The present invention discloses a process for the secretion of brazzein in improved yield.

The present invention discloses a process for the secretion of brazzein in improved yield.

The present invention relates to a modified protein comprising an amino acid sequence that has one or more amino acids replacements from a reference protein, wherein the modified protein has at least one improved food-related property compared to the reference protein and to uses thereof in the food industry.

The present invention relates to a modified protein comprising an amino acid sequence that has one or more amino acids replacements from a reference protein, wherein the modified protein has at least one improved food-related property compared to the reference protein and to uses thereof in the food industry.

The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.

The present disclosure presents a solution to producing a sweet protein or a variant thereof having low or no calorie. By using recombinant gene and plant transformation techniques, non-native genes encoding a sweet protein are included in the propagatable genome of a plant, thereby forming a genetically modified plant, wherein the plant by the native genome thereof prior to modification may not produce the sweet protein naturally. Such genetically modified plant and a progeny thereof are enabled to produce non-native sweet protein and/or a variant thereof. Sweeteners, compositions, and consumables derived from the genetically modified plant are also provided.

The present disclosure presents a solution to producing a sweet protein or a variant thereof having low or no calorie. By using recombinant gene and plant transformation techniques, non-native genes encoding a sweet protein are included in the propagatable genome of a plant, thereby forming a genetically modified plant, wherein the plant by the native genome thereof prior to modification may not produce the sweet protein naturally. Such genetically modified plant and a progeny thereof are enabled to produce non-native sweet protein and/or a variant thereof. Sweeteners, compositions, and consumables derived from the genetically modified plant are also provided.

The present inventors confirmed that when a brazzein expression recombinant vector for high expression of brazzein in Saccharomyces cerevisiae was prepared and a S. cerevisiae strain Y2805 was transformed with the recombinant vector, the expression level of brazzein was particularly high, thereby completing an optimal expression system for mass-producing brazzein. Further, when the brazzein expression system is cultured under the optimal culture conditions according to the present invention, the amount of brazzein produced is further increased, the purification process is simple, and costs are reduced. Therefore, it is expected that the brazzein expression system according to the present invention can be widely used for mass-producing and commercializing brazzein, which is a sweet protein.

A secretion signal peptide sequence (SP) in combination with a cleavage inhibition sequence (CIS) fused to a structural gene sequence in a recombinant expression system can be used to express a full length protein with an SP in a cell. Such a fusion protein may be purified to homogeneity from a membrane fraction of the cell. The SP in combination with the CIS is a protein transduction domain that exhibits superior intracellular protein transduction efficiency when the SP precedes the CIS in a N to C-terminus direction.

The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.