A method for increasing peptide fragmentation by labelling the peptide at the C-terminal end with a guanidinium group or other basic functional group and distinguishing isobaric amino acids and amino acid combinations of asparagine and glycine-glycine; glutamine and glycine-alanine; and/or glutamine and alanine-glycine, during polypeptide sequencing. The method involves: obtaining a peptide of interest and/or digesting a polypeptide of interest with a protease, such as pepsin, chymotrypsin or trypsin, or by chemical cleavage to produce shorter peptides; reacting the obtained and/or generated peptides with a coupling reagent to derivatize the free C-terminal carboxylic acid function of the peptides, thus adding a basic functional group rendering C-terminal peptide fragment ions detectable by mass spectrometry; selecting a charge state of 2+ or more, and fragmenting the derivatized peptides in a mass spectrometer under conditions effective to generate at least w ions; and detecting the w ions by mass spectrometry, and identifying derivatized peptides which incorporate the additional mass of the basic functional group.

The present disclosure relates to methods of producing and purifying synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers comprising these proteins that recapitulate many properties of natural silk.

Disclosed is a process for the purification of lipopolypeptide antibiotics from culture broths which comprises: a) removal of the mycelium from the broth; b) anion-exchange chromatography of the solution resulting from stage a), eluting with di- or trivalent ions; c) optional concentration of the purified fraction resulting from stage b); d) hydrophobic interaction chromatography of the fraction resulting from stage b) or c), eluting with C1-C4 alcohols; e) cation-exchange chromatography of the desired lipopolypeptide-enriched fraction resulting from stage d), eluting at a pH equal to or greater than the isoelectric point of the lipopolypeptide; and f) dialysis, concentration and freeze-drying or spray-drying of the purified lipopolypeptide.

Methods to extract chlorella protein from algae powder are described. A first extraction method is an alkaline solution extraction method. A second extraction method is an enzyme extraction method. A third method is a low-temperature deep eutectic solvents (DES) extraction method. According to each of these methods, a protein recovery rate is calculated from a protein extract solution of chlorella protein.

Provided are food products that are derived from non-animal sources that have one or more of the following: color, taste, nutritional content, and other qualities similar to those of dairy products and/or other types of food products. Also provided are processes for production of such dairy-like food products and/or other types of food product analogs.

A method for extracting animal-derived pulmonary surfactants, including forming an extract of an animal lung, forming a precipitate by mixing the extract of the animal lung with a cationic flocculant solution containing poly(diallyldimethyl ammonium chloride) (pDADMAC), separating an organic phase containing pulmonary surfactants from the precipitate, recovering the pulmonary surfactants from the organic phase.

This invention relates to a process for the continuous production of vegetal protein concentrates by removing the soluble carbohydrates by an aqueous alcohol from a defatted vegetable material said process requiring no frequent production interruptions for cleaning operations. The innovative process includes the steps of removing at least a fraction of the soluble carbohydrates by solvent extraction, the solvent being aqueous alcohol, to obtain a solvent wet protein rich vegetable material and a miscella; removing the aqueous alcohol form the solvent wet protein rich vegetable material to obtain a vegetable protein concentrate; wherein the solvent extraction takes place in a moving screen solvent extractor equipped with a cleaning means of the moving screen on its return journey and for which no plugging takes place for extended period of time.

Provided are methods and compositions for isolating protein or other biomolecules from biological or environmental samples. The isolated biomolecules are substantially free of contaminants.

The present disclosure describes methods of obtaining and/or deriving proteins from plants of the Nicotiana species and methods for incorporation of such proteins into various products. For example, a method for obtaining a protein-enriched material from a plant of the Nicotiana species or portion thereof is provided, comprising: extracting one or more proteins from the plant material into a solvent to form a liquid protein-containing extract; separating a solid extracted plant material from the liquid protein-containing extract; clarifying the liquid protein-containing extract to form a clarified protein-containing extract and a solids fraction; and treating the clarified protein-containing extract so as to provide a protein-enriched material comprising at least about 60% protein by weight.

The present disclosure relates, according to some embodiments, to systems for purifying proteins and carbohydrate rich products from photosynthetic aquatic species and compositions thereof. In some embodiments, a system for recovering a highly soluble protein product from a biomass comprising a microcrop (e.g., Lemna) may comprise (a) a lysing unit to lyse a first portion of the biomass to form a first portion of lysed biomass, (b) a first separating unit to separate the first portion of lysed biomass to generate a first portion of a juice fraction and a first portion of a solid fraction, (c) a second separating unit to separate the first portion of the juice fraction to generate a first portion of a first juice and a first portion of a first cake, (d) a first filtration unit to filter the first portion of the first juice to generate a first portion of a soluble protein and a first reject stream, (e) a second filtration unit to filter the first portion of the soluble protein to generate a first portion of a second soluble protein and a second reject stream, (f) a dewatering unit to concentrate the first portion of the second soluble protein to generate a first portion of a concentrated soluble protein, and (g) a drying unit to dry the first portion of the concentrated soluble protein to generate a first portion of a dry protein concentrate.