Provided herein are high oleic oil compositions. Further provided herein are methods of producing high oleic oil compositions from microorganisms and applications thereof in end products, including, for example, polyols, polyurethane products, personal care products, and food products.

A microorganism having at least one SPX gene encoding SPX protein responsive to phosphorus deficiency, and characterized in that a function of the SPX protein is decreased or lost by introducing gene mutation into the SPX gene.

Disclosed are techniques and systems for producing microbials having anaplerotic oils that are rich in odd-chain fatty acids, and other beneficial components, at higher concentrations than those present in other natural dietary sources of OCFA, at lower cost, and higher production yield. Further, disclosed are examples of incorporation of these higher concentration OCFA products into food for human and non-human animal consumption.

A microalgal strain of the species Nannochloropsis gaditana is characterized by a mutation of an enzyme involved in the biosynthesis of chlorophyll that changes the physiology thereof with respect to the wild-type strain of the same species. In particular, with the mutation, microalgae are formed which, with respect to the wild-type strain of the same species, have a lower chlorophyll content and a reduced capacity to absorb visible radiation (light).

Further, a process for the production of lipids through the cultivation of the mutated Nannochloropsis gaditana strain and the lipids obtained can be used as synthesis intermediates, particularly in the so-called “green-chemistry” sector or in the production of biofuels.

Provided is a component having plant defense activity. A PR1 gene expression activator containing a culture of a unicellular alga containing a green photosynthetic pigment as an active ingredient; A plant defense activator containing a culture of a unicellular alga containing a green photosynthetic pigment as an active ingredient; A plant disease preventive or ameliorating agent containing a culture of a unicellular alga containing a green photosynthetic pigment as an active ingredient; and a method of producing a plant defense activating component containing: cultivating a unicellular alga containing a green photosynthetic pigment under an aerobic condition to produce a culture containing an extracellular polysaccharide.

According to the invention, it has been found that when the amount of sulfate used in the culturing of PUFAs-producing cells is selected such that the sulfate concentration drops to zero in the last phase of the growth phase of the cells, an easily disruptable biomass is obtained which has an increased proportion by mass of polyunsaturated fatty acids (PUFAs) in the final product.

A novel alga mutant having improved pigment productivity is provided. The mutant can be used to produce carotenoid-based pigments, particularly, xanthophyll by using a small amount of energy, and thus can effectively produce pigment on an industrial level. In addition, the mutant can be used as a source for foods, health functional foods, and medications which contain a pigment. Further, when account is taken of the physiological characteristics of the euryhaline microalgae Dunaliella and the geological characteristics of Korean, the three sides of which are in contact with the sea, sea-water can be used as a culture medium, with the expectation of reducing the cost and leading to the development of related industries.

A bioreactor system for cultivating microalgae is described. The bioreactor system includes a bioreactor. The bioreactor includes one or more holes. One or more light sources are implanted into each of the one or more holes. A culture media comprising a carbon source is located inside of the bioreactor. A microalgae comprising a photoreceptor sensitive to a region of a visible spectrum is located in the culture media. Each of the one or more light sources produce an irradiance of light including the region of the visible spectrum in a sufficient intensity to transduce the photoreceptor of the microalgae.

Methods to extract chlorella protein from algae powder are described. A first extraction method is an alkaline solution extraction method. A second extraction method is an enzyme extraction method. A third method is a low-temperature deep eutectic solvents (DES) extraction method. According to each of these methods, a protein recovery rate is calculated from a protein extract solution of chlorella protein.

Disclosed is a process and system for generating hydrogen from carbon dioxide. The process and system for generating a hydrogen gas stream from a carbon dioxide gas stream comprises converting a first waste carbon dioxide gas stream to an organic feedstock using an algal source in a photosynthesis step. The organic feedstock is then converted using an organism to the hydrogen gas stream and gaseous by-products in a biodecomposition step. The generated hydrogen gas may then be collected.