The invention provides a replicable genetic package displaying a cyclic peptide having at least one intramolecular bond between amino acid side chains. Also provided are a method of preparing such a genetic package displaying cyclic peptides having at least one intramolecular bond. Further provided is a library of replicable genetic packages displaying cyclic peptides each having at least one intramolecular cyclic bond between amino acid side chains; and a method of producing such a library.

The invention provides efficient methods for combining single-substitution libraries of nucleic acids that span and encode proteins of interest and for selecting resultant mutant proteins after expression which have improved properties or characteristics.

Compositions and methods for gene editing are provided. The methods employ an oligo-based annealing mechanism that is rooted in the process of DNA replication rather than homologous recombination (HR). Oligo incorporation efficiencies are comparable and often exceed those of CRISPR/cas9 editing without the need for double strand breaks (DSBs). By relying on the multiplex annealing of oligos rather than DSBs the process is highly scalable across a genomic region of interest and can generate many scarless modifications of a chromosome simultaneously. Combinatorial genomic diversity can be generated across a population of cells in a single transformation event; genomic landscapes can be traversed through successive iterations of the process, and genome-wide changes can be massively parallelized and amplified through systematic strain mating.

Compositions and methods for gene editing are provided. The methods employ an oligo-based annealing mechanism that is rooted in the process of DNA replication rather than homologous recombination (HR). Oligo incorporation efficiencies are comparable and often exceed those of CRISPR/cas9 editing without the need for double strand breaks (DSBs). By relying on the multiplex annealing of oligos rather than DSBs the process is highly scalable across a genomic region of interest and can generate many scarless modifications of a chromosome simultaneously. Combinatorial genomic diversity can be generated across a population of cells in a single transformation event; genomic landscapes can be traversed through successive iterations of the process, and genome-wide changes can be massively parallelized and amplified through systematic strain mating.

The present disclosure discloses an antibody fragment library, method for preparing the library and its applications. The essential steps in construction of the library is devoid of any restriction enzyme. Emulsion based PCR has been used as an important tool for the construction and validation of the library. The method as disclosed in the present disclosure leads to construction of a library comprising at least 8 billion clones.

The present disclosure application relates to a construction method of a yeast display library (YSD), specifically to a construct for a yeast display library, an expression vector, a host cell and a construction method and use thereof. The yeast display library provided by the present application has high transformation efficiency and rich diversity.

The presently disclosed subject matter relates to “Randomized Configuration Targeted Integration” (also referred to herein as “Randomized Chain Targeted Integration”) (RCTI) strategies for the generation and identification of host cells capable of expressing recombinant proteins, e.g., monoclonal antibodies, as well as compositions derived from the same, e.g., bispecific antibodies, and other complex format proteins, e.g., membrane protein complexes and other difficult to express molecules.

The presently disclosed subject matter relates to “Randomized Configuration Targeted Integration” (also referred to herein as “Randomized Chain Targeted Integration”) (RCTI) strategies for the generation and identification of host cells capable of expressing recombinant proteins, e.g., monoclonal antibodies, as well as compositions derived from the same, e.g., bispecific antibodies, and other complex format proteins, e.g., membrane protein complexes and other difficult to express molecules.

The present invention discloses means, use and non-GM method for modulating proteins in microorganisms. This method comprising steps of providing a system comprising a plasma discharge source, the plasma; the plasma discharge electric field is in the range of about 200 to about 500 v/m; and a plasma modifying mechanism comprising (1) at least one magnetic material, and at least one piezoelectric material, and (2) at least one optical crystal material, the plasma modifying mechanism providing modified plasma output having frequencies in the range of about 3 KHz to about 30 KHz; and discharging the plasma towards the microorganisms in a pulsed profile; thereby modulating proteins from the target microorganisms. The invention also discloses means, use and non-GM method for de novo generating of proteins in microorganisms from within the proteome of the microorganisms. The method comprises steps of providing a system as defined above, and discharging the plasma towards microorganisms in a pulsed profile, thereby activating proteins from the target microorganisms to de novo generating of proteins in microorganisms from within the proteome of the microorganisms.

The present invention discloses means, use and non-GM method for modulating proteins in microorganisms. This method comprising steps of providing a system comprising a plasma discharge source, the plasma; the plasma discharge electric field is in the range of about 200 to about 500 v/m; and a plasma modifying mechanism comprising (1) at least one magnetic material, and at least one piezoelectric material, and (2) at least one optical crystal material, the plasma modifying mechanism providing modified plasma output having frequencies in the range of about 3 KHz to about 30 KHz; and discharging the plasma towards the microorganisms in a pulsed profile; thereby modulating proteins from the target microorganisms. The invention also discloses means, use and non-GM method for de novo generating of proteins in microorganisms from within the proteome of the microorganisms. The method comprises steps of providing a system as defined above, and discharging the plasma towards microorganisms in a pulsed profile, thereby activating proteins from the target microorganisms to de novo generating of proteins in microorganisms from within the proteome of the microorganisms.