Anti-microbial metabolites secreted from various bacterial and/or fungal species are described that have anti-bacterial activity against Xanthomonas species, which cause disease in plant hosts, including a wide variety of crops. Bacterial and fungal species producing such metabolites were identified from environmental samples (seeds, different vegetable organs and soil) from different geographic locations. Compositions, methods, uses, and kits relating to the identified anti-microbial metabolites are also described.

The present invention provides a human-derived molecularly modified insecticidal protein, and a preparation method and application thereof, and belongs to the field of genetic engineering and biological control. The present invention provides a human-derived molecularly modified insecticidal protein, and the amino acid sequence of the insecticidal protein CCL-CCL_scFv is shown as SEQ ID No. 1. The insecticidal protein CCL-CCL_scFv shows significantly higher affinity with midgut BBMV of Plutella xylostella than Cry1Ab toxin, competes with Cry1Ab and Cry1Ac toxins for binding the midgut BBMV of Plutella xylostella, and is a mimic of Cry1Ab and Cry1Ac toxins. Through Plutella xylostella indoor insecticidal biological activity assay, the insecticidal protein shows a good insecticidal effect, and can effectively replace Cry1Ac or Cry1Ab toxin for biological control of insect pest.

The present invention provides a human-derived molecularly modified insecticidal protein, and a preparation method and application thereof, and belongs to the field of genetic engineering and biological control. The present invention provides a human-derived molecularly modified insecticidal protein, and the amino acid sequence of the insecticidal protein CCL-CCL_scFv is shown as SEQ ID No. 1. The insecticidal protein CCL-CCL_scFv shows significantly higher affinity with midgut BBMV of Plutella xylostella than Cry1Ab toxin, competes with Cry1Ab and Cry1Ac toxins for binding the midgut BBMV of Plutella xylostella, and is a mimic of Cry1Ab and Cry1Ac toxins. Through Plutella xylostella indoor insecticidal biological activity assay, the insecticidal protein shows a good insecticidal effect, and can effectively replace Cry1Ac or Cry1Ab toxin for biological control of insect pest.

The present invention provides a human-derived molecularly modified insecticidal protein, and a preparation method and application thereof, and belongs to the field of genetic engineering and biological control. The present invention provides a human-derived molecularly modified insecticidal protein, and the amino acid sequence of the insecticidal protein CCL-CCL_scFv is shown as SEQ ID No. 1. The insecticidal protein CCL-CCL_scFv shows significantly higher affinity with midgut BBMV of Plutella xylostella than Cry1Ab toxin, competes with Cry1Ab and Cry1Ac toxins for binding the midgut BBMV of Plutella xylostella, and is a mimic of Cry1Ab and Cry1Ac toxins. Through Plutella xylostella indoor insecticidal biological activity assay, the insecticidal protein shows a good insecticidal effect, and can effectively replace Cry1Ac or Cry1Ab toxin for biological control of insect pest.

The present invention relates to a composition comprising at least one polypeptide, which polypeptide comprises the amino acid sequence set out in any one of SEQ ID NOs: 1 to 51 or 101 to 111 or an amino acid sequence having at least about 80% sequence identify thereto and which polypeptide is capable of binding to a fungus. The invention further relates to a composition comprising at least one polypeptide, which polypeptide comprises a CDR1 region having the amino acid sequence set out in any one of SEQ ID NOs: 52 to 67 or 112 to 122, a CDR2 region having the amino acid sequence set out in any one of SEQ ID NOs: 68 to 83 or 123 to 133, and a CDR3 region having the amino acid sequence set out in any one of SEQ ID NOs: 84 to 100 or 134 to 144 and which polypeptide is capable of binding to a fungus. The compositions may be used as anti-fungal compositions.

The present invention relates to a composition comprising at least one polypeptide, which polypeptide comprises the amino acid sequence set out in any one of SEQ ID NOs: 1 to 51 or 101 to 111 or an amino acid sequence having at least about 80% sequence identify thereto and which polypeptide is capable of binding to a fungus. The invention further relates to a composition comprising at least one polypeptide, which polypeptide comprises a CDR1 region having the amino acid sequence set out in any one of SEQ ID NOs: 52 to 67 or 112 to 122, a CDR2 region having the amino acid sequence set out in any one of SEQ ID NOs: 68 to 83 or 123 to 133, and a CDR3 region having the amino acid sequence set out in any one of SEQ ID NOs: 84 to 100 or 134 to 144 and which polypeptide is capable of binding to a fungus. The compositions may be used as anti-fungal compositions.

The disclosure provides nucleic acids, and variants and fragments thereof, derived from strains of Bacillus thuringiensis encoding variant polypeptides having increased pesticidal activity against insect pests, including Lepidoptera and Coleopteran. Particular embodiments of the disclosure provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The disclosure provides nucleic acids, and variants and fragments thereof, derived from strains of Bacillus thuringiensis encoding variant polypeptides having increased pesticidal activity against insect pests, including Lepidoptera and Coleopteran. Particular embodiments of the disclosure provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

Compositions and methods related to plant root protection, biological stimulation, and plant biomass enhancement are described. The compositions may comprise a dry blend of a low methoxyl (LM)-pectin powder; calcium and a sugar blend and are characterized by a viscosity, surface tension and saccharide composition comparable to plant mucilage and/or extracellular polymeric substances (EPS).

Compositions and methods related to plant root protection, biological stimulation, and plant biomass enhancement are described. The compositions may comprise a dry blend of a low methoxyl (LM)-pectin powder; calcium and a sugar blend and are characterized by a viscosity, surface tension and saccharide composition comparable to plant mucilage and/or extracellular polymeric substances (EPS).