The transgenic plants expressing one or more antagonist IL-10R peptides and anti-IL-10 single domain antibodies that stimulate or modulate the immune system and improve gastrointestinal physiology of an animal fed the transgenic plants or tissues thereof and the genes encoding the antagonist IL-10R peptides and anti-IL-10 single domain antibodies are described. The animal feed additives and animal feed incorporating the transgenic plants or tissues thereof are also described. Methods of stimulating or modulating an animal's immune system, improving an animal's gastrointestinal physiology, improving animal performance by using the transgenic plants or tissues thereof, and treating animals infected with a gastrointestinal pathogen are provided.

A method of making an antibody in plants that binds to ricin B chain is described. The method comprises (a) introducing a nucleic acid molecule encoding a heavy chain variable region of the antibody and a nucleic acid molecule encoding a light chain variable region of the antibody into a plant or plant cell; and (b) growing the plant or plant cell to obtain a plant that expresses the antibody or antibody fragment. The disclosure also relates to anti-ricin B chain antibodies and antibody fragments as well as methods of using same in therapy and prophylaxis.

Disclosed are monoclonal antibodies against Chikungunya virus having enhanced effector function and efficacy, methods of producing them, and methods of treatment of Chikungunya viral infection using the antibodies.

A method of recovering a product-of-interest from a plant or algal cell and a kit relating to the same are provided. The method of recovering a product-of-interest from a plant or algal cell includes (a) culturing a plant or algal cell that produces a product-of-interest in a culture medium; (b) adding functionalized nanoparticles to the culture medium, whereby the nanoparticles enter the plant or algal cell, bind the product-of-interest, and are extruded into the culture medium; (c) recovering the extruded nanoparticles from the culture medium; and (d) separating the product-of-interest from the extruded nanoparticles. The kit includes a plant or algal cell that produces a product-of-interest when in culture and a functionalized nanoparticle. Also provided is a method of separating plant or algal cells.

The transgenic plants expressing one or more antagonist IL-10R peptides and anti-IL-10 single domain antibodies that stimulate or modulate the immune system and improve gastrointestinal physiology of an animal fed the transgenic plants or tissues thereof and the genes encoding the antagonist IL-10R peptides and anti-IL-10 single domain antibodies are described. The animal feed additives and animal feed incorporating the transgenic plants or tissues thereof are also described. Methods of stimulating or modulating an animal's immune system, improving an animal's gastrointestinal physiology, improving animal performance by using the transgenic plants or tissues thereof, and treating animals infected with a gastrointestinal pathogen are provided.

The present invention relates to a method of producing a binder-toxin fusion protein comprising at least, one protein binder selected from the group consisting of an antibody, an antibody fragment or derivative retaining target binding capacity, or an antibody mimetic, optionally, a peptide linker, and at least one protein toxin or protein protoxin. The method comprises the steps of: contacting a plant cell or a whole plant with a nucleic acid construct comprising in operational linkage at least the following (A) at least one polynucleotide encoding for the protein binder, or a target binding chain or domain thereof, and either B1) a polynucleotide encoding for a cleavable peptide linker and a polynucleotide encoding for a protein toxin, or B2) a polynucleotide encoding for a protein protoxin, which protoxin comprises a cleavable domain for activation thereof, allowing the construct to integrate into the nucleus of the plant cell, or of one or more cells of the whole plant, and expressing the fusion protein encoded by the nucleic acid construct.

Expression vector for the stable production of a protein in plants, in particular a whole recombinant antibody in a cereal endosperm, comprising an expression cassette for the light polypeptide chain (L) of the antibody and an expression cassette for the heavy polypeptide chain (H) of the antibody, having the same orientation and the same control and regulatory elements of gene expression.

The present invention provides antibodies that neutralize MERS-CoV and methods of use thereof. The invented antibody is used to treat MERS-CoV infections and symptoms thereof.

The present invention describes the plant-based production of a therapeutic anti-virus antibody.

Provided is an application of a plant as a host in expressing a PD-1 antibody and/or a PD-L1 antibody, wherein the plant, such as lettuce, is used as an effective expression platform for preparing recombinant proteins, and a simple and effective agrobacterium-mediated vacuum infiltration method is used for expressing the PD-1 monoclonal antibody (Keytruda, pembrolizumab) and the PD-L1 monoclonal antibody (Atezolizumab).