The present disclosure is directed to methods for purifying prolamin proteins from a cereal flour containing such proteins. More particularly, the present disclosure provides a rapid and cost effective method for the purification of avenin proteins from oat flour, gluten proteins from wheat flour, secalin proteins from rye flour, hordein proteins from barley flour, zein proteins from maize flour or kafirin proteins from sorghum flour.

A method and system are disclosed for producing a zein protein product from a whole stillage byproduct produced in a corn (or similar carbohydrate-containing grain) dry-milling process for making alcohol, such as ethanol, and/or other biofuels/biochemicals. In one embodiment, the method includes separating the whole stillage byproduct into an insoluble solids portion and a centrate (solubles) portion, which includes protein, such as zein protein. Thereafter, the centrate (solubles) portion can be separated into a water soluble solids portion and a protein portion, which includes zein protein. Zein protein may be separated out from the protein portion. The remaining protein portion may be further processed to produce a high protein corn meal. The resulting zein protein portion may be further processed to be sold as a zein protein product and/or used as or in, for example, coatings, fibers, adhesives, ceramics, inks, cosmetics, textiles, food products, pharmaceutical, and biodegradable plastics.

A method and system are disclosed for producing a zein protein product from a whole stillage byproduct produced in a corn (or similar carbohydrate-containing grain) dry-milling process for making alcohol, such as ethanol, and/or other biofuels/biochemicals. In one embodiment, the method includes separating the whole stillage byproduct into an insoluble solids portion and a centrate (solubles) portion, which includes protein, such as zein protein. Thereafter, the centrate (solubles) portion can be separated into a water soluble solids portion and a protein portion, which includes zein protein. Zein protein may be separated out from the protein portion. The remaining protein portion may be further processed to produce a high protein corn meal. The resulting zein protein portion may be further processed to be sold as a zein protein product and/or used as or in, for example, coatings, fibers, adhesives, ceramics, inks, cosmetics, textiles, food products, pharmaceutical, and biodegradable plastics.

A method for separating one or more amino acids from a whole stillage byproduct produced in a corn dry milling process for making alcohol and system therefore is disclosed. In one embodiment, the method includes separating the whole stillage byproduct into an insoluble solids portion and a thin stillage portion, which include protein and amino acids. Thereafter, the thin stillage portion can be separated into a water soluble solids portion and a protein and amino acids portion. The resulting protein and amino acids portion can be separated into an amino acid(s) portion and a protein portion, both of which may be optionally dried, utilizing microfiltration, ultrafiltration, electrophoresis techniques/devices, or combinations thereof. The amino acid(s) in the resulting amino acid(s) portion may be further processed to be sold and/or used as or in, for example, nutritional supplements, flavor enhancers, animal feed additives, pharmaceuticals, industrial usage, fertilizers, herbicides, or as a feed source for other processing systems.

A method for separating one or more amino acids from a whole stillage byproduct produced in a corn dry milling process for making alcohol and system therefore is disclosed. In one embodiment, the method includes separating the whole stillage byproduct into an insoluble solids portion and a thin stillage portion, which include protein and amino acids. Thereafter, the thin stillage portion can be separated into a water soluble solids portion and a protein and amino acids portion. The resulting protein and amino acids portion can be separated into an amino acid(s) portion and a protein portion, both of which may be optionally dried, utilizing microfiltration, ultrafiltration, electrophoresis techniques/devices, or combinations thereof. The amino acid(s) in the resulting amino acid(s) portion may be further processed to be sold and/or used as or in, for example, nutritional supplements, flavor enhancers, animal feed additives, pharmaceuticals, industrial usage, fertilizers, herbicides, or as a feed source for other processing systems.

Compounds and compositions comprised of zein protein and a polypeptide of a protein extract, wherein the zein protein and the polypeptide of a protein extract are linked to each other are disclosed. Process of preparing the compounds compositions is also disclosed. Uses of the compositions as a nutritional supplement is also disclosed.

Compounds and compositions comprised of zein protein and a polypeptide of a protein extract, wherein the zein protein and the polypeptide of a protein extract are linked to each other are disclosed. Process of preparing the compounds compositions is also disclosed. Uses of the compositions as a nutritional supplement is also disclosed.

Described herein is a corn protein product comprising greater than about 20% corn protein on a dry weight basis and a free sulfite concentration of less than 150 ppm on an as-is basis. Also disclosed herein is a method to obtain this corn protein product including the steps of providing a protein-rich stream derived from a wet corn milling operation, wherein the protein-rich stream comprises greater than 20% corn protein on a dry weight basis, and treating the protein-rich stream with an oxidant, preferably hydrogen peroxide.

A protein product and an extraction method for making the protein product. Raw materials of the product optionally contains macromolecular carbohydrates and/or fat, and the method does not use organic solvents. The protein product contains prolamin and carbohydrates, wherein the prolamin accounts for 70 wt % or above of the protein (dry-basis), the -prolamin accounts for 75 wt % or above of the prolamin, the -prolamin accounts for 20 wt % or below of the prolamin, and the -prolamin accounts for 6 wt % or below of the prolamin.

A protein product and an extraction method for making the protein product. Raw materials of the product optionally contains macromolecular carbohydrates and/or fat, and the method does not use organic solvents. The protein product contains prolamin and carbohydrates, wherein the prolamin accounts for 70 wt % or above of the protein (dry-basis), the -prolamin accounts for 75 wt % or above of the prolamin, the -prolamin accounts for 20 wt % or below of the prolamin, and the -prolamin accounts for 6 wt % or below of the prolamin.