The disclosure relates to insecticidal toxin resistance management and screening of novel insecticidal toxin receptors for insect resistance. One embodiment relates to the isolation, characterization, compositions, and methods of use relating to polynucleotides encoding novel insecticidal toxin receptors and the polypeptides encoded thereby. The polynucleotides and polypeptides are useful in identifying or designing insect pest management strategies.

The present invention provides methods for predicting the pattern of locomotion in a horse including the ability of a horse to use different gaits and the ability to trot at a fast speed. The methods comprise determining in a sample of DNA obtained from a horse the presence or absence of at least one genetic marker, wherein said at least one genetic marker is located on horse chromosome 23, said marker being associated with the ability to use different gaits. The invention further provides primers that amplify markers being associated with the ability to use different gaits and hybridization probes to detect markers being associated with the ability to use different gaits and the ability to trot at a fast speed.

The present disclosure discloses a molecular marker for identifying poultry laying traits based on an OVR gene, an identification method and an application thereof. The molecular marker is a polymorphic site which is developed on the basis of an oocyte vitellogenesis receptor (OVR) gene and capable of affecting the expression quantity thereof. The expression of the OVR gene is affected by the binding efficiency of C/EBPα to the transcriptional control region. The molecular marker is a G/T mutation located at −399 bp upstream of the transcription initiation site of the OVR gene; the type of the molecular marker is identified in the poultry genome to judge the egg laying capacity of an individual poultry via molecular screening at the early stage, which provides direct technical means for the breeding of poultry egg laying capacity.

Methods of identifying a dog as having deficient nucleic base salvage and methods of identifying a dog as being at an elevated risk and increased likelihood of developing immune dysfunction are disclosed. Methods of treating a dog to prevent, delay onset of or reduce severity of symptoms of immune dysfunction in the dog and methods of treating a dog diagnosed with or suspected of having immune dysfunction are disclosed. Methods of treating a dog diagnosed with or suspected of having immune dysfunction are disclosed. The disclosed methods include performing a genetic analysis of the dog to detect the presence of 2 copies of major allele G for single nucleotide polymorphism Affx-205876096. Nutritionally complete and balanced dog food compositions are disclosed. The nutritionally complete and balanced dog food compositions comprise a therapeutic level of nucleotides.

The invention relates in general to the analysis of genetic markers in individuals of non-human origin, in particular the genotyping of outbred populations of rodents or fish. The invention relates specifically to the simultaneous amplification of at least five different polymorphic autosomal markers and at least one polymorphic Y-chromosomal marker of the mouse in a reaction preparation by means of polymerase chain reaction or other multiplex methods and the detection of the specific alleles for each marker of the multiplex method. The invention further relates to a kit for genetically identifying and/or for differentiating two or more animals from DNA extracts of individuals from wild-type populations, from different inbred or outbred strains, of an identical inbred or outbred strain, or from substrains of an identical inbred or outbred strain, in particular of the rat or mouse.

Differentially expressed miRNA or small mRNA in the culture media of embryos were found to correlate to and affect embryo developmental fate. Accordingly the present invention provides a method for selecting a bovine embryo for implantation into a female bovine animal for further development based on the levels of specific miRNA or small mRNA. Also provided are methods of improving bovine embryo development fate by reducing in the culture medium the level of selected miRNAs.

The present invention provides methods for predicting the pattern of locomotion in a horse including the ability of a horse to use different gaits and the ability to trot at a fast speed. The methods comprise determining in a sample of DNA obtained from a horse the presence or absence of at least one genetic marker, wherein said at least one genetic marker is located on horse chromosome 23, said marker being associated with the ability to use different gaits. The invention further provides primers that amplify markers being associated with the ability to use different gaits and hybridization probes to detect markers being associated with the ability to use different gaits and the ability to trot at a fast speed.

Methods are provided for determining whether a subject has a graft tolerant phenotype. In practicing the subject methods, the expression of at least 5 genes in a sample from the subject, e.g., a blood sample, is assayed to obtain a gene expression result for the at least 5 genes. The obtained gene expression result for the at least 5 genes is then employed to determine whether the subject has a graft tolerant phenotype. Also provided are compositions, systems and kits that find use in practicing the subject methods. The methods and compositions find use in a variety of applications, including the determination of an immunosuppressive therapy regimen.

The present invention provides screening kits, compositions, and diagnostic methods for determining whether a subject has a predisposition to, or likelihood of having, a substance use disorder by determining a nucleic acid methylation profile from a biological sample from the subject, wherein a given profile indicates that the subject has a predisposition to a substance use disorder.

Single nucleotide polymorphic sites of the bovine MAP1B, PPP1R11, and DDX4 genes are associated with improved bull fertility as measured by e.g. sire conception rates. Nucleic acid molecules, arrays, kits, methods of genotyping and marker-assisted bovine breeding methods based on these SNPs are disclosed.