Compounds, composition, and kits are provided for use in methods for the assisted folding of protein fragments of a of choice point larger protein by means of induced proximity, forced by specific nucleic acid hybridizations between a target nucleic acid molecule and complementary nucleic acid molecules appended to the protein fragments of interest.

The present disclosure provides enynes, end-functionalized polymers, conjugates, methods of preparation, compositions, kits, and methods of use. The conjugates comprise at least (1) a peptide (e.g., an antibody), protein, nucleoprotein, mucoprotein, lipoprotein, glycoprotein, or polynucleotide; and (2) a polymer comprising a pharmaceutical agent. The conjugates may be useful in delivering (e.g., targeted delivering) the pharmaceutical agent to a subject in need thereof or cell or treating, preventing, or diagnosing a disease.

The present disclosure provides enynes, end-functionalized polymers, conjugates, methods of preparation, compositions, kits, and methods of use. The conjugates comprise at least (1) a peptide (e.g., an antibody), protein, nucleoprotein, mucoprotein, lipoprotein, glycoprotein, or polynucleotide; and (2) a polymer comprising a pharmaceutical agent. The conjugates may be useful in delivering (e.g., targeted delivering) the pharmaceutical agent to a subject in need thereof or cell or treating, preventing, or diagnosing a disease.

Provided herein are modified amino acids comprising a tetrazine groups according to Formula I: polypeptides, antibodies, payloads and conjugates comprising these modified amino acid residues derived from the modified amino acids, and methods of producing the polypeptides, antibodies, payloads and conjugates comprising the modified amino acid residues. The polypeptides, antibodies, payloads and conjugates are useful in methods of treatment and prevention, methods of detection and methods of diagnosis. ##STR00001##

Provided herein are modified amino acids comprising a tetrazine groups according to Formula I: polypeptides, antibodies, payloads and conjugates comprising these modified amino acid residues derived from the modified amino acids, and methods of producing the polypeptides, antibodies, payloads and conjugates comprising the modified amino acid residues. The polypeptides, antibodies, payloads and conjugates are useful in methods of treatment and prevention, methods of detection and methods of diagnosis. ##STR00001##

A visualization kit includes (i) a cyanoacrylate and (ii) a fluorescent agent containing at least one tetrazine of formula 1: ##STR00001##
wherein X and Y represent independently a nonionic, electron-withdrawing group selected from halogens, azide, cyano and O-A-R, in which A represents a single bond or a C1-C4 alkyldiyl and R is alkyl, alkenyl, alkynyl, cycloalkyl or epoxyalkyl, or an aziridine group, which is optionally substituted by alkyl or aryl. A method of cofugation and a method of visualizing traces on a substrate utilizing these components are also presented.

A visualization kit includes (i) a cyanoacrylate and (ii) a fluorescent agent containing at least one tetrazine of formula 1: ##STR00001##
wherein X and Y represent independently a nonionic, electron-withdrawing group selected from halogens, azide, cyano and O-A-R, in which A represents a single bond or a C1-C4 alkyldiyl and R is alkyl, alkenyl, alkynyl, cycloalkyl or epoxyalkyl, or an aziridine group, which is optionally substituted by alkyl or aryl. A method of cofugation and a method of visualizing traces on a substrate utilizing these components are also presented.

The present invention relates to novel heterocyclic compounds, to processes for preparation thereof and to the use thereof for controlling animal pests, which include arthropods and especially insects and acarids.

The present invention relates to novel heterocyclic compounds, to processes for preparation thereof and to the use thereof for controlling animal pests, which include arthropods and especially insects and acarids.

The present application relates to functionalized 1,2,4,5-tetrazine compounds. The compounds are useful in compositions and methods using bioorthogonal inverse electron demand Diels-Alder cycloaddition reactions for the rapid and specific covalent delivery of a “payload” to a ligand bound to a biological target.