The present application relates to a gRNA targeting CYP4V2 gene, and a donor nucleic acid molecule comprising a CYP4V2 gene fragment. The present application also relates to use of the gRNA and the donor nucleic acid molecule in the preparation of a medicament for treating Bietti crystalline dystrophy.

This invention relates generally to methods and compositions for producing a sesquiterpene alcohol comprising contacting a sesquiterpene with a P450 polypeptide with monooxygenase activity.

Metabolically-engineered yeast strains are provided. such as metabolically-engineered Saccharomyces cerevisiaestrains. producing high amounts of at least one. preferably all four natural cytokinins: trans-zeatin (tZ), trans-zeatin riboside (tZR). isopentenyladenine (iP) and isopenteny ladenine riboside (iPR).

Two novel cytochrome P450 genes are isolated from sorghum, each gene encoding a protein having pentadecatrienyl resorcinol hydroxylase activity. Expression vectors containing these sequences are made and used to elevate levels of pentadecatrienyl resorcinol hydroxylase in transgenic cells and organisms.

The present invention relates to a method for the production of a diterpene or a glycosylated diterpene, which method comprises: a. fermenting a recombinant microorganism in a suitable fermentation medium, wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol, whereby a diterpene or glycosylated diterpene is produced extracellularly in the fermentation medium; and b. recovering the diterpene or glycosylated diterpene from the fermentation medium.

This invention relates generally to methods and compositions for producing a sesquiterpene alcohol comprising contacting a sesquiterpene with a p450 polypeptide with monooxygenase activity.

The present disclosure relates to biosensing systems and biosensing elements having increased storage capability and increased functional lifetimes through using compositions and methods for recycling cofactors.

The present invention relates to a transgenic plant having an altered level of salicylic acid 3-hydroxylase (S3H) protein, compared to that of a non-transgenic plant, where the transgenic plant displays an altered leaf senescence phenotype, relative to a non-transgenic plant. The present invention relates to a mutant plant comprising an inactivated gene encoding S3H protein, where the mutant plant displays a premature or precocious leaf senescence phenotype, relative to a non-mutant plant. The present invention also relates to methods for promoting premature or precocious leaf senescence in a plant, delaying leaf senescence in a plant, and making a mutant plant having a decreased level of S3H protein compared to that of a non-mutant plant, where the mutant plant displays a premature or precocious leaf senescence phenotype relative to a non-mutant plant. The present invention also relates to inducing or promoting pathogen resistance in plants.

The present invention relates a process for preparing a cereal-based extract; said process comprises the steps of: (i) providing a whole grain cereal, (ii) subjecting the whole grain cereal to a single grinding and subsequently combining the whole grain cereal and water, (iii) subjecting the slurry of ground whole grain cereal and water to an enzymatic hydrolysis step, (iv) separating a soluble fraction of the whole grain cereal from an insoluble fraction through filtration, decantation or centrifugation and wherein the enzymatic hydrolysis step makes use of lytic polysaccharides monooxygeneases (LPMOs) in combination with carbohydrate hydrolysing enzymes and at least one protease. The invention also relates to a concentrated cereal-based extract ingredient comprising based on a dry matter basis, 40-90% (w/w) of carbohydrate, 5-20% (w/w) of protein, 0.5-20% (w/w) of total dietary fiber, optionally 1-4% (w/w) of ash and 0-10% (w/w) of fat, and such a extract obtained with the process.

The invention provides compounds, compositions, non-naturally occurring organisms, and methods useful for production of 5-hydroxytryptophan (5-HTP) in a microbial cell. A microbial system which includes at least one microbial cell, such as a bacterial cell or a yeast cell, is genetically engineered to express all or a portion of non-naturally occurring biosynthetic pathway that catalyzes the conversion of a simple carbon source, such as glucose, to 5-HTP. The invention can result in improved titers of 5-HTP and permits low-cost, large scale production. Methods of making and using the genetically engineered cells are also included in the invention.