The present application relates to glycosylated immunoglobulin domains. The invention provides nucleotide sequences encoding polypeptides comprising immunoglobulin variable domains with engineered glycosylation acceptor sites. Accordingly, the invention provides immunoglobulin variable domain proteins modified with selected glycans and specific glycan-conjugates thereof. Also provided herein are methods for the production of glycosylated immunoglobulin variable domains and glycan-conjugates thereof.

Provided herein are VHH-containing polypeptides that bind CD123. Uses of the VHH-containing polypeptides are also provided.

The invention relates to the field of biomedicine, and an anti-pertussis toxin (PT) single domain antibody and derivative protein thereof are disclosed. Specifically, a pertussis toxin-binding protein and use thereof are disclosed.

The present disclosure relates to extracellular vesicles, e.g., exosomes, comprising an AAV and a scaffold protein. In some aspects, the AAV is in the lumen of the extracellular vesicle. In some aspects, the AAV is associated with the luminal surface of the extracellular vesicle. In some aspects, the AAV is associated with the exterior surface of the extracellular vesicle. Also provided herein are methods for producing the exosomes and methods for using the exosomes to treat and/or prevent diseases or disorders.

The invention relates to the use of a single heavy chain variable domain antibody against human cytomegalovirus protein US28, which antibody binds to the extracellular region including, for example, the N-terminal extracellular region and/or the extracellular loops of US28, for isolation of cells that are infected with cytomegalovirus and/or for ex vivo reactivation of cytomegalovirus in latently infected cells. The invention further relates to the anti-US28 antibody for use in a method of reactivating cytomegalovirus in infected cells, or in a method of eliminating infected cells. The invention further relates to a tissue, organ, or cells such as bone marrow stem cells, from which cells that were infected with CMV have been removed with the use of the anti-US28 antibody.

The present disclosure provides improved CD33 targeting polypeptides and compositions for adoptive T cell therapies for treating, preventing, or ameliorating at least one symptom of a cancer, infectious disease, autoimmune disease, inflammatory disease, and immunodeficiency, or condition associated therewith.

The present disclosure provides improved CLL-1 targeting polypeptides and compositions for adoptive T cell therapies for treating, preventing, or ameliorating at least one symptom of a cancer, infectious disease, autoimmune disease, inflammatory disease, and immunodeficiency, or condition associated therewith.

A method is described for delivering a single domain antibody or a therapeutic molecule from an apical surface of a polymeric immunoglobulin receptor (pIgR)-expressing cell to a basolateral surface of the pIgR-expressing cell comprising contacting the pIgR-expressing cell with the single domain antibody or the therapeutic molecule, wherein the single domain antibody binds to pIgR, and the therapeutic molecule comprises an agent and the single domain antibody. A method is also described for transporting such a therapeutic molecule to systemic circulation or lamina propria or gastrointestinal tract of a subject comprising administering the therapeutic molecule to the subject via oral delivery, buccal delivery, nasal delivery or inhalation delivery.

An immunotherapy compound includes an NK cell engaging domain, an NK activating domain and a targeting domain. The targeting domain selectively binds to HER2, HERS, or the HER2/HER3 heterodimer complex and is operably linked to the NK activating domain and the NK cell engaging domain. The compound may be administered to a subject to induce NK-mediated killing of a cancer cell, to stimulate expansion of NK cells in the subject, and/or for treating cancer.

Disclosed are isolated single heavy chain variable (V.sub.HH) monoclonal antibodies, antigen binding fragments thereof, and bispecific antibodies include these V.sub.HH monoclonal antibodies, wherein the V.sub.HH monoclonal antibody or antigen binding fragment specifically binds a canine programmed death (PD)-1. Nucleic acid molecules encoding these V.sub.HH monoclonal antibodies and antigen binding fragments are also disclosed, as are expression vectors including these nucleic acid molecules and host cells including these expression vectors. Methods of detecting canine PD-1, and methods of increasing cytotoxic T cell activity and treating tumors are also disclosed.