The invention belongs to the technical field of genetic engineering, and particularly to a type I-F CRISPR-Cas system based on Zymomonas mobilis (Z. mobilis) including: four CRISPR structural sequences and one cas gene cluster, wherein the cas gene cluster comprises casi gene, cas3 gene, csyl gene, csy2 gene, csy3 gene and csy4 gene, wherein the cast-3 gene is a fusion form fused by a cast gene and a cas3 gene. The purpose of the present invention is to use Z. mobilis as a model bacterium, using a CRISPR-Cas system encoded by the genome of the Z. mobilis and exogenous CRISPR-Cas12a system to build a genome editing platform so as to provide a set of powerful tools for carrying out basic and applied research in this bacterium and similar cells, and promoting the development of metabolic engineering, systems biology and synthetic biology.

A phosphorus-dissolving bacterium RBC25 regulated by Phosphate deficiency and application thereof are provided. The phosphorus-dissolving bacterium RBC25 is obtained by isolating from roots of soybean planted in acidic soil. The phosphorus-dissolving bacterium RBC25 belongs to Burkholderia sp., which has a dissolving capacity for insoluble inorganic phosphate and organic phosphate. The phosphorus-dissolving bacterium RBC25 is regulated by phosphate deficiency, which can effectively colonize on soybean roots under phosphate-deficient conditions, and thus promote soybean growth under low-phosphate conditions. The phosphorus-dissolving bacterium RBC25 releases dissolved phosphate by dissolving insoluble inorganic phosphate and organic phosphate, so that it helps plants to absorb insoluble phosphate. Compared with the non-inoculated control, inoculating RBC25 in greenhouses can increase the biomass and phosphorus content of soybean by 39.6% and 35.4%, respectively, and inoculating RBC25 in fields can increase the biomass and phosphorus content of soybean by 12.5% and 16.9%, respectively.

The present invention relates to a method for promoting growth of gas-fermented microorganisms, comprising the following steps: S1. ultrasonically blending a surfactant and a culture medium, then adding a fluorine-containing alkyl compound to a mixture, and ultrasonically processing the mixture to obtain a perfluorocarbon nanoemulsion; S2. inoculating a bacterial suspension into the perfluorocarbon nanoemulsion, and introducing simultaneously a mixed gas to obtain a precursor; S3. cultivating the precursor in a shaker, wherein the precursor has a diameter of bubbles of 2.0-4.2 mm, and the bubbles have the total volume of less than 40 ml. Under these conditions, the microorganisms have a high utilization rate of the gas, can grow and metabolize more quickly, and can obtain bacteria and products more quickly. Compared with a traditional culture system, addition of a mass transfer material can promote gas mass transfer. Therefore, the gas consumption is small, and the cost is low.

The present invention pertains to hybrid RNase H2 proteins that include fragments of amino acid sequences from Pyrococcus abyssi (P.a.), Thermococcus kodakarensis (T.kod), and Pyrococcus furiosus organisms, as well as methods of using the same to improve mismatch discrimination and activity in a high-fidelity DNA polymerase buffer.

The purpose of the present invention is to provide microorganisms which efficiently produce nicotinamide riboside, and microorganisms which can efficiently produce both nicotinamide mononucleotide and nicotinamide riboside. Nicotinamide mononucleotide and nicotinamide riboside can be produced by culturing lactic acid bacteria belonging to the genus Fructobacillus.

An objective of the present invention is to provide a production method that enables PHA (e.g. PHA powder) to be produced with high productivity. The above is achieved by providing: a method for producing a polyhydroxyalkanoate, including the steps of (a) preparing an aqueous suspension that contains a polyhydroxyalkanoate and an alkylene oxide-based dispersing agent and has a pH of not more than 7 and (b) spray-drying the aqueous suspension prepared in the step (a); and the like.

Surfactants based on a newly discovered class of compounds include a hydrophobic lipid oligomer covalently linked to a peptide or peptide-like chain and a carbohydrate moiety, and a serine-leucinol dipeptide linked to the lipid oligomer. Such surfactants can be used to create an oil-in-water or water-in-oil emulsion by mixing together a polar component; a non-polar component; and the surfactant. Biosurfactants of the newly discovered class can be made by isolating and culturing a microorganism which produces the biosurfactant, and then isolating the biosurfactant from the culture. A microorganism can be engineered to produce biosurfactant of this newly discovered class by expressing a set of heterologous genes involved in the biosynthesis of the biosurfactant in the microorganism.

The present disclosure provides a method of preparing a carbonaceous material capable of fixing arsenic and an application thereof. Through biomass pretreatment, biomass pyrolysis and arsenite fixation, a biochar activated by potassium carbonate and an arsenic-containing wastewater containing sulfur-containing substances are mixed and deoxidized, and an anaerobic culture is carried out, to fix arsenic by the biochar activated by potassium carbonate. The present disclosure solves the problems that arsenic is released from the soil and groundwater under anaerobic conditions, the ability of conventional passivatingagents to fix arsenic under anaerobic conditions is weakened, and the conventional carbonaceous materials not only cannot fix arsenic, but also accelerate the release of anaerobic arsenic.

This invention relates to novel probiotic Bifidobacteria strains, particularly, B. pseudocatenulatum strains, and their use as probiotic, and food products, feed products, dietary supplements and pharmaceutical formulations containing them. The bacteria are suitable for the treatment of obesity, diabetes, and related conditions.

U biosensors, and related U-sensing genetic molecular components, genetic circuits, compositions, methods and systems are described, which in several embodiments can be used to detect and/or neutralize uranium and in particular bioavailable U.