The present invention pertains to peptide-containing complexes/nanoparticles that are useful for delivering into a cell one or more mRNA (such as therapeutic mRNA, e.g., mRNA encoding a tumor suppressor protein).

Genetic recognition reagents comprising bivalent nucleobases are provided that bind two strands of nucleic acid, such as an expanded repeat sequence associated with an expanded repeat disease. In one example the expanded repeat disease is a polyQ disease, such as Huntington's disease. Methods of use of the reagents are provided, including a method of binding nucleic acid, such as an expanded repeat sequence associated with an expanded repeat disease, a method of identifying the presence of a nucleic acid comprising an expanded repeat, and a method of treating an expanded repeat disease are provided.

The present invention relates to -PNA monomers according to Formula I where substituent groups R.sub.1, R.sub.2, R.sub.3, R.sub.4, R.sub.5, R.sub.6, B and P are defined as set forth in the specification. The invention also provides methodology for synthesizing compounds according to Formula I and methodology for synthesizing PNA oligomers that incorporate one or more Formula I monomers.

The invention generally concerns a novel class of cyclopentane modified FIT-PNA (cpFIT-PNA) probes and uses thereof.

The invention relates to a microfabricated valve (10), comprising a first channel (11), a second channel (12) and a connection channel (13). The connection channel (13) connects the first channel (11) and the second channel (12). The microfabricated valve further comprises a valve portion (14) arranged within the connection channel (13), wherein the valve portion (14) is adapted to selectively open and close the connection channel (13). Moreover the invention relates to a method comprising the steps: inserting the first channel (11) into the first layer (21), inserting the second channel (12) into the third layer (23), inserting the connection channel (13) with the valve portion (14) into the second layer (22), and then arranging the second layer (22) between the first layer (21) and the third layer (23). The invention relates furthermore to a test device, in particular for a biological application and in particular a method for performing a biological test cycle.

According to one embodiment, a nucleic acid condensing peptide includes one terminal having a first sequence RRRRRR and another terminal having a second sequence RQRQR. Between the first sequence and the second sequence, none or one or more intermediate sequences each consisting of RRRRRR or RQRQR are included. Further, of the first sequence, the second sequence and the intermediate sequences, two or more neutral amino acids are included between any two sequences adjacent to each other.

The present disclosure relates to a fluorescent supramolecular biosensor, more particularly to a fluorescent supramolecular biosensor which shows change in fluorescence signal through binding to a specific bacterium and a method for preparing the same. The fluorescent supramolecular biosensor is advantageous in that it exhibits remarkably superior affinity and selectivity for a target bacterium. In addition, because a secondary coiled coil stereostructure is highly stabilized as the supramolecular building block is self-assembled to form the fluorescent supramolecular biosensor, the fluorescent supramolecular biosensor can be stored for a long period of time without structural change even at high temperatures.

Disclosed are compounds and compositions for the activation or induction of expression of a pattern recognition receptor (e.g., STING, RIG-I, MDA5), and methods of use thereof.

Selectively controllable cleavable linkers include electrochemically-cleavable linkers, photolabile linkers, thermolabile linkers, chemically-labile linkers, and enzymatically-cleavable linkers. Selective cleavage of individual linkers may be controlled by changing local conditions. Local conditions may be changed by activating electrodes in proximity to the linkers, exposing the linkers to light, heating the linkers, or applying chemicals. Selective cleaving of enzymatically-cleavable linkers may be controlled by designing the sequences of different sets of the individual linkers to respond to different enzymes. Cleavable linkers may be used to attach polymers to a solid substrate. Selective cleavage of the linkers enables release of specific polymers from the solid substrate. Cleavable linkers may also be used to attach protecting groups to the ends of growing polymers. The protecting groups may be selectively removed by cleavage of the linkers to enable growth of specific polymers.

The present invention relates in part to nucleic acids, including nucleic acids encoding proteins, therapeutics and cosmetics comprising nucleic acids, methods for delivering nucleic acids to cells, tissues, organs, and patients, methods for inducing cells to express proteins using nucleic acids, methods, kits and devices for transfecting, gene editing, and reprogramming cells, and cells, organisms, therapeutics, and cosmetics produced using these methods, kits, and devices.