This document describes biochemical pathways for producing a difunctional product having an odd number of carbon atoms in vitro or in a recombinant host, or salts or derivatives thereof, by forming two terminal functional groups selected from carboxyl, amine, formyl, and hydroxyl groups in an aliphatic carbon chain backbone having an odd number of carbon atoms synthesized from (i) acetyl-CoA and propanedioyl-CoA via one or more cycles of methyl ester shielded carbon chain elongation or (ii) propanedioyl-[acp] via one or more cycles of methyl ester shielded carbon chain elongation. The biochemical pathways and metabolic engineering and cultivation strategies described herein rely on enzymes or homologs accepting methyl ester shielded aliphatic carbon chain backbones and maintaining the methyl ester shield for at least one further enzymatic step following one or more cycles of methyl ester shielded carbon chain elongation.

This document describes biochemical pathways for producing a difunctional product having an odd number of carbon atoms in vitro or in a recombinant host, or salts or derivatives thereof, by forming two terminal functional groups selected from carboxyl, amine, formyl, and hydroxyl groups in an aliphatic carbon chain backbone having an odd number of carbon atoms synthesized from (i) acetyl-CoA and propanedioyl-CoA via one or more cycles of methyl ester shielded carbon chain elongation or (ii) propanedioyl-[acp] via one or more cycles of methyl ester shielded carbon chain elongation. The biochemical pathways and metabolic engineering and cultivation strategies described herein rely on enzymes or homologs accepting methyl ester shielded aliphatic carbon chain backbones and maintaining the methyl ester shield for at least one further enzymatic step following one or more cycles of methyl ester shielded carbon chain elongation.

A preparation method of carboxylic acids or ketones using ozone, singlet state oxygen atom O(.sup.1D) or hydroxyl free radical is provided. The method includes: filling ozone, singlet state oxygen atom O(.sup.1D) and/or hydroxyl free radical to cycloalkanes or benzenes at a pre-determined temperature and a pre-determined pressure in the presence or absence of light irradiation to produce carboxylic acids or ketones. The reaction occurs at room temperature and atmospheric pressure without producing harmful side products. The preparation method is a simple, low energy consuming, and eco-friendly method.

A preparation method of carboxylic acids or ketones using ozone, singlet state oxygen atom O(.sup.1D) or hydroxyl free radical is provided. The method includes: filling ozone, singlet state oxygen atom O(.sup.1D) and/or hydroxyl free radical to cycloalkanes or benzenes at a pre-determined temperature and a pre-determined pressure in the presence or absence of light irradiation to produce carboxylic acids or ketones. The reaction occurs at room temperature and atmospheric pressure without producing harmful side products. The preparation method is a simple, low energy consuming, and eco-friendly method.

The invention relates to a method for the continuous production of a solution of salts, in particular for the production of hexamethylenediamine adipate and a device for implementing such a method. According to the invention, it is proposed to convert, in a first step, a substoichiometric quantity of alkane diamine in a ratio to the alkane dicarboxylic acid in water and, in a subsequent second step, to implement making-up with alkane diamine, adjustment of the stoichiometric ratios being effected via a pH value measurement at a constant temperature.

The invention relates to a method for the continuous production of a solution of salts, in particular for the production of hexamethylenediamine adipate and a device for implementing such a method. According to the invention, it is proposed to convert, in a first step, a substoichiometric quantity of alkane diamine in a ratio to the alkane dicarboxylic acid in water and, in a subsequent second step, to implement making-up with alkane diamine, adjustment of the stoichiometric ratios being effected via a pH value measurement at a constant temperature.