The present invention discloses a SNP marker combination and an identification method for Pudong white pigs and raw meat products thereof, including: extracting genomic DNAs of raw pork or meat products, performing agarose gel electrophoresis and Sanger sequencing after PCR amplification, and identifying Pudong white pigs and meat products thereof according to SNP genotypes of characteristic loci of sequencing results; as for the Sanger sequencing, its identification loci are as follows: specific mutations occur at loci of pig18-52722267, pig8-146130825, pig9-10041850 and pig13-213464983. The present invention solves the problem that there is no identification method related to Pudong white pigs and meat products thereof in the prior art.

The present disclosure relates methods and compositions useful for prevention of porcine reproductive and respiratory syndrome virus (PRRSv) in animals, including animals of the species Sus scrofa. The present teachings relate to swine wherein at least one allele of a CD163 gene has been inactivated, and to specific methods and nucleic acid sequences used in gene editing to inactivate the CD163 gene. Swine wherein both alleles of the CD163 gene are inactivated are resistant to porcine reproductive and respiratory syndrome virus (PRRSv). Elite lines comprising homozygous CD163 edited genes retain their superior properties.

Provided are an egg-type chicken whole-genome SNP chip and the use thereof. The SNP loci of the chip are respectively derived from 14,624 SNP loci shared by each line of the major egg-type chickens in China; 3,677 SNP loci associated with disease-resistant traits in egg-type chickens; 16,000 SNP loci associated with economic traits in egg-type chickens; and 9,358 SNP loci making up genomic regions not covered by the foregoing probes. The 43,681 SNPs on the egg-type chicken whole-genome SNP chip have DNA sequences as represented by SEQ ID NO. 1-43,681. The chip can specifically identify the genetic relationship between commercial egg-type chickens and egg-type chickens of local breeds, and can also perform applications such as whole-genome association analysis, genome selective breeding, and QTL location analysis of target traits and population genetic analysis.

The disclosure relates to Bt toxin resistance management. One embodiment relates to the isolation and characterization of polynucleotides and polypeptides corresponding to novel Bt toxin receptors. The polynucleotides and polypeptides are useful in identifying or designing novel Bt toxin receptor ligands including novel insecticidal toxins.

The present invention relates to the determination of ages. Specifically, the present invention relates to a method for determining an age indicator, and a method for determining the age of an individual. Said methods are based on data comprising the DNA methylation levels of a set of genomic DNA sequences. Preferably, said age indicator is determined by applying on the data a regression method comprising a Least Absolute Shrinkage and Selection Operator (LASSO), preferably in combination with subsequent stepwise regression. Furthermore, the invention relates to an ensemble of genomic DNA sequences and a gene set, and their uses for diagnosing the health state and/or the fitness state of an individual and identifying a molecule which affects ageing. In further aspects, the invention relates to a chip or a kit, in particular which can be used for detecting the DNA methylation levels of said ensemble of genomic DNA sequences.

There is disclosed a method to predict the predisposition to an exercise performance trait in a human individual comprising: a) assaying a genetic sample obtained from the human individual for the presence of at least one genetic variation in each of the following genes: ACTN3, COL5A1, MCT1, VEGF, HFE, to obtain a polymorphism profile; b) determining a regression function associated to the polymorphism profile; c) classifying the individual's predisposition to the exercise performance trait on the basis of the value of said regression function.

The present invention relates to methods for diagnosing heart disease in a canine, including early stage degenerative mitral valve disease, by using microbiome including specific genera and species. In one embodiment, the method can comprise measuring a normalized relative abundance of fecal bacteria including Faecalibacterium, Turicibacter, Streptococcus, E. Coli, Blautia, Fusobacterium, and C. hiranonis, calculating a dysbiosis index based on the fecal bacteria, and determining that the canine has heart disease if the dysbiosis index is greater than −1.0.

The present disclosure relates to the technical field of molecular marker breeding of rabbits, in particular to a molecular marker related to a wool yield of a long-haired rabbit and use thereof in breeding. The molecular marker includes a mutant of a keratin 26 gene (KRT26 gene), where the KRT26 gene is as shown in SEQ ID NO: 1, the mutant of the KRT26 gene is formed by mutation of a base G at position 41844263 of a KRT26 gene locus to a base A. In the present disclosure, the wool yield trait of the long-haired rabbit and the KRT26 gene are subjected to association study and population verification, and it is found that an individual long-haired rabbit with an allele G has a higher wool yield, with an additive gene effect of 15.59 g; the base substitution can control an overall genetic variation of the wool yield by 1.51%.

The present disclosure relates to a set of molecular markers. The molecular markers include at least one selected from the group consisting of a mutant of a frizzled class receptor 3 gene (FZD3 gene) and a mutant of a keratin 26 gene (KRT26 gene), where the FZD3 gene is as shown in SEQ ID NO: 1, and the KRT26 gene is as shown in SEQ ID NO: 2; the mutant of the FZD3 gene is formed by mutation of a base T at position 41019916 of a FZD3 gene locus to a base C, and the mutant of the KRT26 gene is formed by mutation of a base G at position 41842284 of a KRT26 gene locus to a base A and/or formed by mutation of a base G at position 41842481 of the KRT26 gene locus to a base C.

The present disclosure relates methods and compositions useful for prevention of porcine reproductive and respiratory syndrome virus (PRRSv) in animals, including animals of the species Sus scrofa. The present teachings relate to swine wherein at least one allele of a CD163 gene has been inactivated, and to specific methods and nucleic acid sequences used in gene editing to inactivate the CD163 gene. Swine wherein both alleles of the CD163 gene are inactivated are resistant to porcine reproductive and respiratory syndrome virus (PRRSv). Elite lines comprising homozygous CD163 edited genes retain their superior properties