Provided is method for producing a phage library displaying antibodies or antibody fragments, comprising providing a first polynucleotide containing LC, a second polynucleotide containing connexon, and a third polynucleotide containing HC, respectively introducing the first, second and third polynucleotides to first, second and third bacteria to obtain a light chain component bacterial library, a connexon component bacterial library, and a heavy chain component bacterial library, obtaining a light chain component plasmid, a connexon component plasmid, and a heavy chain component plasmid from the libraries, obtaining the released LC, released connexon and released HC from the plasmids, connecting the released display vector segments to form a connection product for display, introducing a third bacterium to obtain a display bacterial library, and using the display bacterial library to prepare the phage library for displaying the antibodies or antibody fragments. Also provided is a phage library produced according to the method.

The purpose of the present invention is to provide a cyclic peptide having any unit structure selected from the structures represented by the following formula (1):

—X.sup.1—X.sup.2—X.sup.3—X.sup.4—X.sup.5—  (1) (in the formula (1), X.sup.1 is I, V or L, or an N-alkylamino acid thereof, X.sup.2 is S or T, or an N-alkylamino acid thereof, X.sup.3 is K or an N-alkylamino acid thereof, X.sup.4 is W or an N-alkylamino acid thereof, and X.sup.5 is W, Y, or H, or K or an N-alkylamino acid thereof), or a pharmaceutically acceptable salt of the cyclic peptide.

The present invention relates to methods of selecting, screening, engineering, making and modifying antibodies that have improved bioavailability upon subcutaneous administration to a human. Antibodies and variant antibodies with improved bioavailability upon subcutaneous administration to a human are also described.

The present disclosure provides compositions and methods for accurately detecting mutations by uniquely tagging double stranded nucleic acid molecules with dual cyphers such that sequence data obtained from a sense strand can be linked to sequence data obtained from an anti-sense strand when sequenced, for example, by massively parallel sequencing methods.

The present disclosure provides compositions and methods for accurately detecting mutations by uniquely tagging double stranded nucleic acid molecules with dual cyphers such that sequence data obtained from a sense strand can be linked to sequence data obtained from an anti-sense strand when sequenced, for example, by massively parallel sequencing methods.

Kits and methods for enriching target nucleic acid sequences, such as nucleic acid molecules including the target nucleic acid sequence, and kits and methods for depleting target nucleic acid sequences, such as nucleic acid molecules including the target nucleic acid sequences. In an embodiment, the methods for enriching target nucleic acid sequences include selectively degrading single-stranded sample nucleic acid molecules, such as those that do not include the target nucleic acid sequences. In an embodiment, the methods for depleting target nucleic acid sequences include selectively degrading double-stranded sample nucleic acid molecules, such as those including the target nucleic acid sequence.

Provided herein are methods that enable parallel evaluation of multiple functional nucleic acids in individual cells or subpopulations of cells, in the context of incubation with other types of single cells. The key insight is concurrent measurement of polynucleic acids derived from small populations of at least two different cell types, such that function in one cell type is linked to the clonal identity of another cell. These methods simultaneously process thousands, millions, or more single cells or small populations of cells. The method integrates molecular, algorithmic, and engineering approaches. This invention has broad and useful application in a number of biological and medical fields, including immunology and drug discovery.

Compositions, kits and methods are described that comprise one or more constructs, each construct comprising a ligand attached or conjugated to a polymer construct, e.g., an oligonucleotide sequence, by a linker, each ligand binding specifically to a single target located in or on the surface of a cell. The polymer construct comprises a) an Amplification Handle; b) a Barcode that specifically identifies a single ligand; c) an optional Unique Molecular Identifier that is positioned adjacent to the Barcode on its 5′ or 3′ end; and d) an Anchor for hybridizing to a complementary sequence, e.g., for generation of a double-stranded oligonucleotide. These compositions are used in methods, including high throughput methods, for detecting one or more targets or epitopes in a biological sample. These compositions are also used in a high throughput method for characterizing a cell by simultaneous detection of one or more epitopes located in or on the cell and its transcriptome.

Compositions, kits and methods are described that comprise one or more constructs, each construct comprising a ligand attached or conjugated to a polymer construct, e.g., an oligonucleotide sequence, by a linker, each ligand binding specifically to a single target located in or on the surface of a cell. The polymer construct comprises a) an Amplification Handle; b) a Barcode that specifically identifies a single ligand; c) an optional Unique Molecular Identifier that is positioned adjacent to the Barcode on its 5′ or 3′ end; and d) an Anchor for hybridizing to a complementary sequence, e.g., for generation of a double-stranded oligonucleotide. These compositions are used in methods, including high throughput methods, for detecting one or more targets or epitopes in a biological sample. These compositions are also used in a high throughput method for characterizing a cell by simultaneous detection of one or more epitopes located in or on the cell and its transcriptome.

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and antigen screening. Polynucleotide processing may be useful for a variety of applications. Antigen screening may comprise the use of one or more engineered cells. Engineered cells may be useful for characterizing one or more analytes including, for example, a polypeptide antigen.