The present invention relates to microbial carriers for wastewater treatment, and in particular, relates to an organic composite powder carrier and its application for strengthening biological denitrification in municipal wastewater treatment. The organic composite powder carrier is compounded by a microbial carrier with a relatively large equivalent particle size and an organic alternative carbon source in the form of ultrafine powder. The composite powder carrier in the present invention includes a dedicated organic alternative carbon source, such as polyhydroxyalkanoates (PHA). The organic alternative carbon source can exclude the competitive relationship between microorganisms to ensure that denitrifying bacteria exclusively obtain electron donors required for denitrification. The composite powder carrier strengthens biological denitrification in municipal wastewater treatment, significantly improves denitrification efficiency, realizes deep biological purification of wastewater and ensures that the subject effluent complies with the strictest domestic discharge standards.

The present disclosure provides a solid waste treatment method, including following steps: screening off masses having a particle size greater than 0.5 cm from solid waste; adding extraction agents to the solid waste and then heating and stirring so that the solid waste is fully dispersed in the extraction agents; placing the mixed extraction agent in a centrifuge and centrifuging the mixed extraction feeding liquid phase separated by centrifuging into a rectification tower for rectification and cooling, recovering residual oil substances in a rectification kettle, separating mixture of cooled extraction agents and water, continuously adding the separated extraction agents into an extraction kettle for cyclic extraction, and conveying sewage to a sewage treatment plant to treat and discharging the sewage after the sewage reaches a treatment standard.

A composition for remediation of soil and groundwater containing halogenated compounds. The remediation composition includes an elemental iron-based composition, which may include activated carbon capable of absorbing the halogenated compounds with numerous pores impregnated with elemental iron. The remediation composition further includes a first bioremediation material including a blend of one-to-many organisms capable of degrading the halogenated compounds. The remediation composition includes an organic compound or polymeric substance and a second bioremediation material including a blend of one-to-many organisms capable of degrading the organic compound or polymeric substance over time (e.g., 20 to 365 or more days to provide a time release substrate-creating material or platform) into smaller molecules or compounds used by the organisms in the first bioremediation material while degrading the halogenated compounds. The organic compound may be a complex carbohydrate such as food grade starch, chitin, or other complex carbohydrate such as one with low water solubility.

A composition for stimulating the production and excretion of a lignolytic enzyme in a microorganism for degrading harmful substances and/or in the manufacturing of easily degradable ester containing plastics or articles made of ester containing plastic. The composition mainly includes tributyrin, triolein, fish oil, 16-hydroxyhexadecanoic acid, n-aliphatic primary fatty alcohols, polycaprolactone, aliphatic polyesters, linolenic acid, linoleic acid, alpha linolenic acid, plant polyesters, cutin, cutin derivatives, cutin monomers, omega hydroxy acids, 16-hydroxy palmitic acid, 9,16-dihydroxypalmitic acid, 10,16-dihydroxypalmitic acid, C18-hydroxy oleic acid, 9,10-epoxy-18-hydroxy stearic acid, 9,10,18-trihydroxystearate, suberin, cork, fruit skins, vegetable skins, and their constituents and derivatives, hydroxy fatty acids, 16-hydroxy palmitic acid, 18-hydroxy stearic acid, juniperic acid, hexadecanol, linseed oil, perilla oil, amides, acetamide and N-acetyl amide, zinc, zinc salts, butyrate, acetate, lactate, manganese peroxidase, and carbamide peroxide.

The highway sponge-type composite side ditch carbon neutralization system of the present invention includes three units, i.e., a silt pre-sedimentation channel, a filtering and oil-absorbing channel and an ecologic purification channel which are connected horizontally and successively. These three units work together to jointly complete the low-impact development functions of runoff collection, guide and drainage, purification and utilization, carbon emission is reduced by adopting various technical measures, the carbon sink effect is improved, and the full-life-cycle carbon neutralization effect from raw material production, construction to operation can be realized.

A method, a system, and a wastewater treatment system includes a biological treatment zone for biologically treating fat, oil and grease by adding a culture of microorganisms for partial biodegradation of the fat, oil and grease and/or breaking down the fat, oil and grease. The wastewater system includes a control system, an air injection and distribution system for improving and controlling the biological treatment process and at least one sensor. The at least one sensor may be configured to obtain sensor data, audio data and/or image data, and the control system may be configured to determine the thickness of a layer of fat, oil and grease on the surface of the biological treatment zone.

A method for the anaerobic cultivation of microorganisms includes providing an aqueous solution having a pH value of 4.5 to 7.5 in a container, adding a substrate in a first substrate dosage to the aqueous solution, adding further elements to the aqueous solution, adding an inoculant with microorganisms to the aqueous solution, hermetically sealing the container, varying a temperature in a range from 40 to 80 degrees Celsius, taking a reference liquid sample and determining a first concentration of organic substance in the reference liquid sample, taking another liquid sample and determining another concentration of organic substance in the further liquid sample after the expiration of the first waiting time, if the further concentration of organic substance is smaller than 10 percent of the first concentration of organic substance, adding substrate in another substrate dosage, repeating until a sufficient amount of biomass is present in the container.

Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifications of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

Disclosed is a method for preparing the same. The method for preparing a carrier including ammonium oxidizing bacteria immobilized therein includes: preparing a PVA-alginate mixed solution containing PVA mixed with alginate; adding sludge containing ammonium oxidizing bacteria and sodium bicarbonate (NaHCO.sub.3) to the PVA-alginate mixed solution to obtain a foaming-beading solution; and dropping the foaming-beading solution to a saturated boric acid solution to obtain beads including sludge immobilized therein, wherein sodium bicarbonate (NaHCO.sub.3) is decomposed to produce carbon dioxide (CO.sub.2) which is discharged to the exterior of the beads to form pores in the beads, when the foaming-beading solution is dropped to the saturated boric acid solution to obtain beads including sludge immobilized therein.

Animal and poultry solid waste material are treated with a preferred lactobacillus organism, preferably plant derived, in a liquid medium, preferably non-chlorinated water for time sufficient to reduce odor a significant amount.