Processes for preparing and purifying protein from plant material, and compositions and uses comprising the same, are provided.

A device for obtaining protein-enriched fractions from human or animal milk comprises a delipidating unit for reducing a lipid content in the human or animal milk to obtain delipidated milk and a filtering unit for increasing a protein concentration of the delipidated milk to obtain the protein-enriched fraction, comprising a replaceable filter having a nominal molecular weight limit of 2 kDa or more, in particular of 5 kDa or more.

A device for obtaining protein-enriched fractions from human or animal milk comprises a delipidating unit for reducing a lipid content in the human or animal milk to obtain delipidated milk and a filtering unit for increasing a protein concentration of the delipidated milk to obtain the protein-enriched fraction, comprising a replaceable filter having a nominal molecular weight limit of 2 kDa or more, in particular of 5 kDa or more.

Described herein is a method for assessing disulfide bond reduction potential of a protein of interest comprising the following steps: Providing a cell culture fluid sample comprising mammalian cells expressing a protein of interest at a concentration within the range of between 0.2 g/l to 10 g/l Filtering said cell culture fluid sample over at least one filter Displacing O.sub.2 in the filtered cell culture fluid sample Collecting at least one sample of the O.sub.2-displaced filtered cell culture fluid sample Separating the proteins in said at least one O.sub.2-displaced, filtered cell culture fluid sample according to their size under non-denaturing conditions Determining the disulfide bond reduction potential of the protein of interest.

Described herein is a method for assessing disulfide bond reduction potential of a protein of interest comprising the following steps: Providing a cell culture fluid sample comprising mammalian cells expressing a protein of interest at a concentration within the range of between 0.2 g/l to 10 g/l Filtering said cell culture fluid sample over at least one filter Displacing O.sub.2 in the filtered cell culture fluid sample Collecting at least one sample of the O.sub.2-displaced filtered cell culture fluid sample Separating the proteins in said at least one O.sub.2-displaced, filtered cell culture fluid sample according to their size under non-denaturing conditions Determining the disulfide bond reduction potential of the protein of interest.

The present invention relates to a purification process of recombinant antibody fragments from inclusion bodies expressed in microbial cells. More particularly, the present invention relates to a process for purification of recombinant humanized (rHu) antibody fragment, Ranibizumab from inclusion bodies expressed in microbial cells.

Disclosed is a method for evaluating in vivo protein nutrition based on an LC-MS-MS technique, including the following steps: (1) collecting contents from different intestinal segments, and extracting and isolating protein ingredients; (2) determining the concentration of proteins; (3) treating before carrying out mass spectrometry: including digestion and desalting of a whole protein solution; (4) LC-MS-MS analysis; (5) database searching; and (6) data processing. Proteomic technology is used to identify proteins in the contents of different intestinal segments and digestive products thereof, and the source of the proteins in the contents of different intestinal segments and the contents thereof can be determined therefrom. Through bioinformatic analysis, the function of differential proteins in the body can be further understood, where the gene expression of enzymes related to protein digestion and metabolism may be different, thereby providing a scientific basis for further scientific evaluation of protein digestion and utilization.

The present disclosure provides improved systems and methods for purifying and/or concentrating lentiviral compositions.

The present disclosure provides improved systems and methods for purifying and/or concentrating lentiviral compositions.

This disclosure relates to a method which involves the steps of: (a) providing an aqueous solution comprising a protein and a polyalkoxy fatty acyl surfactant of general formula I

##STR00001##

wherein R.sup.1—C(═O) is a fatty acyl group, R.sup.2 is H or a substituted or unsubstituted hydrocarbyl group, X.sup.1 is S, O or NH, X.sup.2 is S, O or NH, n is 0 or an integer of 1-5, R.sup.3 is a polymeric group comprising polymerized units of general formula II and III

##STR00002##

(b) contacting the aqueous solution with a separation membrane, and (c) subjecting the aqueous solution to a diafiltration step and/or to an ultrafiltration step to produce a retentate product which is an aqueous solution comprising the protein, whereby the compound of formula I reduces aggregation of the protein in method steps (a)-(c) and whereby the compound of formula I passes through the separation membrane in step (c).