A method of preparing a Senna obtusifolia seed extract rich in anthraquinones and a galactomannan extract includes the following steps: (1) crushing Senna obtusifolia seeds into a Senna obtusifolia seed powder; (2) extracting the Senna obtusifolia seed powder with 40-85% ethanol, filtering to obtain an extract solution and a residue; (3) concentrating the extract solution under vacuum to obtain a concentrated extract solution, spray-drying the concentrated extract solution to obtain the Senna obtusifolia seed extract; (4) extracting the residue with membrane filtered water, conducting a centrifugation to obtain a supernatant; (5) adding ammonium sulfate and ethanol to the supernatant to form a two-phase aqueous system, collecting a bottom layer of the two-phase aqueous system; and (6) conducting an ultrafiltration of the bottom layer with a cut-off molecular weight of 50 k-200 k to obtain a galactomannan extract solution, drying the galactomannan extract solution under vacuum to obtain the galactomannan extract.

The present disclosure relates to an alkali metal salt of carboxyalkyl ether of tamarind gum with low fat content and high protein content and other elements like carbon, hydrogen and nitrogen at desired concentration levels. The modified alkali metal salt of carboxyalkyl ether of tamarind gum with improved composition of the present disclosure possesses high viscosity, cold water solubility, and desired particle size and purity levels and is biodegradable.

The present invention relates to a yeast-derived polysaccharide inducing Treg cells and a use thereof and, more particularly, to a polysaccharide comprising mannan and β-glucan, an composition for immunomodulation comprising the polysaccharide as an active ingredient, a pharmaceutical composition or food comprising the polysaccharide as an active ingredient for prevention or treatment of immune disease or inflammatory disease, a method for preparation of regulatory T cells by using the polysaccharide, a cell therapeutic agent comprising the regulatory T cells prepared by the preparation method as an active ingredient, and a treatment method using same. Even at a low dose, the novel polysaccharide according to the present invention allows the production of tolerogenic antigen presenting cells through the β-glucan and mannan structure retained therein, whereby the novel polysaccharide can induce the differentiation or production of antigen-specific regulatory T cells (Treg cells) to modulate the target immune system with low adverse effects. Therefore, MGCP and the Treg cells induced by the polysaccharide are effective for preventing or treating immune disease or inflammatory disease.

The present disclosure pertains to iodinated polysaccharide compounds that comprise a polysaccharide backbone that comprises a plurality of carboxyl groups and a plurality of iodinated side groups. The present disclosure also pertains iodinated polysaccharide compounds in which at least a portion of carboxyl groups that are present in a carboxyl-containing polysaccharide chain are functionalized with a plurality of iodinated side groups. Other aspects of the present disclosure pertain to methods of forming iodinated polysaccharide compounds, medical compositions comprising iodinated polysaccharide compounds, medical procedures comprising introducing such medical compositions into or between tissue of a patient, and medical kits that comprise such medical compositions.

Wine-making process comprising a first step of arranging “Acacia Verek” raw gum arabic and a second step of arranging a wine to be bottled, a step of dissolving the raw gum arabic in water in order to obtain raw gum arabic in solution with a concentration of gum arabic comprised between 5 and 50%, a step of filtering the raw gum arabic in solution by means of a tangential filter provided with synthetic membrane with nominal porosity comprised between 0.5 and 3 μm in order to obtain filtered gum arabic in solution with a concentration of gum arabic comprised between 5 and 40%, a step of admixing the filtered gum arabic in solution in the wine to be bottled in order to obtain a wine to be bottled admixed with gum arabic, and finally a step of microfiltrating the wine to be bottled admixed with gum arabic.

A method for producing a polysaccharide includes extracting from broccoli with hot water to obtain an extract, purifying the extract by a chromatography column, and hydrolyzing the purified extract with oxalic acid. The chromatography column can be a DEAE cellulose chromatography column. The polysaccharide produced can have innate immune stimulating activity.

A Dendrobium officinale oligosaccharide, a Dendrobium officinale oligosaccharide derivative and a preparation method and use thereof. The Dendrobium officinale oligosaccharide includes 3-9 glycoside residues and a glucose residue at a non-reducing end; the derivative thereof is obtained by substituting with a hydrophobic residue at the non-reducing end; the preparation method and use of both are also provided.

A substance having innate immune stimulating activity is a polysaccharide, which contains 25 to 50 parts by mole of galacturonic acid, 15 to 50 parts by mole of galactose, 0 to 7 parts by mole of glucose, 0 to 30 parts by mole of arabinose, 0 to 6 parts by mole of xylose, and 3 to 15 parts by mole of rhamnose as constituent sugars. The polysaccharide has a polygalacturonic acid chain having α-1,4-linked galacturonic acid as a main chain. An innate immune stimulant can include the polysaccharide as an active component, which can be included in a food or drink.

The present invention provides a new type of galacto-oligosaccharide, having a mannose residue instead of a glucose residue at the reducing end. The invention also relates to compositions comprising this galacto-oligosaccharide, its preparation and use in nutritional compositions.

A method of preparing a Senna obtusifolia seed extract rich in anthraquinones and a galactomannan extract includes the following steps: (1) crushing Senna obtusifolia seeds into a Senna obtusifolia seed powder; (2) extracting the Senna obtusifolia seed powder with 40-85% ethanol, filtering to obtain an extract solution and a residue; (3) concentrating the extract solution under vacuum to obtain a concentrated extract solution, spray-drying the concentrated extract solution to obtain the Senna obtusifolia seed extract; (4) extracting the residue with membrane filtered water, conducting a centrifugation to obtain a supernatant; (5) adding ammonium sulfate and ethanol to the supernatant to form a two-phase aqueous system, collecting a bottom layer of the two-phase aqueous system; and (6) conducting an ultrafiltration of the bottom layer with a cut-off molecular weight of 50 k-200 k to obtain a galactomannan extract solution, drying the galactomannan extract solution under vacuum to obtain the galactomannan extract.