A method of producing succinic acid from a biomass. The method comprises adding citric acid to a biomass comprising microalgae to form an acidified microalgae composition. The acidified microalgae biomass composition is stored under anaerobic conditions without inoculating the acidified microalgae composition with bacteria formulated to produce succinic acid. A coproduct comprising succinic acid is produced. Other methods of producing succinic acid from a biomass are also disclosed.

Disclosed herein are well treatment composition and methods. In a specific embodiment, a well treatment composition includes: denatured algae, where the denatured algae have a protein content from about 20.0 wt % to about 90.0 wt %, and where the denatured algae have a lipid content from about 0.1 wt % to about 30.0 wt %; one or more carrier fluids; and one or more additives. In another specific embodiment, a method of making a well treatment composition can include contacting one or more algae with one or more denaturants to make a denatured algae, where the denatured algae have a protein content from about 20.0 wt % to about 90.0 wt %, and where the denatured algae have a lipid content from about 0.1 wt % to about 30.0 wt %; and contacting the denatured algae with one or more carrier fluids to make a well treatment composition.

A novel alga mutant having improved pigment productivity is provided. The mutant can be used to produce carotenoid-based pigments, particularly, xanthophyll by using a small amount of energy, and thus can effectively produce pigment on an industrial level. In addition, the mutant can be used as a source for foods, health functional foods, and medications which contain a pigment. Further, when account is taken of the physiological characteristics of the euryhaline microalgae Dunaliella and the geological characteristics of Korean, the three sides of which are in contact with the sea, sea-water can be used as a culture medium, with the expectation of reducing the cost and leading to the development of related industries.

Provided herein are methods for obtaining purified recombinant osteopontin (rOPN) from cultures of transgenic microalgae, as well as nutritional supplements from such cultures.

The present disclosure provides a resource-circulation-type and eco-friendly livestock manure treatment method, and a system for producing algal biomass used therein, the method being capable of: preventing water pollution while allowing livestock liquid fertilizer to be treated on the basis of a biological process; producing algal biomass; and supplying the produced algal biomass to livestock feed and farms.

The invention provides a recombinant algal organism that has been genetically modified in a gene encoding a protein kinase-like protein. The recombinant organism exhibits higher biomass productivity and higher lipid productivity versus a corresponding control algal organism not having the genetic modification. The recombinant organism is therefore useful in applications requiring biomass and/or lipid productivity, e.g. in the production of biofuels or other lipidic matter. Methods of using the organism and biomass containing or produced by the organism are also provided.

The present invention is directed to isolated thraustochytrid microorganisms as well as strains and mutants thereof. The invention is further directed to biomasses, microbial oils, compositions, cultures, methods of producing microbial oils, and methods of using the isolated thraustochytrids, biomasses, and microbial oils.

In ethyl alcohol production using the self-fermentation of a microalga, a step of concentrating or collecting an algal body by centrifugal treatment, filtering treatment or the like is made unnecessary or simple to save labor for effort and equipment therefor is saved. The microalga belongs to Chlamydomonas sp., and is a variant strain which has an ability to produce ethyl alcohol under dark and anaerobic conditions and has acquired an ability to proliferate while aggregating. The microalga is proliferated and maintained under dark and anaerobic conditions to generate ethyl alcohol in this method for producing ethyl alcohol.

A microbial oil includes a triglyceride. Sn-2 fatty acids of the triglyceride in the microbial oil contain 23% or more by weight of DHA. The microbial oil is prepared by fermentation using a Schizochytrium strain, where the Schizochytrium strain is Schizochytrium sp. with an accession number of GDMCC No. 60733. A food including the microbial oil is also provided.

Cement mixtures comprising a hydration retarder comprising algae, cement additives comprising algae and/or derivatives thereof, and methods of forming and using the mixtures and additives are disclosed. The algae can be treated to form desired functional groups to tune retardation properties of the hydration retarder.