The present invention relates to the technical field of biology, in particular to a phytase mutant, a preparation method therefor and an application thereof, a DNA molecule encoding the phytase mutant, a vector, and a host cell. The mutant provided by the present invention contains the substituent of an amino acid at at least one position selected from the following group: 36, 69, 89, 91, 111, 202, 213, 225, 238, 243, 253, 258, and 266. The heat resistance of the mutant is significantly improved, thereby facilitating the wide application of the phytase in feed.

Engineered robust high Tm-phytase clade polypeptides and fragments thereof are described herein. Also described are methods of making such engineered robust high Tm-phytase clade and fragments thereof and use thereof in enhancing animal performance.

Some embodiments relate to methods and kits for detecting and monitoring the potency of an enzyme encoded by an adeno-associated virus. Exemplary enzymes that may be used in conjunction with the compositions and methods of the disclosure are Myotubularin 1 (MTM1) and myotubularin-related proteins, such as myotubularin-related protein 1 (MTMR1), MTMR2, MTMR3, MTMR4, MTMR5, MTMR6, MTMR7, MTMR8, MTMR9, MTMR10, MTMR11, MTMR12, MTMR12, MTMR13, and MTMR14, among others.

The present disclosure concerns recombinant yeast host cells expressing cell-associated heterologous food and/or feed enzymes which are expressed during the propagation phase of the recombinant yeast hosts cells. The recombinant yeast host cells can be used in a subsequent production process to make food and/or feed products, for example, baked products.

The present invention relates to variant phytase enzymes and their use thereof.

Engineered robust high Tm-phytase clade polypeptides and fragments thereof are described herein. Also described are methods of making such engineered robust high Tm-phytase clade and fragments thereof and use thereof in enhancing animal performance.

Methods and materials related to producing glyceric acid and downstream products are disclosed. Specifically, isolated nucleic acids. polypeptides, host cells, methods and materials for producing glycolic acid by direct fermentation from sugars are disclosed.

A process for producing a protein isolate from an oilseed meal, and the isolate thus obtained, said isolate comprising proteins and an amount of 4 wt % or less of phytic acid, said amount of phytic acid being by weight of proteins in said isolate. The process may comprise the following steps: a) providing an oilseed meal; b) mixing the oilseed meal with a first aqueous solvent to form a slurry at a pH ranging from 6 to 7.8, said slurry having a solid phase; c) separating said solid phase from said slurry, d) mixing said separated solid phase with a second aqueous solvent at a pH ranging from 1 to 3.5, preferably from 2 to 3, to form a mixture said mixture having a liquid phase; e) separating said liquid phase from said mixture formed in step d); f) f1) mixing the separated liquid phase to a phytase at a temperature and a pH suitable for phytase activity to obtain a mixture having a liquid phase and a solid phase; and/or f2) mixing the separated liquid to a salt, to obtain a resulting liquid composition having a molar concentration of said salt ranging from 0.05 M to 0.5 M, at a temperature ranging from 40 C. to 70 C., to obtain a mixture having a liquid phase and a solid phase; g) precipitating a solid phase from the liquid of step f) for example by a cooling down step of the mixture to a temperature of 30 C. or less; h) separating said solid precipitate from the liquid of step g) said liquid comprising a water-rich liquid phase and an oil-rich liquid phase; i) separating said water-rich liquid phase from said oil-rich liquid phase, j) subjecting said water-rich liquid phase obtained in step i) to one or several membrane filtration(s) to obtain a protein isolate; and k) optionally, drying said protein isolate to obtain a dry protein isolate.

The present invention relates to the field of industrial fermentation and protein production. In particular, it relates to a method for producing a protein of interest in a fermentation medium comprising the following steps a) inoculating a fermentation medium with a Bacillus host cell comprising a gene encoding a protein of interest under the control of a promoter; b) cultivating the Bacillus host cell in the fermentation medium under conditions conducive for the growth of the Bacillus host cell and the expression of the protein of interest, c) adding sulfate to the fermentation medium to reach a concentration of at least 20 mM of sulfate in the fermentation medium; and d) allowing the protein of interest to precipitate and/or crystallize during cultivation; wherein the fermentation medium comprises an amino acid derivative in an amount of 0-30 g/l of fermentation medium. Further contemplated is the use of a combination of a sulfate and an amino acid derivative for producing a protein of interest in Bacillus host cell in a fermentation medium and a crystallized protein of interest obtained by or obtainable by the method of the invention.

The present disclosure relates to microorganisms useful in the biosynthesis of psicose. Also provided are methods of producing the disclosed microorganism and methods of producing psicose.