Wastesolution treatment systems and methods are provided which may remove particulates and hydrocarbons from wastesolution. Embodiments may be used to treat scrubber wastesolution from exhaust gas cleaning (EGC) systems. Some embodiments may be used with terrestrial EGC systems and others may be used for maritime ship EGC systems. Certain embodiments remove free phase oil and particulates from the wastesolution. Some embodiments may utilize a biogenerator cultured with hydrocarbon degrading microorganisms to reduce the concentration of oils in the water. Certain embodiments may utilize a clarifier and filtration unit to remove contaminants in the water. The wastesolution may be recirculated through the system until the measured contaminant concentration drops below a threshold value. The treated wastesolution may be stored, reused, or may be safely discharged from the system.

Methods are provided for using a combination of nanomaterials and oil-degrading bacteria to detoxify a multiphasic liquid (e.g., an oil-water mixture) and to ameliorate the toxicity of oil to local organisms, e.g., meiobenthos, in or near the area of the multiphasic liquid. The methods can be utilized for oil recovery and environmental clean-up and detoxification after spills and discharges. Through synergistic combination of the nanomaterials with oil degrading bacteria, methods can ameliorate environmental damage due to the presence of oil in an area through increased activation of the bacteria as well as through removal of the oil.

The present disclosure relates to the technical field of microbial electrochemical technology, in particular to a graphene-magnetite conductive skeleton electrode, a preparation method and application thereof, and a method for treating petrochemical wastewater. In the present disclosure, the surface roughness of the graphite rod electrode can be increased by the conductive skeleton modified on the surface of the graphite rod electrode, which is beneficial to the enrichment of microorganisms. The increase in the load of microorganisms will mean the amount of electroactive microorganisms will also increase, which will further improve the electron transfer ability, and because the material of the modified layer is a conductive material, it is also more conducive to the transfer of electrons; at the same time, the conductive skeleton modified on the surface of graphite rod electrode can also further enhance the transmission distance of electrons because of the skeleton constructed.

The present invention relates to compositions and methods for the remediation of contaminated solids and liquids. In particular, embodiments of the present invention relate to the bioremediation of solids and liquids by a composition comprising a biocatalyst or mixture of biocatalysts. The present invention also relates to methods for producing the bioremediation compositions and methods for applying the bioremediation compositions to contaminated sites, including treatment, storage, and disposal facilities, as well as various contaminated water sources, such as aquifers and reservoirs.

The present invention discloses a microcapsule material capable of reducing pollution containing polycyclic aromatic hydrocarbons, and a preparation method and application thereof. The preparation method comprises the following steps: (1) culturing mycobacterium gilvum CP 13 in a bacteria culture liquid to obtain a bacterial broth, wherein the mycobacterium gilvum CP 13 was deposited in China General Microbiological Culture Collection Center (CGMCC) on Jul. 22, 2013 with a CGMCC number of CGMCC No. 7963; and (2) applying a calcium alginate and chitosan to encapsulate the bacterial broth in a microcapsule through layer-by-layer self-assembly to produce a microcapsule material capable of reducing pollution containing polycyclic aromatic hydrocarbons. The present invention produces a microcapsule material with the microorganic activity through layer-by-layer self-assembly, which has superior adaptability to the environment and good ability to reduce pollution containing polycyclic aromatic hydrocarbons. The microcapsule material of the present invention can be used in bioremediation of industrial wastewater containing polycyclic aromatic hydrocarbons and contaminated soil containing polycyclic aromatic hydrocarbons.

The present disclosure provides variant cytochrome P450 monooxygenase for the remediation of crude oil, PFAs, PCBs, and other organo-halides, polynucleotides encoding the variant cytochrome P450 monooxygenase, host cells expressing the variant cytochrome P450 monooxygenase, and methods of using the variant cytochrome P450 monooxygenase. Host cells can comprise the variant cytochrome P450 monooxygenase, almA (flavin binding monooxygenase), and xylE (catechol dioxygenase) for remediation of crude oil, PFAs, PCBs, and other organo-halides.

The invention relates to compositions comprising microbial strains and methods for use of the compositions in removing hydrocarbons from water and/or soil. More particularly, the invention relates to compositions of isolated Bacillus strains selected from the group consisting of isolated Bacillus strains Bacillus licheniformis MDG-1000 (NRRL No. B-67888), Bacillus pumilus MDG-1047 (NRRL No. B-67891), and Bacillus amyloliquefaciens MDG1607 (NRRL No. B-67666), or strains having all of the identifying characteristics of these strains, and combinations thereof, and methods for use of these strains for removing hydrocarbons from water and/or soil. The invention also relates to a method of removing oil in a cleaning application as described herein.

Provided are methods of petroleum sequestration, which use electrospun microtubes capable of sequestering the petroleum from the water, and method of depleting and optionally degrading petroleum from water using floating devices which comprise the electrospun microtube attached to a floating carrier.

Provided herein are compositions and methods that can remove, metabolize, or degrade a hydrocarbon in an area that is contaminated by hydrocarbons. Methods for bioremediation of an area such as an area of land, a body of water, or a shoreline that are contaminated by a hydrocarbon, such as from a crude oil spill are also described. The compositions and methods described herein can be used on natural flora and fauna as well as manmade materials that are contaminated by a hydrocarbon.

A strain of marine oil degrading bacteria, compounds obtained by fermentation and their uses are published. The name is Alcanivorax dieselolei T6-6, with preservation Number: CGMCC NO: 9033. It belongs to -proteobacteria, oceanospirillales, alkane degrading bacteria branch, alcanivorax category and diesel alcanivorax species. The said bacteria can be used to degrade and remove petroleum hydrocarbon, control environmental pollution, produce surfactants, reduce surface tension of water, and for other purposes; a compound can be isolated from secondary metabolites after fermentation, one of which is dieselolei T6-6, 2-amino-6-(N-2-carbonyl-4-tridecyl-cyclobutane) hexanoic acid, and it is a kind of lysine ester. The compound has emulsifying activity on different organics, antibacterial effects against Gram-positive bacteria and fungus, and a better cytotoxic activity.