A microdeposition pin having a contact surface with at least one concave edge for creating microarrays and the like. The microdeposition pin may be used either alone or with a plurality of microdeposition pins in conjunction with a holder. The concave edge of the pin is especially adapted for helping to control the spreading of a deposited material. By selectively controlling the spread of the reagent composition from the microdeposition pin, the flow of the reagent composition from the deposition target area may be reduced. Sensor strips having raised substrate features with limited or no spreading of the reagent composition beyond the target area are disclosed.

A microdeposition pin having a contact surface with at least one concave edge for creating microarrays and the like. The microdeposition pin may be used either alone or with a plurality of microdeposition pins in conjunction with a holder. The concave edge of the pin is especially adapted for helping to control the spreading of a deposited material. By selectively controlling the spread of the reagent composition from the microdeposition pin, the flow of the reagent composition from the deposition target area may be reduced. Sensor strips having raised substrate features with limited or no spreading of the reagent composition beyond the target area are disclosed.

A device for the microstructured grafting of proteins onto a substrate, comprising a substrate (7), a layer comprising a polyethylene glycol and being placed on the substrate, a matrix (10) of micromirrors for propagating the light in a first pattern and for replacing the first pattern with a second pattern. The microfluidic circuit is filled so as to bring a first aqueous solution containing a first protein into contact with the layer, a first microstructured image of the first pattern being formed on the layer to photoprint the first protein on the layer, and the microfluidic circuit is adapted to replace the first aqueous solution with a second aqueous solution containing a second protein so as to bring the second aqueous solution and the layer into contact, the first pattern being replaced with the second pattern in order to photoprint the second protein on the layer.

A device for the microstructured grafting of proteins onto a substrate, comprising a substrate (7), a layer comprising a polyethylene glycol and being placed on the substrate, a matrix (10) of micromirrors for propagating the light in a first pattern and for replacing the first pattern with a second pattern. The microfluidic circuit is filled so as to bring a first aqueous solution containing a first protein into contact with the layer, a first microstructured image of the first pattern being formed on the layer to photoprint the first protein on the layer, and the microfluidic circuit is adapted to replace the first aqueous solution with a second aqueous solution containing a second protein so as to bring the second aqueous solution and the layer into contact, the first pattern being replaced with the second pattern in order to photoprint the second protein on the layer.

Provided in the present invention are a method for constructing a nucleic acid single-stranded cyclic library and reagent kit thereof. The method comprises the steps of using a transposase embedding complex to randomly break nucleic acids and connect a first linker; connecting a second linker at a gap; performing a first PCR reaction, wherein the 5 end of one of the primers has a first affinity tag, resulting in a product with two ends connected to different linker sequences; binding the product to a solid vector having a second affinity tag; degenerating and separating single strands having no affinity tag; and cyclizing the single strands.

Compositions, methods and systems are provided for isolating DNA having a modified or unnatural base. Circular DNA fragments, each comprising a double stranded DNA central region and single stranded regions on the ends of the double stranded regions, are obtained. Some of the fragments have one or more modified or unnatural base. The DNA fragments are treated with a primer and a polymerase such that the polymerase extends the primer to copy at least one of the strand of the double stranded region. This results in rendering the other strand single stranded. A binding protein or antibody that is specific to the modified or unnatural base is then used to isolate strands containing the modified or unnatural bases. Methods for loading such complexes onto substrates and for single molecule sequencing of such complexes are also provided.

The invention features methods of making devices, or platens, having a high-density array of through-holes, as well as methods of cleaning and refurbishing the surfaces of the platens. The invention further features methods of making high-density arrays of chemical, biochemical, and biological compounds, having many advantages over conventional, lower-density arrays. The invention includes methods by which many physical, chemical or biological transformations can be implemented in serial or in parallel within each addressable through-hole of the devices. Additionally, the invention includes methods of analyzing the contents of the array, including assaying of physical properties of the samples.

The invention features methods of making devices, or platens, having a high-density array of through-holes, as well as methods of cleaning and refurbishing the surfaces of the platens. The invention further features methods of making high-density arrays of chemical, biochemical, and biological compounds, having many advantages over conventional, lower-density arrays. The invention includes methods by which many physical, chemical or biological transformations can be implemented in serial or in parallel within each addressable through-hole of the devices. Additionally, the invention includes methods of analyzing the contents of the array, including assaying of physical properties of the samples.

Method and system for monitoring for a change in the amount and/or concentration of a pathogen in a pathogenic fluid. The method includes providing a pathogen-impermeable enclosure enclosing the pathogenic fluid, wherein the pathogenic fluid includes a pathogen and a carrier fluid. Additionally, the method includes acoustically monitoring for a change in the amount and/or concentration of the pathogen enclosed in the pathogen-impermeable enclosure. The acoustically monitoring for a change in the amount and/or concentration of the pathogen enclosed in the pathogen-impermeable enclosure includes generating acoustic radiation directed towards the pathogen-impermeable enclosure, transmitting the acoustic radiation through the pathogen-impermeable enclosure or reflecting the acoustic radiation by the pathogenic fluid, receiving the acoustic radiation transmitted through the pathogen-impermeable enclosure or receiving the acoustic radiation reflected by the pathogenic fluid, and analyzing the received acoustic radiation.

Methods for sampling reactor contents in parallel reactor systems are disclosed. The methods may be used to sample reactor contents in non-atmospheric (e.g., pressurized) reaction vessels.