A method for forming an enzymatic biosensor includes preparing a first deposition solution comprising an enzyme, placing a substrate in the first deposition solution, applying an electrical potential to a working electrode of the substrate to deposit the enzyme on the working electrode, placing the substrate in a second deposition solution comprising electro-polymerizable monomers, and passing a current through the working electrode to polymerize the monomers to form an electropolymerized polymer layer over the enzyme deposited on the working electrode.

A method for forming an enzymatic biosensor includes preparing a first deposition solution comprising an enzyme, placing a substrate in the first deposition solution, applying an electrical potential to a working electrode of the substrate to deposit the enzyme on the working electrode, placing the substrate in a second deposition solution comprising electro-polymerizable monomers, and passing a current through the working electrode to polymerize the monomers to form an electropolymerized polymer layer over the enzyme deposited on the working electrode.

A method of preparing a modified-metal surface by attaching a phosphorous-based acid to a surface of a metal: preparing a solution of the phosphorous-based acid in a protic solvent; immersing a strip of a metal work piece into the solution of the phosphorous-based acid, immersing a strip of a reference metal into the solution of the phosphorous-based acid, supplying a voltage for a duration of time, removing the metal work piece, cleaning the metal work piece, and drying the cleaned metal work piece under an inert atmosphere to obtain a modified metal work piece.

A method of preparing a modified-metal surface by attaching a phosphorous-based acid to a surface of a metal: preparing a solution of the phosphorous-based acid in a protic solvent; immersing a strip of a metal work piece into the solution of the phosphorous-based acid, immersing a strip of a reference metal into the solution of the phosphorous-based acid, supplying a voltage for a duration of time, removing the metal work piece, cleaning the metal work piece, and drying the cleaned metal work piece under an inert atmosphere to obtain a modified metal work piece.

A hydrogel is formed by a reaction which is induced, in an electrolytic solution, by an electrode product electrochemically generated by electrodes installed in the electrolytic solution. An apparatus including an electrolytic tank with a bottom surface on which a two-dimensional array of working electrodes is provided and a counter electrode installed in the electrolytic tank is prepared. An electrolytic solution containing a dissolved substance that causes electrolytic deposition of a hydrogel is housed in the electrolytic tank. By applying a predetermined voltage to one or more selected working electrodes of the two-dimensional array, a hydrogel with a two-dimensional pattern corresponding to the arrangement of the selected working electrodes is formed.

A hydrogel is formed by a reaction which is induced, in an electrolytic solution, by an electrode product electrochemically generated by electrodes installed in the electrolytic solution. An apparatus including an electrolytic tank with a bottom surface on which a two-dimensional array of working electrodes is provided and a counter electrode installed in the electrolytic tank is prepared. An electrolytic solution containing a dissolved substance that causes electrolytic deposition of a hydrogel is housed in the electrolytic tank. By applying a predetermined voltage to one or more selected working electrodes of the two-dimensional array, a hydrogel with a two-dimensional pattern corresponding to the arrangement of the selected working electrodes is formed.

An apparatus and method for electrochemically depositing a unitary layer structure using a reactor configured to contain an electrolyte solution with an anode array containing a plurality of independently electrically controllable anodes arranged in a two-dimensional array, a cathode, an addressing circuit configured to receive a signal containing anode address data and configured to output a signal causing an anode array pattern; and a first controller being a current controller configured to control a flow of current to the anode array; a second controller in communication with the addressing circuit, the current controller and the anode array, the second controller operable to communicate with the current controller to command the flow of current to each anode in the anode array causing an electrochemical reaction at the cathode to deposit a layer corresponding to the anode array pattern signal received from the addressing circuit; and a third controller configured to clear bubbles which have formed on the anode after a length of time of steady state deposition by controlling the flow of the electrolyte solution across the anode array surface..

An apparatus and method for electrochemically depositing a unitary layer structure using a reactor configured to contain an electrolyte solution with an anode array containing a plurality of independently electrically controllable anodes arranged in a two-dimensional array, a cathode, an addressing circuit configured to receive a signal containing anode address data and configured to output a signal causing an anode array pattern; and a first controller being a current controller configured to control a flow of current to the anode array; a second controller in communication with the addressing circuit, the current controller and the anode array, the second controller operable to communicate with the current controller to command the flow of current to each anode in the anode array causing an electrochemical reaction at the cathode to deposit a layer corresponding to the anode array pattern signal received from the addressing circuit; and a third controller configured to clear bubbles which have formed on the anode after a length of time of steady state deposition by controlling the flow of the electrolyte solution across the anode array surface..

A coated titanium material includes a titanium material and a coating film formed on a surface of the titanium material. A Ti-based oxide is included in an interface between the titanium material and the coating film. The Ti-based oxide is one or both rutile type TiO.sub.2 and Ti.sub.2O.sub.3. In a case where a cut surface of the coating film is formed by using a SAICAS method under conditions that a horizontal speed is 2 μm/s and a vertical speed is 0.1 μm/s, on the cut surface, an area percentage of the Ti-based oxide is 30.0% or more in a region having a distance of 15 μm from a reference line specified on the basis of a boundary line, which is an intersection line between the cut surface and the interface, to a coating film side.

A coated titanium material includes a titanium material and a coating film formed on a surface of the titanium material. A Ti-based oxide is included in an interface between the titanium material and the coating film. The Ti-based oxide is one or both rutile type TiO.sub.2 and Ti.sub.2O.sub.3. In a case where a cut surface of the coating film is formed by using a SAICAS method under conditions that a horizontal speed is 2 μm/s and a vertical speed is 0.1 μm/s, on the cut surface, an area percentage of the Ti-based oxide is 30.0% or more in a region having a distance of 15 μm from a reference line specified on the basis of a boundary line, which is an intersection line between the cut surface and the interface, to a coating film side.