A method for conferring tolerance to a 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitor herbicide in a plant by expressing one or more polypeptide components of an exogenous plastoquinone-9 pathway in the plant. Nucleic acids, vectors, transgenic cells and transgenic plants useful in such a method are also disclosed.

The present invention provides for a genetically modified host cell comprising a first polypeptide comprising a sequence having at least 70% amino acid sequence identity with a phenylacetate decarboxylase, and having an enzymatic activity to decarboxylate a phenylacetic acid into a toluene and a carbon dioxide, and a second polypeptide comprising a sequence having at least 70% amino acid sequence identity with a phenylacetate decarboxylase activating enzyme, and having an enzymatic activity to cleave a S-adenosylmethionine (SAM) to form a methionine and a 5′-deoxyadenosyl radical.

Described are 3-methylcrotonic acid decarboxylase (MDC) variants showing an improved activity in converting 3-methylcrotonic acid into isobutene as well as methods for the production of isobutene using such enzyme variants.

Disclosed herein are prokaryotic and eukaryotic microbes, including E. coli and S. cerevisiae, genetically altered to biosynthesize tryptamine and tryptamine derivatives. The microbes of the disclosure may be engineered to contain plasmids and stable gene integrations containing sufficient genetic information for conversion of an anthranilate or an indole to a tryptamine. The fermentative production of substituted tryptamines in a whole-cell biocatalyst may be useful for cost effective production of these compounds for therapeutic use.

With the aim of providing a method allowing production of an unsaturated hydrocarbon compound such as butadiene with high productivity and an enzyme used in the method, the present inventors introduced mutations involving amino acid replacement into various positions of a ferulic acid decarboxylase, and prepared multiple modified forms of the enzyme. Then, the present inventors evaluated those modified forms in terms of the catalytic activity for the production of butadiene, and found as a result that the catalytic activity was improved in the case where, for example, the amino acid at position 395 was glutamine, histidine, asparagine, leucine, isoleucine, methionine, lysine, serine, arginine, tyrosine, or phenylalanine.

Consumer product compositions comprising fatty acid photodecarboxylases and methods of using consumer products to provide a benefit by converting long chain fatty acids present in soils into alkanes.

The need for a hand-dishwashing composition which provides good sudsing and a good suds profile even in the presence of greasy stains comprising higher chain-length saturated and/or unsaturated fatty acid chains, as well as improved removal of such stains, is met by formulating the composition with a P450 fatty acid decarboxylase and a surfactant system.

Consumer product compositions having P450 fatty acid decarboxylases and methods of using said consumer products to provide a benefit by converting long chain fatty acids present in soils into terminal olefins.

A DNA expression construct comprising a polynucleotide encoding an unnatural UstD enzyme, the unnatural enzyme itself, and a method of making gamma-hydroxy amino acids by contacting an aldehyde-containing substrate, an amino acid, and the unnatural, purified UstD enzyme under conditions and for a time sufficient to react at least a portion of the aldehyde-containing substrate with at least a portion of the amino acid, to yield a gamma-hydroxy amino acid product.

The present disclosure provides microbial cells and methods of producing FOPPA resulting from unique biosynthetic pathways, including biosynthetic pathways based on the phenylalanine/tyrosine biosynthetic branch and biosynthetic pathways based on bacteria metabolism. In particular, the present invention provides methods of producing FOPPA in microbial cells. These methods provide a low-cost, sustainable, and environmentally friendly source for FOPPA.