The present invention relates to the field of proteases. More specifically, the present invention provides compositions and methods useful for profiling protease activity using phage display. In one embodiment, a display vector useful for profiling protease activity comprises a nucleic acid sequence encoding (a) a peptide to be displayed on the surface of the vector; (b) a first affinity tag C-terminal to the peptide; and (c) a second affinity tag N-terminal to the peptide. The display vector can comprise a virus, bacteriophage, yeast, bacteria, retrovirus, ribosome or mRNA. In particular embodiments, the peptide comprises a human peptidome library peptide.

The present invention relates to the field of proteases. More specifically, the present invention provides compositions and methods useful for profiling protease activity using phage display. In one embodiment, a display vector useful for profiling protease activity comprises a nucleic acid sequence encoding (a) a peptide to be displayed on the surface of the vector; (b) a first affinity tag C-terminal to the peptide; and (c) a second affinity tag N-terminal to the peptide. The display vector can comprise a virus, bacteriophage, yeast, bacteria, retrovirus, ribosome or mRNA. In particular embodiments, the peptide comprises a human peptidome library peptide.

Provided herein are peptides comprising an amino acid sequence having at least about 85% sequence identity to RYRPRAPIIAVT (SEQ ID NO: 1). These cationic peptides inhibit PKM2 methylation and may be used in the treatment of breast cancer and other diseases or conditions in which PKM2 is overexpressed. Such PKM2 peptides may be delivered to cancer cells using pH sensitive unimolecular nanoparticles comprising anionic polymers.

The invention discloses a coupled peptide chain for dissolving poorly soluble polypeptides and an application thereof for separation and purification in liquid chromatography, belonging to the field of biochemistry. A special linker arm is used to link a hydrophilic polypeptide chain with a poorly soluble polypeptide chain to solve the problem that the poorly soluble polypeptide chains cannot be operated in the liquid chromatography, and optimize the combination of hydrophilic amino acids, and then the poorly soluble polypeptide chain and hydrophilic polypeptide chain are broken by hydrolyzing an ester bond, so that the target peptide chain is directly precipitated, the method has the characteristics of simplicity and high efficiency, and the poorly soluble polypeptide product obtained by the method fully meets the standards required by customers.

The invention discloses a coupled peptide chain for dissolving poorly soluble polypeptides and an application thereof for separation and purification in liquid chromatography, belonging to the field of biochemistry. A special linker arm is used to link a hydrophilic polypeptide chain with a poorly soluble polypeptide chain to solve the problem that the poorly soluble polypeptide chains cannot be operated in the liquid chromatography, and optimize the combination of hydrophilic amino acids, and then the poorly soluble polypeptide chain and hydrophilic polypeptide chain are broken by hydrolyzing an ester bond, so that the target peptide chain is directly precipitated, the method has the characteristics of simplicity and high efficiency, and the poorly soluble polypeptide product obtained by the method fully meets the standards required by customers.

The present disclosure provides various molecular constructs having a targeting element and an effector element. Methods for treating various diseases using such molecular constructs are also disclosed.

The present disclosure provides various molecular constructs having a targeting element and an effector element. Methods for treating various diseases using such molecular constructs are also disclosed.

The present disclosure provides various molecular constructs having a targeting element and an effector element. Methods for treating various diseases using such molecular constructs are also disclosed.

The present disclosure provides various molecular constructs having a targeting element and an effector element. Methods for treating various diseases using such molecular constructs are also disclosed.

The invention discussed in this application relates to hydroxamic acid-based compounds that are useful as imaging agents when bound to an appropriate metal centre, particularly for the imaging of tumours.