Disclosed herein is a cosmetic composition including novel cell penetrating peptides, cell penetrating botulinum toxin recombinant proteins in which the cell-penetrating peptide and botulinum toxin are fused, polynucleotides encoding the cell penetrating botulinum toxin recombinant proteins, recombinant expression vector comprising the polynucleotides, and the cell penetrating botulinum toxin recombinant protein as active ingredients.

Disclosed herein are compositions and methods for treating Clostridium botulinum neurotoxin intoxication and in particular, vaccines against the neurotoxin that provide protection again lethal challenge with neurotoxin from one or more serotypes of Clostridium botulinum.

Disclosed herein are compositions and methods for treating Clostridium botulinum neurotoxin intoxication and in particular, vaccines against the neurotoxin that provide protection again lethal challenge with neurotoxin from one or more serotypes of Clostridium botulinum.

Chromatographic processes and systems for purifying a botulinum toxin from an APF fermentation medium.

Chromatographic processes and systems for purifying a botulinum toxin from an APF fermentation medium.

Method of producing an activated clostridial neurotoxin. Composition comprising an activated clostridial neurotoxin. Method of treatment using a composition comprising an activated clostridial neurotoxin.

Immunogenic compositions comprising isolated Clostridium difficile CDTb proteins, including modified proteins, and fusion proteins comprising a CDTa protein and a CDTb protein.

Immunogenic compositions comprising isolated Clostridium difficile CDTb proteins, including modified proteins, and fusion proteins comprising a CDTa protein and a CDTb protein.

The present invention relates to a botulinum toxin preparation method capable of obtaining botulinum toxin in a high yield through a simplified process that does not include animal-derived ingredients. The botulinum toxin preparation method according to the present invention does not use any animal ingredients in the overall process, including the culturing of a Clostridium botulinum strain, thereby providing excellent safety, omits a separate nucleic acid removal step using an additive treatment when compared with a conventional isolation process, and performs processing using only ion exchange chromatography, and it was confirmed that the botulinum toxin can be isolated at a remarkably improved yield through a simplified process by using the same buffer and only adjusting the concentration and pH of the buffer, and thus the present invention is a very economical and efficient isolation method so that the botulinum toxin isolated thereby is expected to be effectively used in beauty and medicine fields.

The present invention relates to a botulinum toxin preparation method capable of obtaining botulinum toxin in a high yield through a simplified process that does not include animal-derived ingredients. The botulinum toxin preparation method according to the present invention does not use any animal ingredients in the overall process, including the culturing of a Clostridium botulinum strain, thereby providing excellent safety, omits a separate nucleic acid removal step using an additive treatment when compared with a conventional isolation process, and performs processing using only ion exchange chromatography, and it was confirmed that the botulinum toxin can be isolated at a remarkably improved yield through a simplified process by using the same buffer and only adjusting the concentration and pH of the buffer, and thus the present invention is a very economical and efficient isolation method so that the botulinum toxin isolated thereby is expected to be effectively used in beauty and medicine fields.