The instant invention relates to filamentous fungal host cells producing and secreting a heterologous polypeptide of interest, said mutant host cell comprising and expressing a mutated ireA gene or a homologue thereof encoding a modified IreA polypeptide or a homologue thereof, said modified IreA polypeptide or homologue thereof comprising amino acid substitutions in 5 positions corresponding to positions 81 and 84 in the Aspergillus niger IreA amino acid sequence shown in SEQ ID NO:16, as well as methods of producing a polypeptide of interest in said cells and methods for constructing said cells.

The present disclosure provides a microbial genomic engineering method and system for transforming, screening, and selecting filamentous fungal cells that have altered morphology and/or growth under specific growth conditions. The method and system utilize high-throughput (HTP) methods to produce filamentous fungal production strains with a desired morphological phenotype.

The present disclosure provides a composition comprising (i) at least about 20% protein by dry weight, wherein said protein is not animal-derived protein, (ii) at least 20% by dry weight intact yeast cells, (iii) 20% to 70% plant-derived flour, (iv) at least 15% plant-derived starch selected from amylose, amylopectin and a combination thereof, (v) a vegetable oil, and (vi) a nutritional supplement comprising a plurality of vitamins and a plurality of minerals, wherein said composition has a water content that is less than or equal to about 30% water by weight, and wherein the product is produced by heat expanded extrusion.

The present disclosure provides a composition comprising (i) at least about 20% protein by dry weight, wherein said protein is not animal-derived protein, (ii) at least 20% by dry weight intact yeast cells, (iii) 20% to 70% plant-derived flour, (iv) at least 15% plant-derived starch selected from amylose, amylopectin and a combination thereof, (v) a vegetable oil, and (vi) a nutritional supplement comprising a plurality of vitamins and a plurality of minerals, wherein said composition has a water content that is less than or equal to about 30% water by weight, and wherein the product is produced by heat expanded extrusion.

Transgenic plant species engineered to inhibit aflatoxin production in Aspergillus species, methods of producing such transgenic plant species that can inhibit aflatoxin production, and compositions for such transgenic plant production. The transgenic plants harbor an RNAi cassette expressing dsRNA for an enzyme, such as aflC, that is required for aflatoxin biosynthesis. The dsRNA may include at least one, at least two, at least three, or more than three sections of the target enzyme gene.

A filamentous fungus mutant strain showing improved secretory protein production and a method of producing a protein using the filamentous fungus are provided. The method of producing a protein comprises a step of culturing a filamentous fungus mutant strain in which a function of tubulin is reduced or lost and collecting a protein from a culture product.

A filamentous fungus mutant strain showing improved secretory protein production and a method of producing a protein using the filamentous fungus are provided. The method of producing a protein comprises a step of culturing a filamentous fungus mutant strain in which a function of tubulin is reduced or lost and collecting a protein from a culture product.

The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions.

The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions.

Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganisms include at least one nucleic acid sequence encoding a xylulose kinase and/or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganisms are capable of growing on xylose as a carbon source.