The present disclosure provides a composition comprising: an extracellular polymeric substance produced by microalgae; plant growth promoting Gram-negative bacteria; and an agriculturally acceptable carrier. Also provided are an isolated biologically pure culture of Parachlorella kessleri Accession No. NCMA 202103001, a mutant thereof having all the identifying characteristics thereof, or a cell-free preparation or extracellular polymeric substance thereof, which may be used for plant enhancement and improving health of soil.

The invention is directed to Haematococcus sp. KAU-01 as well as to a culture medium for Haematococcus sp. KAU-01, and to methods for using this strain to process environmental pollutants such as gases generated by coal-fired plants.

The present invention is directed to isolated microorganisms as well as strains and mutants thereof, biomasses, microbial oils, compositions, and cultures; methods of producing the microbial oils, biomasses, and mutants; and methods of using the isolated microorganisms, biomasses, and microbial oils.

Provided is a component having plant defense activity. A PR1 gene expression activator containing a culture of a unicellular alga containing a green photosynthetic pigment as an active ingredient; A plant defense activator containing a culture of a unicellular alga containing a green photosynthetic pigment as an active ingredient; A plant disease preventive or ameliorating agent containing a culture of a unicellular alga containing a green photosynthetic pigment as an active ingredient; and a method of producing a plant defense activating component containing: cultivating a unicellular alga containing a green photosynthetic pigment under an aerobic condition to produce a culture containing an extracellular polysaccharide.

A system for growing algae includes a photobioreactor comprising a tubular structure having inner and outer surfaces, an annular space defined between the inner and outer surfaces, an inlet to allow an algae slurry to enter the annular space, and a mechanism configured to produce radially-directed contractions and expansions in the tubular structure. At least one of the inner and outer surfaces is transparent to at least some wavelengths of light useful for growing algae within the algae slurry.

The present invention can: efficiently and readily produce glucose from microalgae that accumulate starch in their cells; and obtain ethanol. During a preparation step of the glucose production method, microalgae are prepared on which a saccharifying enzyme acts on starch accumulated inside the microalgae cells, without disrupting the cell walls. In a saccharification step, starch inside the cells is saccharified and glucose is generated, by adding a saccharifying enzyme to the microalgae without a disruption treatment. The ethanol production method has a step in which, after the saccharification step, the glucose undergoes alcoholic fermentation and ethanol is generated.

A method for producing a liquid fertilizer based on liquid fertilizer quality certification (LFQC) of livestock manure, includes: subjecting a liquid released from livestock manure to high-temperature aerobic liquid-phase fermentation; subjecting the liquid resulting from the high-temperature aerobic liquid-phase fermentation to post-curing fermentation to produce a fermentation liquid fertilizer that complies with the standards of liquid fertilizer quality certification (LFQC) of livestock manure; and subjecting the produced fermentation liquid fertilizer to a separation membrane to produce a high-quality liquid fertilizer with reduced solid particles.

The present invention relates to a process for producing enriched algal biomass having high lipid productivity. More particularly, the present invention provides a process for obtaining an enriched biomass with omega-3 fatty acids by using a microalgal strain Schizochytrium MTCC 5980 in a unique media composition and substrate residual band in a steady state continuous fermentation. The process of the present invention results in high biomass and lipid productivity.

Use of aprotinin as a carrier to produce a recombinant protein, polypeptide or peptide in algae The present invention relates to the use of aprotinin as a carrier to produce a recombinant protein, polypeptide or peptide in algae, in particular microalgae, wherein aprotinin and said recombinant protein, polypeptide or peptide are fused together to form a fusion protein. It also relates to a method to produce a recombinant protein, polypeptide or peptide in algae, wherein said method comprises genetic transformation of algae, in particular microalgae, with a recombinant nucleic acid sequence encoding a fusion protein, wherein said fusion protein comprises aprotinin and said recombinant protein, polypeptide or peptide. It further relates to a recombinant algae comprising a recombinant nucleic acid sequence encoding a fusion protein, wherein said fusion protein comprises aprotinin and a recombinant protein, polypeptide or peptide. The use of said recombinant algae, for producing said fusion protein is also contemplated.

The present invention relates to a method for manufacturing microalgae micro powder containing astaxanthin and fatty acids with enhanced penetration performance and food availability, and more particularly, to a method for manufacturing microalgae micro powder containing astaxanthin and fatty acids with enhanced penetration performance and food availability, in which four kinds of functional microalgae are selected and mass-cultured so as to be processed into a dietary shape for easy penetration performance.