The present invention provides a lectin-magnetic carrier coupling complex for separating glycosylated exosomes from a clinical sample. The lectin-magnetic carrier coupling complex comprises a magnetic carrier and lectins coupled to the outer side of the magnetic carrier. The lectin-magnetic carrier coupling complex provided by the present invention may rapidly, accurately, and automatically separate glycosylated exosomes from a clinical sample with a high separation efficiency; and the separated exosomes are intact in morphology without rupturing or cracking, may be directly used for liquid detection of glycosylated exosomes, or directly used for immunology-related detection, or directly used for nucleotide sequence detection and analysis after extracting nucleic acids from the exosomes.

The present invention provides a lectin-magnetic carrier coupling complex for separating glycosylated exosomes from a clinical sample. The lectin-magnetic carrier coupling complex comprises a magnetic carrier and lectins coupled to the outer side of the magnetic carrier. The lectin-magnetic carrier coupling complex provided by the present invention may rapidly, accurately, and automatically separate glycosylated exosomes from a clinical sample with a high separation efficiency; and the separated exosomes are intact in morphology without rupturing or cracking, may be directly used for liquid detection of glycosylated exosomes, or directly used for immunology-related detection, or directly used for nucleotide sequence detection and analysis after extracting nucleic acids from the exosomes.

In some examples, a CSS-MBs includes a solid magnetic core, a first shell material which surrounds the solid magnetic core and a second shell material which surrounds the first shell material. The first shell material may be a protective layer. The first shell material may include an inert carbon material. The second shell material may be have surface chemistry which allows for selective interaction of the CSS-MB with certain biomolecules under various buffer conditions.

In some examples, a CSS-MBs includes a solid magnetic core, a first shell material which surrounds the solid magnetic core and a second shell material which surrounds the first shell material. The first shell material may be a protective layer. The first shell material may include an inert carbon material. The second shell material may be have surface chemistry which allows for selective interaction of the CSS-MB with certain biomolecules under various buffer conditions.

The present invention provides a gentle and low cost means to isolate extracellular vesicles, including exosomes, from a surrounding sample. In particular the invention relates to the use of a fusion protein and biopolymer beads in such methods. Methods, compositions and medical uses of such compositions are also provided.

The present invention provides a gentle and low cost means to isolate extracellular vesicles, including exosomes, from a surrounding sample. In particular the invention relates to the use of a fusion protein and biopolymer beads in such methods. Methods, compositions and medical uses of such compositions are also provided.

The disclosure is directed to compounds and methods for preparing purified macrocyclic peptide using “catch-release” methods. These methods comprise reacting a free amino group of a resin-bound linear peptide with an azide- or alkyne-functionalized cap to form a resin-bound capped linear peptide having an azide- or alkyne-functionalized cap; cleaving the capped linear peptide from the resin to form a free capped linear peptide having an azide- or alkyne-functionalized cap; reacting the free capped linear peptide having an azide-functionalized cap with an alkyne-functionalized catch resin, or reacting the free capped linear peptide having an akynyl-functionalized cap with an azide functionalized catch resin, to form a catch-resin bound capped linear peptide; reacting the catch-resin bound capped linear peptide under conditions sufficient to effect macrocyclization of the linear peptide and release of the macrocyclic peptide from the catch resin.

The disclosure is directed to compounds and methods for preparing purified macrocyclic peptide using “catch-release” methods. These methods comprise reacting a free amino group of a resin-bound linear peptide with an azide- or alkyne-functionalized cap to form a resin-bound capped linear peptide having an azide- or alkyne-functionalized cap; cleaving the capped linear peptide from the resin to form a free capped linear peptide having an azide- or alkyne-functionalized cap; reacting the free capped linear peptide having an azide-functionalized cap with an alkyne-functionalized catch resin, or reacting the free capped linear peptide having an akynyl-functionalized cap with an azide functionalized catch resin, to form a catch-resin bound capped linear peptide; reacting the catch-resin bound capped linear peptide under conditions sufficient to effect macrocyclization of the linear peptide and release of the macrocyclic peptide from the catch resin.

A method for manufacturing a fiber assembly for providing a binding surface to a bio-substance is provided. A fiber assembly for providing a binding surface to a bio-substance according to an embodiment of the present invention is manufactured by a method comprising the steps of: (1) preparing a fiber assembly in which a plurality of fibers is accumulated; and (2) performing modification to provide the fiber surface with a carboxyl group reactive to an amine group present in a bio-substance. According to the method, a bio-substance can be easily introduced at a high content into the fiber assembly. In addition, bio-substances that are conjugated therebetween and adsorbed through physical adsorption, etc. are remarkably reduced in the introduction procedure of bio-substances, whereby bio-substances can be availed with high precision and reliability in applications employing bio-substances. Furthermore, applications employing bio-substances can undergo minimal property variations attributed to the bio-substances as detachment of the bio-substances is minimized or prevented. Accordingly, the bio-substances fixed on the surface of the fiber assembly according to the present invention can find a broad spectrum of applications in various fields including the material engineering, bio engineering, medical fields, and so on.

A method for manufacturing a fiber assembly for providing a binding surface to a bio-substance is provided. A fiber assembly for providing a binding surface to a bio-substance according to an embodiment of the present invention is manufactured by a method comprising the steps of: (1) preparing a fiber assembly in which a plurality of fibers is accumulated; and (2) performing modification to provide the fiber surface with a carboxyl group reactive to an amine group present in a bio-substance. According to the method, a bio-substance can be easily introduced at a high content into the fiber assembly. In addition, bio-substances that are conjugated therebetween and adsorbed through physical adsorption, etc. are remarkably reduced in the introduction procedure of bio-substances, whereby bio-substances can be availed with high precision and reliability in applications employing bio-substances. Furthermore, applications employing bio-substances can undergo minimal property variations attributed to the bio-substances as detachment of the bio-substances is minimized or prevented. Accordingly, the bio-substances fixed on the surface of the fiber assembly according to the present invention can find a broad spectrum of applications in various fields including the material engineering, bio engineering, medical fields, and so on.