The purpose of the present invention is to provide a method for producing a peptide that interacts with a lipid bilayer, and a lipid-bilayer-penetrating peptide obtained through the method. Provided is a lipid-bilayer-penetrating peptide constructed from 10 to 100 amino acids, the peptide having an amino acid sequence that penetrates the lipid bilayer at the C-terminal, and having an amino acid sequence with at least six contiguous arginine at the N-terminal. Also provided is a construct for producing a lipid-bilayer-penetrating peptide, the construct including, from the 5′ end toward the 3′ end, a tag region 1, a region 1 for incorporating a fluorescent protein gene sequence, a fluorescent protein gene region, a region 2 for incorporating a fluorescent protein gene sequence, a random region, and a stop codon region, and the construct being such that the random region has the aforementioned sequences.

The purpose of the present invention is to provide a method for producing a peptide that interacts with a lipid bilayer, and a lipid-bilayer-penetrating peptide obtained through the method. Provided is a lipid-bilayer-penetrating peptide constructed from 10 to 100 amino acids, the peptide having an amino acid sequence that penetrates the lipid bilayer at the C-terminal, and having an amino acid sequence with at least six contiguous arginine at the N-terminal. Also provided is a construct for producing a lipid-bilayer-penetrating peptide, the construct including, from the 5′ end toward the 3′ end, a tag region 1, a region 1 for incorporating a fluorescent protein gene sequence, a fluorescent protein gene region, a region 2 for incorporating a fluorescent protein gene sequence, a random region, and a stop codon region, and the construct being such that the random region has the aforementioned sequences.

The present invention provides a virus collection matrix, including: a porous gel or fibrous structure formed by a positively charged polymer material; and a plurality of ACE 2 receptors. The plurality of ACE 2 receptors are negatively charged, and distributed and covered on the surface of the porous gel or fibrous structure. The whole virus collection matrix is positively charged.

The present invention provides a virus collection matrix, including: a porous gel or fibrous structure formed by a positively charged polymer material; and a plurality of ACE 2 receptors. The plurality of ACE 2 receptors are negatively charged, and distributed and covered on the surface of the porous gel or fibrous structure. The whole virus collection matrix is positively charged.

Combination therapies for the treatment of cancer comprising an immunostimulatory fusion molecules that include an immune cell targeting moiety and a cytokine molecule; and an immune cell loaded with protein nanogels that include a reversibly crosslinked cytokine molecule and a polymer, pharmaceutical and formulations thereof, and methods of using and making the same, are disclosed.

Combination therapies for the treatment of cancer comprising an immunostimulatory fusion molecules that include an immune cell targeting moiety and a cytokine molecule; and an immune cell loaded with protein nanogels that include a reversibly crosslinked cytokine molecule and a polymer, pharmaceutical and formulations thereof, and methods of using and making the same, are disclosed.

Various embodiments are described herein for the fabrication enzyme degradable hydrogels useful as payload delivery systems. More particularly, embodiments disclosed herein relate to enzyme-degradable hydrogel systems comprising a crosslinkable polymer, such as a chemically-modified biopolymer, for example, chemically-modified gelatin, the hydrogel formed by a method comprising sequential physical and chemical crosslinking steps, for delivery of various payloads. Enzymes may be selected and administered to tune the release profile of the hydrogel. The payload can be, but not limited to, drugs, markers, cells, or these members encapsulated within another drug delivery such as a nanoparticle, or liposome. The hydrogel system can also be combined with another device such as a contact lens or bandage for wound healing.

Provided are a complex that comprises a nucleic acid-containing nanoparticle and a hollow particle of a non-enveloped virus, a method for producing the complex, and a pharmaceutical composition comprising the complex.

The present invention, as disclosed herein, provides an isolated peptide, and a composition or material comprising a hydrogel, for the delivery of an active agent. The hydrogel comprises one or more isolated peptides and an active agent encapsulated in the hydrogel. The hydrogel is at least partially in a β-sheet conformation. Further provided are a method for the encapsulation of an active agent in a hydrogel, a method for treating or diagnosing a condition or disease in a subject in need thereof.

The present invention relates to a method for producing Metal Organic Framework (MOF) having a framework that encapsulates a bio-molecule, the method comprising combining in a solution the bio-molecule and MOF precursors, wherein the bio-molecule promotes formation of the encapsulating framework.