The present invention has an object of providing a modified lipase having excellent stability and reactivity at a high temperature. Provided is a modified lipase with improved reactivity and/or stability at a high temperature, the modified lipase having an amino acid sequence including one or more amino acid substitutions which are selected from the group consisting of T130C-S153C, A249P, F259Y, S282P, S283Y and S300P in the amino acid sequence represented by SEQ ID NO: 1, or an amino acid sequence having 90% or more sequence identity therewith.

A system and method are each directed to generating a biogas including methane by bacterial digestion of waste materials under anaerobic conditions. The waste digestion system includes three processing stages and a biogas production unit. The waste material is provided in the form of a water based slurry including solid particles having a distribution of particle sizes. The three processing stages are configured to remove and/or process the solid particles in the slurry, such that the biogas production unit receives a feedstock enriched in solid particles having a particle size suited for efficient digestion. The method generally includes processing the waste material in the three processing stages, digesting the waste material under anaerobic conditions, thereby generating the biogas.

The invention relates to an apparatus, methods and applications to grow microorganisms on-site to treat contaminated environments. Furthermore, the invention refers to an apparatus that is designed to function under a wide range of environmental conditions including extreme cold, extreme heat and direct exposure to sunlight. Such environments normally reduce the shelf-life of the organisms in the holding chamber that feeds the fermenter where they are being grown. These environments can also lower the growth rate of the organisms in the fermenter causing diminished cell output. Quite often the optimum point of application for the organisms is outdoor and too far from structures with appropriate protection from ultraviolet radiation from the sun or from excessive cold or hot weather. The invention also claims a dark coating that blocks ultraviolet radiation and a special coating that reflects heat away from the bioreactor. In addition, the invention claims a flexible micropore diffuser that allows aeration while gently mixing the fermentation broth. Finally, the invention claims a membrane bacterial filter for the air supply which prevents pathogens from entering the fermentation chamber. Such membrane bacterial filter is very economical and easy to replace.

The present disclosure relates to a harmless and recycling treatment method for kitchen waste. The method includes: step S1: sorting the kitchen waste, deodorizing the kitchen waste, and then implementing a solid-liquid separation to the kitchen waste to obtain solid and filtrate; step S2: adjusting pH of the filtrate to 12-13, adding a demulsifier to the filtrate, standing still, recovering an upper layer of oil and obtaining a lower layer of clear liquid; drying and burning the solid obtained in the step S1, to obtain biochar; and step S3: implementing an advanced oxidation treatment and a biochemical treatment to the lower layer of clear liquid obtained in the step S2, to obtain water. The method can effectively recycle the kitchen waste into resources, alleviate the social and environmental problems caused by the current kitchen waste, and the method is suitable for popularization and application.

The system comprises a pre-denitrification unit, an anaerobic ammonia oxidation unit, an advanced denitrification unit and a Fenton unit. The pre-denitrification unit is configured for hydrolyzing suspended pollutants and soluble organic matters in wastewater into organic acids, oxidizing ammonia nitrogen into nitrate, and finally converting the nitrate into nitrogen and absorbing phosphorus. The anaerobic ammonia oxidation unit is configured for converting a part of ammonia nitrogen in the wastewater into nitrite nitrogen through short-cut nitrifying bacteria and reacting the ammonia nitrogen with the nitrite nitrogen through anaerobic ammonia oxidation bacteria to generate nitrogen. The advanced denitrification unit is configured for reducing nitrate nitrogen into nitrogen through a carbon source and removing residual ammonia nitrogen, COD.sub.Cr and BOD.sub.5. The Fenton unit is configured for removing refractory organic matters and metal ions and adjusting the pH value of discharged water, so that the discharged water reaches the standard.

Described is a microorganism having an excellent effect of reducing oils and fats, and particularly, Asterotremella humicola strain 2-141-1 (Accession No. NITE BP-02641) or a microorganism having the same microbiological properties as those of the foregoing strain. Also described is an effluent treatment method including bringing the microorganism into contact with an effluent including oils and fats.

A method and a ventilation system includes ventilation ducts, a control system and at least one spray nozzle. The at least one spray nozzle is configured to spray a liquid mist onto at least one portion of an inner surface of the ventilation system. The liquid mist contains a culture of microorganisms adapted for biologically treating fat, oil and grease present on at least one portion of the inner surface of the ventilation system, thereby providing for partial biodegradation of the fat, oil and grease present on the at least one portion of the inner surfaces of the ventilation system.

The present invention relates to microbial compositions useful in treating swimming pool water to reduce water evaporation.

Processes and system are provided for digesting biosolids in a multistage digestion system. The processes and system are well suited for producing Class A biosolids and biogas containing a high content of methane. Methods and systems are also provided for recovery of phosphorus from wastewater and biosolids.

There is described herein a consortium of micro-organisms comprising, consisting or consisting essentially of Rhizobium spp., Bacillus spp., and Pseudomonas spp.