A method of remediation of soil and groundwater containing hydrocarbons and halogenated compounds. The method includes introducing a remediation composition into the soil that includes: (a) a first bioremediation material including a first blend of organisms capable of degrading the hydrocarbons; (b) a second bioremediation material including a second blend of organisms differing from the first blend of organisms that is chosen for degrading the halogenated compounds; (c) an organic compound such as a complex carbohydrate (e.g., food grade starch); and (d) a third blend of organisms degrading the organic compound. The degrading of the organic compound breaks the complex carbohydrate into smaller molecules that are utilized by the microorganisms of at least one of the first and second bioremediation materials during the degrading of the hydrocarbons and the halogenated compounds. The first bioremediation composition typically includes activated carbon capable of adsorbing the hydrocarbons and the halogenated compounds.

A system and method for generating an incubated bacteria solution by heating a nutrient germinant composition and bacteria, including at least one species in spore form, to a preferred temperature a range of 35-50° C. for 2-60 minutes using exothermic chemical reaction heat. An incubated bacteria solution is preferably generated at or near a point-of-use in an aquaculture, agriculture, wastewater, or environmental remediation application. The nutrient-germinant composition comprises L-amino acids, optionally D-glucose and/or D-fructose, a buffer, an industrial preservative, and may include bacteria spores (preferably of one or more Bacillus species) or they may be separately combined for incubation. A first chemical contained in a pouch is activated by contact with a second chemical, water, or air in a flameless heater to initiate exothermic reaction to provide incubation heat. A potable, single-use incubation bag is configured to hold the flameless heater and a container of nutrient germinant composition and spores.

An activated sludge process for the treatment of municipal wastewater, particularly applicable to oxidation ditch activated sludge treatment systems which utilize a conventional race track or continuous loop reactor basin configuration. The process removes phosphorus and nitrogen from an activated sludge wastewater treatment system, with an anoxic cycle followed by an aerobic cycle, and followed by a surface wasting cycle until a low flow diurnal period is reached in a diurnal or twenty-four hour period. Automated microprocessor control system using “oxidation-reduction potential” (ORP) and “dissolved oxygen” (DO) as process variable inputs automate aerated and anoxic cycles to optimize phosphorus and nitrogen removal using the available carbon in the influent wastewater resulting in an energy efficient dynamic dissolved oxygen control during the aerated periods.

A method of generating hydrogen gas includes providing a colony of sulfur-reducing bacteria and a colony of sulfur-oxidizing bacteria. The colonies can be submerged in a body of water. The colony of sulfur-reducing bacteria can be used to convert at least a portion of sulfates present in the body of water to hydrogen sulfide. The colony of sulfur-oxidizing bacteria can be used to convert the hydrogen sulfide to sulfuric acid. The sulfuric acid can react with manganese to produce hydrogen gas and manganese sulfate.

A method of separating oxygen from a body of water includes providing a colony of denitrifying bacteria submerged in the body of water. The colony of denitrifying bacteria can be used to convert at least a portion of nitrogen oxides present in the body of water to nitrogen gas. The method can also include collecting the nitrogen gas and bubbling the nitrogen gas through a portion of water from the body of water to remove dissolved oxygen from the portion of water. This can form a mixture of the nitrogen gas and oxygen gas.

A method for modifying a wastewater treatment device, the method including: 1) fixing a filling support in a wastewater pool, where the filling support includes a support frame; the support frame includes a hollow shaft, a fixed ring, and at least one vane; 2) adjusting the height of the filling support, until the fixed ring is submerged below the liquid level of the wastewater pool, where the water intake velocity of the wastewater pool is no less than 0.25 m/s, whereby a water current is produced and drives the support frame to rotate; 3) adding a functional liquid into the hollow shaft; and 4) adding an enzyme mixture to the hollow shaft when the rotation speed of the support frame is less than a first preset value, until the rotation speed of the support frame reaches a second preset value.

Compositions and methods for soil and groundwater remediation are contemplated as comprising a zero valent metal having a metal sulfide surface layer and an organic compound operative to degrade to produce molecular hydrogen. Such compositions may also include particulate activate carbon and anaerobic bacteria operative to perform reductive dechlorination. These compositions synergistically operate to remove chlorinated hydrocarbons from contaminated soil and groundwater.

The present disclosure relates to an integrated wastewater treatment apparatus and method, the apparatus comprises a first reactor module, a second reactor module, a sedimentation module and a gas-liquid separation module, the first reactor module comprises a first reactor and an anoxic reaction zone, an aerobic reaction zone, a first gas-gathering pressurized layer, a first water inlet pipe and an aeration device; the second reactor module comprises a second reactor, a second water inlet pipe, an anaerobic reaction zone and a second gas-gathering pressurized layer; the sedimentation module comprises a third reactor and a water outlet pipe; the gas-liquid separation module comprises a gas-liquid separator, an exhaust pipe, a first riser pipe, a second riser pipe and a return pipe. The apparatus can give full play to the advantages of the autotrophic biological denitrification process, meet the biochemical treatment requirements of wastewater with low C/N ratio.

A method is described for recovering resources from a microbe supporting environment such as a water treatment system, comprising the steps of using microbial DNA sequencing to analyze the microbiome of the microbe supporting environment and identifying adjustments to the microbial content of the microbiome that will be useful in extracting resources from the microbe supporting environment such as a water treatment system, wherein the resources extracted can include, for example, methane released by microbes, nitrogen, phosphorus, or other contaminants generated by microbes, and/or clean water obtained by removing contaminants in a water treatment system.

An ecological slope protection has an efficient water purification function, built in a land-lake ecozone along the bank of a basin, wherein the body of the ecological slope protection extends into the water of the basin, and has a flexible mattress laid on the surface thereof; an ecological filter pool is provided below the flexible mattress and is isolated from the water of the basin by means of the pool body; the ecological filter pool is contained with a filler layer and a support layer, and the filler layer is supported by the support layer; the sewage pretreated via a sedimentation pool is introduced into the ecological filter pool and treated by the filler layer to clean up pollutants therein and reduce its eutrophication level; and the treated sewage is discharged from the ecological slope protection via the support layer and enters the basin.