Provided herein are methods for producing a mung bean protein isolate having high functionality for a broad range of food applications. In some embodiments, the methods for producing the isolate comprise one or more steps selected from: (a) extracting one or more mung bean proteins from a mung bean protein source in an aqueous solution, for example, at a pH between about 6.5-10.0; (b) purifying protein from the extract using at least one of two methods: (i) precipitating protein from the extract at a pH near the isoelectric point of a globulin-rich fraction, for example a pH between about 5.0-6.0; and/or (ii) fractionating and concentrating protein from the extract using filtration such as microfiltration, ultrafiltration or ion-exchange chromatography; and (c) recovering purified protein isolate.

Provided herein are methods for producing a mung bean protein isolate having high functionality for a broad range of food applications. In some embodiments, the methods for producing the isolate comprise one or more steps selected from: (a) extracting one or more mung bean proteins from a mung bean protein source in an aqueous solution, for example, at a pH between about 6.5-10.0; (b) purifying protein from the extract using at least one of two methods: (i) precipitating protein from the extract at a pH near the isoelectric point of a globulin-rich fraction, for example a pH between about 5.0-6.0; and/or (ii) fractionating and concentrating protein from the extract using filtration such as microfiltration, ultrafiltration or ion-exchange chromatography; and (c) recovering purified protein isolate.

Disclosed are two novel crystalline forms of S-acetyl glutathione (SAG) called Form A and Form B, obtained by crystallization of SAG from mixtures of water-acetone, water-ethanol or water acetone under controlled conditions. Forms A and B can be advantageously used as ingredients of pharmaceutical or nutraceutical formulations.

Tetrameric N-terminally acetylated α-synuclein is prepared by transforming an expression system with an expression vector encoding α-synuclein, wherein the expression system expresses a native NatB acetylase complex or ortholog thereof and/or wherein an exogenous NatB acetylase complex or ortholog thereof is co-expressed in the expression system, inducing protein expression in the transformed expression system, lysing cells in the transformed expression system to produce a cell lysate, performing salt precipitation of the cell lysate, recovering tetrameric N-terminally acetylated α-synuclein by centrifugation, and purifying the tetrameric N-terminally acetylated α-synuclein. Compositions comprising the same and methods for identifying compounds that stabilize natively folded tetrameric α-synuclein are also provided.

The present invention relates to a method for isolating caspofungin and to a novel crystalline form of caspofungin diacetate thus obtained.

Herein is reported a method for purifying cell cultivation supernatants either directly after fermentation or after one or more preliminary purification steps, such as protein A affinity chromatography. By adjusting the pH value in the acid range and subsequent incubation of the acidified solution host cell nucleic acid and host cell protein can be precipitated but the target polypeptide remains in solution. Thereafter the precipitate and therewith the contaminating host cell components can be removed by a simple physical separation step.

Herein is reported a method for purifying cell cultivation supernatants either directly after fermentation or after one or more preliminary purification steps, such as protein A affinity chromatography. By adjusting the pH value in the acid range and subsequent incubation of the acidified solution host cell nucleic acid and host cell protein can be precipitated but the target polypeptide remains in solution. Thereafter the precipitate and therewith the contaminating host cell components can be removed by a simple physical separation step.

Disclosed are a reagent kit for removing a bacterial endotoxin in a biological product, a method for using the reagent kit for removing the bacterial endotoxin in the biological product, a method for preparing an endotoxin-free biological product, and the endotoxin-free biological product thus produced. The reagent kit of the present invention comprises an anionic surfactant and a potassium salt. When in use, the anionic surfactant is fully bonded with the endotoxin in the biological product to form a conjugate, then the potassium salt is added to precipitate the conjugate, the precipitate is removed by filtration to produce a biological product solution with the endotoxin removed, and then the biological product is separated from the biological product solution to complete the process.

Disclosed are a reagent kit for removing a bacterial endotoxin in a biological product, a method for using the reagent kit for removing the bacterial endotoxin in the biological product, a method for preparing an endotoxin-free biological product, and the endotoxin-free biological product thus produced. The reagent kit of the present invention comprises an anionic surfactant and a potassium salt. When in use, the anionic surfactant is fully bonded with the endotoxin in the biological product to form a conjugate, then the potassium salt is added to precipitate the conjugate, the precipitate is removed by filtration to produce a biological product solution with the endotoxin removed, and then the biological product is separated from the biological product solution to complete the process.

A method for crystallizing insulin or insulin analogs under alkaline conditions and purifying the insulin or insulin analog crystals by filtering through a filter and drying the insulin or insulin analog crystals captured on the filter to produce crystalline insulin or insulin analog crystal compositions is described. In particular aspects, the method may be used to crystalize insulin glargine.