There are provided efficient and cost-effective methods for purifying biomolecules in solution phase using stimuli-responsive protein-polymer conjugates. The protein-polymer conjugates comprise a target biomolecule-binding protein conjugated to a stimuli-responsive polymer and are reusable.

A thin film that has a flexible polymer layer and a peptide layer. The peptide layer is disposed on the flexible polymer layer and has a peptide selected from HYF, DYF and YFD. The thin film reversibly curves with changes in humidity.

The present invention relates to a multi-specific antibody conjugate (such as, a bispecific antibody conjugate), and a related composition, therapeutic method and use thereof. The antibody conjugate comprises a multispecific antibody, such as a bispecific antibody, conjugated to a nanomaterial. The antibody conjugate can be used to modulate an immune response, treat or prevent a disease or condition (e.g., a cancer, autoimmune disease, pathogen infection or inflammatory disease), etc.

The present invention relates to a multi-specific antibody conjugate (such as, a bispecific antibody conjugate), and a related composition, therapeutic method and use thereof. The antibody conjugate comprises a multispecific antibody, such as a bispecific antibody, conjugated to a nanomaterial. The antibody conjugate can be used to modulate an immune response, treat or prevent a disease or condition (e.g., a cancer, autoimmune disease, pathogen infection or inflammatory disease), etc.

A peptide-crosslinked protein-imprinted polymer, preparation method, and application thereof. One method comprises: 1) dissolving a main monomer, functional monomers, a peptide crosslinking agent, and a template protein in an aqueous solution to obtain a mixed solution; 2) adding an initiator or initiator system to the mixed solution to initiate the polymerization when the peptide crosslinking agent exists in a helix conformation to obtain a polymer; 3) eluting the template protein when the peptide chain exists in a coil conformation to obtain a peptide-crosslinked protein-imprinted polymer. The peptide crosslinking agent is a peptide with a polymerizable double bond at its both ends, and being capable of undergoing helix-coil transition. The polypeptide crosslinking agent is a polypeptide having an amino acid sequence which has a polymerizable double bond at its both ends, being capable of undergoing a helix-coil conformational transformation. The polypeptide cross-linked protein molecule-imprinted polymer disclosed in the invention not only can completely remove the template protein under mild conditions, but also can significantly improve the imprint effect of the protein molecule-imprinted polymer.

The present invention provides a polymer replica of a target molecule, the polymer replica being capable of accurately inheriting surface information possessed by the target molecule in a molecular imprinting technique. A polymer replica of a target substance, the target substance having multiple types of binding groups including at least a binding group BG1 and a binding group BG2 on the surface, wherein: the polymer replica is configured from a second molecularly imprinted polymer having, as a template, a first molecularly imprinted polymer having the target substance as a template; the polymer replica has on the surface thereof at least a binding group bg1 at a position corresponding to the position of binding group BG1 and a binding group bg2 at a position corresponding to the position of the binding group BG2 on the surface of the target substance; and the polymer replica of the target substance can be used as a template during synthesis of a molecularly imprinted polymer having a specific recognition site for the target substance because the polymer replica accurately inherits the surface information possessed by the target molecule.

The present invention provides a polymer replica of a target molecule, the polymer replica being capable of accurately inheriting surface information possessed by the target molecule in a molecular imprinting technique. A polymer replica of a target substance, the target substance having multiple types of binding groups including at least a binding group BG1 and a binding group BG2 on the surface, wherein: the polymer replica is configured from a second molecularly imprinted polymer having, as a template, a first molecularly imprinted polymer having the target substance as a template; the polymer replica has on the surface thereof at least a binding group bg1 at a position corresponding to the position of binding group BG1 and a binding group bg2 at a position corresponding to the position of the binding group BG2 on the surface of the target substance; and the polymer replica of the target substance can be used as a template during synthesis of a molecularly imprinted polymer having a specific recognition site for the target substance because the polymer replica accurately inherits the surface information possessed by the target molecule.

The present invention relates to reagents comprising a substituted acyl borate or a substituted hydroxylamine, to a method of synthesizing a carrier-linked prodrug using said reagents and to carrier-linked prodrugs obtainable by said method.

An object of the present invention is to provide a technique that allows other molecules/substances to be more selectively linked to the N-terminus in a simple and efficient manner, even in natural proteins etc. This object is achieved by reacting a compound represented by formula (1) or a salt thereof, or a hydrate or solvate of the compound or a salt thereof with a protein and/or peptide.

Described herein are nanoparticle-based compositions, kits and methods and platforms for delivering one or more nucleic acids to a cell.