Methods of conducting ureolysis-induced calcium carbonate precipitation with a heat-treated cell preparation, methods for preparing the heat-treated cell preparation, and related materials. The methods of conducting ureolysis-induced calcium carbonate precipitation include precipitating calcium carbonate at a location by introducing urea, calcium, and a heat-treated cell preparation comprising active urease enzyme to the location. The urease enzyme hydrolyzes the urea to ammonium carbonate, and the calcium reacts with the carbonate to form a calcium carbonate precipitate at the location. The methods of preparing the heat-treated cell preparation include heating a urease-producing cell preparation at a temperature and for a time sufficient to inactivate at least a portion of the cells in the urease-producing cell preparation while maintaining at least some urease activity of urease made by the cells in the urease-producing cell preparation

Methods of conducting ureolysis-induced calcium carbonate precipitation with a heat-treated cell preparation, methods for preparing the heat-treated cell preparation, and related materials. The methods of conducting ureolysis-induced calcium carbonate precipitation include precipitating calcium carbonate at a location by introducing urea, calcium, and a heat-treated cell preparation comprising active urease enzyme to the location. The urease enzyme hydrolyzes the urea to ammonium carbonate, and the calcium reacts with the carbonate to form a calcium carbonate precipitate at the location. The methods of preparing the heat-treated cell preparation include heating a urease-producing cell preparation at a temperature and for a time sufficient to inactivate at least a portion of the cells in the urease-producing cell preparation while maintaining at least some urease activity of urease made by the cells in the urease-producing cell preparation

Disclosed are biopolymeric and biologically active mortars suitable for use in providing building materials having enhanced physical properties. Further disclosed are methods for making and using the disclosed materials.

Disclosed are biopolymeric and biologically active mortars suitable for use in providing building materials having enhanced physical properties. Further disclosed are methods for making and using the disclosed materials.

The present invention provides substantially water free aluminate cement paste compositions that exhibit improved freeze thaw resistance and are storage stable upon being activated on admixture with water or an aqueous liquid. The compositions comprise a deep eutectic solvent mixture of a polar organic carrier component, preferably, glycerol or another hydrogen donor, and an anhydrous cation containing component, such as a metal salt or an onium compound in a paste with an aluminate cement. The compositions can be kept for as long as 30 days or more at room temperature without stabilization and then used to make cementitious coating layers or waterproofing membranes.

The present invention provides substantially water free aluminate cement paste compositions that exhibit improved freeze thaw resistance and are storage stable upon being activated on admixture with water or an aqueous liquid. The compositions comprise a deep eutectic solvent mixture of a polar organic carrier component, preferably, glycerol or another hydrogen donor, and an anhydrous cation containing component, such as a metal salt or an onium compound in a paste with an aluminate cement. The compositions can be kept for as long as 30 days or more at room temperature without stabilization and then used to make cementitious coating layers or waterproofing membranes.

Compositions, methods, and uses of calcium carbonate-based composition are presented. The calcium carbonate-based composition includes a plurality of restructured calcium carbonate particles that has an average size of equal or less than 10 microns in diameter. Preferably, the calcium carbonate-based composition is generated by unstructuring the aragonite using an acid and a chelator and recrystallizing the unstructured aragonite in a customized form. Exemplary aragonite-based compositions include pavement compositions.

Compositions, methods, and uses of calcium carbonate-based composition are presented. The calcium carbonate-based composition includes a plurality of restructured calcium carbonate particles that has an average size of equal or less than 10 microns in diameter. Preferably, the calcium carbonate-based composition is generated by unstructuring the aragonite using an acid and a chelator and recrystallizing the unstructured aragonite in a customized form. Exemplary aragonite-based compositions include pavement compositions.

What is disclosed is a cellulose-based product and a method for producing the aggregate admix product that includes the steps of thoroughly hydrating cellulose fibers, mixing clay and mineral particulates with a liquid to produce an emulsion, adding the emulsion to the hydrated cellulose fibers, and thoroughly impregnating the cellulose fibers with components from the emulsion and producing an aggregate admix product. The aggregate admix product is combined with cement and a liquid to create a cementitious product that can be directly sprayed onto building structures.

What is disclosed is a cellulose-based product and a method for producing the aggregate admix product that includes the steps of thoroughly hydrating cellulose fibers, mixing clay and mineral particulates with a liquid to produce an emulsion, adding the emulsion to the hydrated cellulose fibers, and thoroughly impregnating the cellulose fibers with components from the emulsion and producing an aggregate admix product. The aggregate admix product is combined with cement and a liquid to create a cementitious product that can be directly sprayed onto building structures.