcoded mRNA capture primer oligonucleotides attached on the bead surface; and the second a DNA barcoding bead having two types of oligonucleotides releasably attached to the surface: (1) barcoded adapter oligonucleotides that are complementary to oligonucleotides bound to the transposase that are eventually incorporated into gDNA fragments and (2) polyadenylated barcoded oligonucleotides containing the same barcode sequence as the adapters. In addition, integrated analysis of RNA and protein, including intracellular protein, from individual cells using similar co-encapsulation of a single cell, an RNA barcoding bead, and with/without a specific or non-specific protein binding bead in a microwell, to avoid protein fixation by first lysing the cell to liberate intracellular contents, and then capturing protein either on a solid surface or in solution with barcoded affinity reagents.

Single-cell multi-omics by co-encapsulating a single cell with two beads, the first an RNA barcoding bead having barcoded mRNA capture primer oligonucleotides attached on the bead surface; and the second a DNA barcoding bead having two types of oligonucleotides releasably attached to the surface: (1) barcoded adapter oligonucleotides that are complementary to oligonucleotides bound to the transposase that are eventually incorporated into gDNA fragments and (2) polyadenylated barcoded oligonucleotides containing the same barcode sequence as the adapters. In addition, integrated analysis of RNA and protein, including intracellular protein, from individual cells using similar co-encapsulation of a single cell, an RNA barcoding bead, and with/without a specific or non-specific protein binding bead in a microwell, to avoid protein fixation by first lysing the cell to liberate intracellular contents, and then capturing protein either on a solid surface or in solution with barcoded affinity reagents.

Methods and apparatuses for performing Free-Running Synthesis (FRS) and library preparation steps (e.g., nanopore library preparation) on a cartridge using digital microfluidics (DMF) in a tabletop DMF driver/reader apparatus.

Methods and apparatuses for performing Free-Running Synthesis (FRS) and library preparation steps (e.g., nanopore library preparation) on a cartridge using digital microfluidics (DMF) in a tabletop DMF driver/reader apparatus.

Provided herein are methods for enrichment of nucleic acid libraries for non-artefactual on-target molecules that produce bona fide sequencing reads while eliminating or reducing artefactual on-target molecules that produce wasted reads thereby improving sequencing efficiency. Methods comprise the neutralization of a single artefactual strand of a dsDNA molecule and capture of the non-artefactual strand thereby generating enriched sequencing libraries.

Provided herein are methods for enrichment of nucleic acid libraries for non-artefactual on-target molecules that produce bona fide sequencing reads while eliminating or reducing artefactual on-target molecules that produce wasted reads thereby improving sequencing efficiency. Methods comprise the neutralization of a single artefactual strand of a dsDNA molecule and capture of the non-artefactual strand thereby generating enriched sequencing libraries.

The present invention provides methods, compositions, and systems for distributing molecules and complexes into reaction sites. In particular, the methods, compositions, and systems of the present invention result in an active loading of molecules and complexes into reaction sites with improved efficiency over loading by passive diffusion methods alone.

The present invention provides methods, compositions, and systems for distributing molecules and complexes into reaction sites. In particular, the methods, compositions, and systems of the present invention result in an active loading of molecules and complexes into reaction sites with improved efficiency over loading by passive diffusion methods alone.

The invention relates to new methods for synthesising polynucleotide molecules according to a predefined nucleotide sequence. The invention also relates to methods for the assembly of synthetic polynucleotides following synthesis, as well as systems and kits for performing the synthesis and/or assembly methods.

The invention relates to new methods for synthesising polynucleotide molecules according to a predefined nucleotide sequence. The invention also relates to methods for the assembly of synthetic polynucleotides following synthesis, as well as systems and kits for performing the synthesis and/or assembly methods.