A nutrient-germinant composition to aid in spore germination and a method for increased spore germination efficiency. The composition comprises L-amino acids, D-glucose and/or D-fructose, a phosphate buffer, an industrial preservative, and may include bacteria spores or they may be separately combined for germination. The method comprises providing a nutrient-germinant composition and bacteria spores, preferably of one or more Bacillus species, and heating to a preferred elevated temperature range of 41° C. to 44° C. for an incubation period of around 2 to 60 minutes. The nutrient-germinant composition is preferably in a concentrated liquid form that is diluted just prior to initiating the germination/incubation method at the point of use. The method may also include dispensing a germinated spore solution to a point-of-use/consumption, such as animal feed, water, or bedding, or a wastewater system or drain.

A biological filler includes an embedding agent and an embedded complex. The embedded complex includes a scallop shell powder, 1,5-dihydroxyanthraquinone, and Thiobacillus denitrificans. The embedding agent includes a poly(vinyl alcohol)-sodium alginate blend membrane and a crosslinked composite membrane. The embedded complex is embedded by the embedded agent.

There is described here a method of desiccation of aerobic granules.

The present invention is directed to an anode including bacteria, a polymer, and a conductive material, wherein the bacteria, the polymer and the conductive material are deposited on at least one surface of the anode. Further provided is a microbial electrochemical system comprising the herein disclosed anode, and methods of using the same, such as for treating wastewater, hydrogen production, or generating electricity.

Disclosed is a method for improving biological production of hydrogen from protein-containing wastewater comprising two stages: ultraviolet radiation pretreatment of protein-containing wastewater and biological production of hydrogen under a neutral pH condition and intermediate temperature condition.

According to present disclosure, there is disclosed an algae growth and cultivation system that provides a cost-efficient means of producing algae biomass as feedstock for algae-based products, such as, biofuel manufacture, and desirably impacts alternative/renewable energy production, nutrient recovery from waste streams, and valued byproducts production. The system as discussed herein is an integrated systems approach to wastewater treatment, algal strains selection for byproducts production, and recycle of algal-oil extraction waste or additional algae harvested as feedstock for fertilizer production. Embodiments of a system as discussed herein present an economically viable algae production system and process that allows algae-derived products such as biofuels, fertilizer, etc. to compete with petroleum products in the marketplace.

A composition remediation of soil and groundwater containing halogenated compounds. The remediation composition includes an elemental iron-based composition, which may include activated carbon capable of absorbing the halogenated compounds with numerous pores impregnated with elemental iron. The remediation composition further includes a first bioremediation material including a blend of one-to-many organisms capable of degrading the halogenated compounds. The remediation composition includes an organic compound or polymeric substance and a second bioremediation material including a blend of one-to-many organisms capable of degrading the organic compound or polymeric substance over time (e.g., 20 to 365 or more days to provide a time release substrate-creating material or platform) into smaller molecules or compounds used by the organisms in the first bioremediation material while degrading the halogenated compounds. The organic compound may be a complex carbohydrate such as food grade starch, chitin, or other complex carbohydrate such as one with low water solubility.

A method of preparing a microorganisms-immobilized felt-based resin includes the following steps: providing a mixture of an acrylate monomer, an initiator, a solvent, and water; adding a felt to the mixture; initiating a polymerization reaction of the mixture to form a felt-based resin; and immobilizing microorganisms on the felt-based resin to form the microorganisms-immobilized felt-based resin.

A method of providing, maintaining and using a youthful added microbe population for the treatment of wastewater. A method to obtain Class A sludge without the need for disinfecting procedures.

The present disclosure provides a solid waste treatment method, including following steps: screening off masses having a particle size greater than 0.5 cm from solid waste; adding extraction agents to the solid waste and then heating and stirring so that the solid waste is fully dispersed in the extraction agents; placing the mixed extraction agent in a centrifuge and centrifuging the mixed extraction; feeding liquid phase separated by centrifuging into a rectification tower for rectification and cooling, recovering residual oil substances in a rectification kettle, separating mixture of cooled extraction agents and water, continuously adding the separated extraction agents into an extraction kettle for cyclic extraction, and conveying sewage to a sewage treatment plant to treat and discharging the sewage after the sewage reaches a treatment standard.