Compositions and methods are provided for producing materials having increased immunoglobulin binding capacities, the materials including full-length or truncated forms of protein A, protein G, protein A/G, protein L and other immunoglobulin-binding proteins or peptides, which moieties contain polypeptide domains, or polypeptide-oligopeptide combinations. Also provided are separation matrices containing the moieties and methods of using the separation matrices for separation of immunoglobulins or immunoglobulin containing proteins.

Compositions and methods are provided for producing materials having increased immunoglobulin binding capacities, the materials including full-length or truncated forms of protein A, protein G, protein A/G, protein L and other immunoglobulin-binding proteins or peptides, which moieties contain polypeptide domains, or polypeptide-oligopeptide combinations. Also provided are separation matrices containing the moieties and methods of using the separation matrices for separation of immunoglobulins or immunoglobulin containing proteins.

A peptide-crosslinked protein-imprinted polymer, preparation method, and application thereof. One method comprises: 1) dissolving a main monomer, functional monomers, a peptide crosslinking agent, and a template protein in an aqueous solution to obtain a mixed solution; 2) adding an initiator or initiator system to the mixed solution to initiate the polymerization when the peptide crosslinking agent exists in a helix conformation to obtain a polymer; 3) eluting the template protein when the peptide chain exists in a coil conformation to obtain a peptide-crosslinked protein-imprinted polymer. The peptide crosslinking agent is a peptide with a polymerizable double bond at its both ends, and being capable of undergoing helix-coil transition. The polypeptide crosslinking agent is a polypeptide having an amino acid sequence which has a polymerizable double bond at its both ends, being capable of undergoing a helix-coil conformational transformation. The polypeptide cross-linked protein molecule-imprinted polymer disclosed in the invention not only can completely remove the template protein under mild conditions, but also can significantly improve the imprint effect of the protein molecule-imprinted polymer.

The present invention relates to heterotandem bicyclic peptide complexes which comprise a first peptide ligand, which binds to a component present on an immune cell, conjugated via a linker to a second peptide ligand, which binds to a component present on a cancer cell. The invention also relates to the use of said heterotandem bicyclic peptide complexes in preventing, suppressing or treating cancer.

A method for preparing a conjugate of a physiologically active polypeptide and a non-peptide polymer by linking physiologically active polypeptide with non-peptide polymer through a covalent bond using an organic solvent is provided. A method for preparing a physiologically active polypeptide complex by linking the conjugate with a carrier is provided. The complex shows improved in vivo duration and stability of the physiologically active polypeptide. The method can prepare the conjugate at a lower production cost, and the resulting conjugate shows an extension of in vivo activity at a relatively high level and significantly increase in the blood half-life.

A method for preparing a conjugate of a physiologically active polypeptide and a non-peptide polymer by linking physiologically active polypeptide with non-peptide polymer through a covalent bond using an organic solvent is provided. A method for preparing a physiologically active polypeptide complex by linking the conjugate with a carrier is provided. The complex shows improved in vivo duration and stability of the physiologically active polypeptide. The method can prepare the conjugate at a lower production cost, and the resulting conjugate shows an extension of in vivo activity at a relatively high level and significantly increase in the blood half-life.

The present invention relates to a supramolecular structure comprising a plurality of fused fibrils wherein each fibril comprises a plurality of cell adhesion motif lipopeptides. The invention also relates to an aqueous medium comprising said structure, a surface for cell maintenance, cell culture and/or cell bioprocessing wherein immobilised in or on the surface is said structure, as well as uses of said structure in cell maintenance, cell culture and/or bioprocessing.

The present invention relates to a supramolecular structure comprising a plurality of fused fibrils wherein each fibril comprises a plurality of cell adhesion motif lipopeptides. The invention also relates to an aqueous medium comprising said structure, a surface for cell maintenance, cell culture and/or cell bioprocessing wherein immobilised in or on the surface is said structure, as well as uses of said structure in cell maintenance, cell culture and/or bioprocessing.

Provided are β-eliminative linkers suitable for the conjugation of small molecule, peptide, and protein and compounds comprising the linkers.

The present disclosure provides antibodies specific for an epitope present on CD22. The antibodies are useful in various treatment, diagnostic, and monitoring applications, which are also provided.