A method of harvesting plant product from a plant in a single pass using a combine harvester is disclosed. In the method, the plant has a protein content gradient that varies along a height of the plant. The method includes identifying, along a longitudinally-extending stalk of the plant, an upper protein gradient of the plant including high protein plant product and a lower protein gradient of the plant including lower protein plant product, wherein the high protein plant product from the upper protein gradient of the plant meets a threshold protein content that is higher than that of the lower protein plant product. The method also includes separately and substantially simultaneously harvesting the high protein plant product from the upper protein gradient and the lower protein plant product from the lower protein gradient in the single pass, and isolating the high protein plant product from the lower protein plant product.

A method of extracting a cytoplasmic or periplasmic protein. The method comprises provision of a first cell suspension comprising cells, the cells containing a cytoplasmic or periplasmic protein of interest to be extracted, the first cell suspension having a first temperature, and heating of the first cell suspension to an operating temperature, at which operating temperature at least a fraction of the cells is subject to heat-induced lysis and at least a fraction of the cytoplasmic or periplasmic protein of interest to be extracted is not subject to irreversible denaturation. The heating of the first cell suspension comprises provision of an aqueous solution, the aqueous solution having a second temperature that is higher than the first temperature, and mixing of the first cell suspension with the aqueous solution, thereby obtaining a second cell suspension, the second cell suspension having a third temperature that is higher than the first temperature. A system for extracting a cytoplasmic or periplasmic protein. The system comprises, i.a., a static mixer.

Biological small molecules, proteins or nucleic acids (target molecules, TM) are isolated in from biological media such as blood serum, cytoplasm, nucleoplasm etc. by a novel process (mate and separate) involving the use of PEGylated recognition molecule (PEG-RM) with high specificity and binding for TM, affording a macromolecular complex PEG-RM.TM, from which the target protein can be obtained in pure form.

Ag-Fe3O4 immunomagnetic microsphere contains poly-D-lysine modified on the surface and S100β and/or MBP antibody linked by an amide bond. The Ag-Fe3O4 immunomagnetic microsphere can specifically capturing peripheral nerve tissue-derived exosomes. When the microsphere is used to extract nerve tissue-derived exosomes, the extraction yield of exosomes per unit volume of nerve tissue is high, and the nerve specificity is strong.

There is provided a cell culture matrix comprising a fungal derived protein. Also provided is a composition comprising the cell culture matrix as described herein, a cell culture system comprising the cell culture matrix as described herein, and a method of forming a cell culture matrix thereof.

The present invention relates to an amphiphilic compound having a dendronic hydrophobic group, a method for preparing the same, and a method for extraction, solubilization, stabilization, or crystallization of a membrane protein by using the same. The use of the compound according to the present invention leads to an excellent membrane protein solubilization effect and a stable storage of a membrane protein in an aqueous solution for a long time, and thus can be utilized for functional analysis and structural analysis of the membrane protein. Especially, the amphiphilic compound having a dendronic hydrophobic group showed very remarkable characteristics in the visualization of protein composites through an electronic microscope. The membrane protein structural and functional analysis is one of the fields of greatest interest in current biology and chemistry, and more than half of the new drugs that are currently being developed are targeted at membrane proteins, and thus the amphiphilic compound of the present invention can be applied to membrane protein structure studies closely related to the development of new drugs.

The present disclosure relates to methods of isolating and purifying synthetic block copolymer proteins via the application of solvents and high shear microfluidization methods.

The disclosure relates to systems and methods for improving the manufacturing of silk solutions and powders containing silk fibroin obtained from silkworm cocoons. The solutions and powders can be used to improve the post-harvest preservation of perishables and to improve the performance of packaging, including biodegradable packaging.

A connecting polypeptide has SEQ ID NO:73. A proinsulin polypeptide includes a mature insulin A-chain, a mature insulin B-chain, and a connecting peptide comprising SEQ ID NO: 73 linking the mature A-chain and the mature B-chain, wherein the connecting peptide is not a native human proinsulin C-peptide. The proinsulin polypeptides according to the invention can be made in high titers and in high purity.

The disclosure relates to systems and methods for improving the manufacturing of silk solutions and powders containing silk fibroin obtained from silkworm cocoons. The solutions and powders can be used to improve the post-harvest preservation of perishables and to improve the performance of packaging, including biodegradable packaging.