The present invention is directed to a process for obtaining at least one of fucoidan and laminarin from live, harvested seaweed, wherein the process comprises the steps of: (i) harvesting seaweed comprising fucoidan and laminarin to obtain live, harvested seaweed, wherein the live, harvested seaweed exudes to form an exudate solution; (ii) collecting the exudate solution from the live, harvested seaweed, wherein the fucoidan and laminarin are dissolved in the exudate solution; and (iii) separating the at least one of fucoidan or laminarin from the exudate solution.

Therapeutic compositions and/or formulations are provided, comprising: at least one cross-linked protein matrix, wherein the at least one cross-linked protein matrix comprises at least one protein residue and at least one saccharide-containing residue, and methods of producing the same. The cross-linked protein matrix may be derived from cross-linking a full length or substantially full length protein, such as tropoelastin, elastin, albumin, collagen, collagen monomers, immunoglobulins, insulin, and/or derivatives or combinations thereof, with a saccharide containing cross-linking agent, such as a polysaccharide cross-linking agent derived from, for example, hyaluronic acid or a cellulose derivative. The therapeutic compositions may be administered topically or by injection. The present disclosure also provides methods, systems, and/or kits for the preparation and/or formulation of the compositions disclosed herein.

The present disclosure relates to method of obtaining high purity glucans, in particular yeast glucans with immunomodulatmy properties, including vaccine adjuvant properties for the use as adjuvant in vaccines.

Described herein is an isolated biological polysaccharide compound. The biological polysaccharide compound may be characterised by being isolated and having: glycosyl linkages comprising 1:3 linked glucopyranosyl residue of 65-95% wt and 1:6 linked glucopyranosyl residue of 5-25% wt; and a purity of 85-100% -glucan; and a molecular weight of 0.5 to 2.2 MDa; and a TNF-alpha cytokine response in a human bioassay that is at least 1.5 times greater than a negative control TNF-alpha cytokine response in a human bioassay; and being essentially insoluble in aqueous solutions. In at least one embodiment, methods are described of treating skin by topical application of a vehicle containing the isolated biological polysaccharide to a skin site such as a wound or burn. A method of manufacture is also described.

An adhesive composition contains a -1,3-glucan derivative having a degree of substitution of the acyl group of 2.6 or more and less than 3.0, and an isocyanate-based crosslinking agent. A pressure-sensitive adhesive tape includes a pressure-sensitive adhesive layer formed from the adhesive composition.

The present invention provides a process for the treatment of a composition comprising yeast cell walls comprising -1,3-glucans which are insoluble when extracted with water and partially soluble when extracted with DMSO, the process comprising contacting said composition with laminaripentaose-producing--1,3-glucanase and inactivating the laminaripentaose-producing--1,3-glucanase to result in a composition comprising yeast cell walls wherein the -1,3-glucans have an improved solubility in DMSO and the ratio of -glucans soluble in DMSO compared to water is greater than or equal to 2.

The invention provides a preparation method of high-content Ganoderma lucidum ?-glucan and an efficient and rapid detection method thereof. The preparation method comprises ethanol-trypsin pretreatment of Ganoderma lucidum fruiting body in combination with ultrasonic-assisted alkaline hydrothermal extraction of ?-glucan from Ganoderma lucidum, and purifying ?-glucan by DEAE Sephadex A-25 Sephadex column chromatography using NAOH solution as a mobile phase, to obtain high purity (?90%) of Ganoderma lucidum ?-glucan. The detection method comprises placing the extracted ?-glucan in an autoclave and performing hydrolysis with ?-(1,3)-exo-glucanase and ?-glucosidase, and detecting and analyzing the content of the prepared ?-glucan by high pressure-acid enzymatic hydrolysis-HPLC method, using glucose as a standard. The preparation method of ?-glucan has high extraction ratio. The efficient and rapid detection method has high sensitivity, high accuracy and good repeatability, and thus has a wide application prospect.

A method of extracting lentinan from lentinan containing mushrooms comprising the step of contacting the mushrooms with an ionic liquid so as to obtain a lentinan rich ionic liquid solution.

Reaction compositions are disclosed herein comprising at least water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and a beta-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with beta-1,3 glycosidic linkages. Further disclosed are methods of isolating beta-1,3-glucan.

The present disclosure relates to a biocompatible composition and a method for preparing the same, and a hybrid composition of -glucan and hyaluronic acid is prepared by hybrid cross-linkage. The biocompatible composition of the present disclosure high stability against heat and bio-enzymes, and thus is capable of being used as a medical material.