The present invention provides a polypeptide capable of crossing the blood-brain barrier. In the present invention, C-terminal of the ziconotide is linked to N-terminal of a cell membrane penetrating peptide via one glycine to obtain a polypeptide capable of crossing the blood-brain barrier The polypeptide of the present invention is suitable for intravenous, intraperitoneal or nasal administration with convenient operation and low clinical risk. It has a long pharmacological effect in vivo, excellent analgesic effect, and slight peptide side effect after intravenous, intraperitoneal or nasal administration, and is suitable for large-scale clinical applications. The polypeptide of the invention has the advantages of simple preparation, controllable preparation process and quality during the preparation, and is suitable for large-scale industrial production.

Voltage reporter molecules and compositions, and methods for detecting voltage and voltage change in cells are provided. Also provided are methods for delivery, expression, and use of the voltage reporter molecules in cells, tissues, and subjects.

The present disclosure relates to compositions, methods, and kits for increasing the viability of bacteria that have been subjected to freeze-drying/lyophilization. In particular, the disclosure relates to compositions and methods for increasing the viability of living medicines (e.g., probiotics) during lyophilization, oral rehydration, and passage through the gastrointestinal tract

A preparation method and application of an anti-inflammatory and kidney-protecting clam peptide are disclosed. The method includes the steps of: cleaning and crushing the whole meat of a HonDau clam to obtain serous fluid, adding compound protease accounting for 0.1-0.3% of the weight of the serous fluid, carrying out enzymolysis, centrifugation, membrane separation and purification, and spray drying to obtain clam peptide powder. The clam peptide is prepared by adopting the HonDau clam, and is used for relieving the inflammatory reaction of an organism and the damage of renal function caused by hypertension, and is further applied to the inflammation and the damage of kidney caused by other diseases and health care foods with related functions.

A method of quantifying expression of a protein of interest with high temporal resolution; it includes providing a cell expressing a large fragment of a split fluorescent protein; transfecting the cell with a vector comprising a nucleic acid molecule comprising a first nucleic acid sequence encoding the protein of interest; a second nucleic acid sequence encoding the small fragment of the split fluorescent protein; and a third nucleic acid sequence encoding a linker protein that is cleaved during translation; quantifying expression of the protein of interest by detecting fluorescence resulting from a combining of the small fragment of the split fluorescent protein and the large fragment of the split fluorescent protein, wherein the linker protein is cleaved during the translation resulting in a stoichiometric ratio of the small fragment of the split fluorescent protein and the protein of interest.

The present invention relates to the field of biotechnology, molecular biology and medicine, in particular to nuclease enzyme and use thereof. More specifically, the present invention relates to PaCas9 nuclease enzyme. The invention also relates to a nucleic acid encoding said nuclease, a genetic construct, an expression vector, a delivery vector, which comprise said nucleic acid, a liposome comprising said nuclease or nucleic acid encoding said nuclease, a method for producing a nuclease, methods for delivery, and a host cell comprising a nucleic acid encoding said nuclease.

An antimicrobial adhesive protein, an antimicrobial nanoparticle, an antimicrobial composition comprising the same nanoparticle, and a preparation method for the same composition are described and, more particularly, an antimicrobial adhesive protein in which an antibiotic peptide is linked to a mussel adhesive protein, a mussel adhesive protein derivative of which a tyrosine residue within the antimicrobial adhesive protein is modified with a catechol derivative, an antimicrobial nanoparticle including a metal capable of forming a coordinate bond with a derivative of the mussel adhesive protein and having intrinsic antimicrobial activity, an antimicrobial composition comprising the same nanoparticle, and a preparation method for the same composition are described.

α-RgIA4 peptide analogs, compositions therewith, and methods for their treatment and use are disclosed and described. For example, an α-RgIA4 peptide analog can comprise a recognition finger region configured to bind to an α9α10 nicotinic acetylcholine receptor, and a side chain bonding configuration that protects an inter-cysteine sulfur linkage.

A diagnostically useful carrier includes a means for specifically capturing an antibody to a polypeptide from the group including squid MLC1 or squid MLC2 or a variant thereof in a sample from a subject. A method includes the step detecting in a sample from a subject the presence or absence of an antibody to squid MLC1 or squid MLC2. The polypeptide or the carrier or a polypeptide binding specifically to an IgE antibody from the sample of a patient to squid MLC1 or squid MLC2 are useful for the manufacture of a diagnostic kit, preferably for the diagnosis of allergy.

A protein memory cell and a protein memory system are provided. The protein memory cell includes: first and second electrodes disposed to be spaced apart from each other on a micro channel; a gap region defined between the first and second electrodes on the micro channel; an outer region defined as an opposite side to the gap region based on the first or second electrode on the micro channel; and a photosensitive protein changing conductivity between the first and second electrodes while moving between the gap region and the outer region depending on structural conversion of a chromophore.