The instant disclosure provides (among other things) improved methods of preparing fluorenyl-based polymeric reagents, methods of recovering and purifying such polymeric reagents, methods of reducing unwanted impurities in a fluorenyl-based polymeric reagent, fluorenyl-based polymeric reagents prepared by the methods described herein, and conjugates prepared by reaction with fluorenyl-based polymeric reagents prepared by the methods described herein.

The instant disclosure provides (among other things) improved methods of preparing fluorenyl-based polymeric reagents, methods of recovering and purifying such polymeric reagents, methods of reducing unwanted impurities in a fluorenyl-based polymeric reagent, fluorenyl-based polymeric reagents prepared by the methods described herein, and conjugates prepared by reaction with fluorenyl-based polymeric reagents prepared by the methods described herein.

Provided herein are methods of preparing a poloxamer for use in a cell culture medium. Also provided herein are cell culture media containing the poloxamer prepared by the methods herein, as well as methods of using the media for cell culturing and polypeptide production from cells.

Provided herein are methods of preparing a poloxamer for use in a cell culture medium. Also provided herein are cell culture media containing the poloxamer prepared by the methods herein, as well as methods of using the media for cell culturing and polypeptide production from cells.

The present disclosure relates to the technical field of the production of polyether polyols, and provided is a process for producing a low-odor polyether polyol, the process comprising the following steps: an initial polymerization reaction step, involving: adding an initiator and an alkaline catalyst into a reaction container, and then inputting an epoxy olefin into the reaction container for a polymerization reaction to obtain a mixed material; a circulation distribution polymerization step, involving: taking the mixed material, outputting same, splitting same and then spraying same into the reaction container at a high speed, circulating the above operations while inputting the epoxy olefin and maintaining a rotation speed of 90-105 r/min for stirring the mixed material that has been sprayed into the reaction container, continuing to proceed with the polymerization reaction, and curing same to obtain a crude polyether polyol; and a refining step, involving: taking the crude polyether polyol for a neutralization or dilution treatment to obtain a mixed solution of the crude polyether polyol, then aggregating a mixed solution stream by means of a hydrophilic medium, settling and separating to obtain the low-odor polyether polyol. The production process of the present disclosure has the advantages of a short processing time, a high yield and a low VOC content.

The present disclosure relates to the technical field of the production of polyether polyols, and provided is a process for producing a low-odor polyether polyol, the process comprising the following steps: an initial polymerization reaction step, involving: adding an initiator and an alkaline catalyst into a reaction container, and then inputting an epoxy olefin into the reaction container for a polymerization reaction to obtain a mixed material; a circulation distribution polymerization step, involving: taking the mixed material, outputting same, splitting same and then spraying same into the reaction container at a high speed, circulating the above operations while inputting the epoxy olefin and maintaining a rotation speed of 90-105 r/min for stirring the mixed material that has been sprayed into the reaction container, continuing to proceed with the polymerization reaction, and curing same to obtain a crude polyether polyol; and a refining step, involving: taking the crude polyether polyol for a neutralization or dilution treatment to obtain a mixed solution of the crude polyether polyol, then aggregating a mixed solution stream by means of a hydrophilic medium, settling and separating to obtain the low-odor polyether polyol. The production process of the present disclosure has the advantages of a short processing time, a high yield and a low VOC content.

The present invention provides a method for producing a hetero-type monodisperse polyethylene glycol, which includes a step (A) of carrying out a nucleophilic substitution reaction between a compound of the formula (2) and a compound of the formula (3) so as to satisfy the requirement of the expression (F1) to obtain a compound of the formula (4), a step (B) of carrying out the Michaels addition reaction of a compound of the formula (5) to the compound of the formula (4) at a temperature condition of 10° C. or lower to obtain a compound of the formula (6), a step (C) of detritylating or debenzylating the compound of the formula (6) to obtain a reaction product containing a compound of the formula (7), a step (D) of purifying the compound of formula (7) from the reaction product, a step (E) of reacting the compound of the formula (7) with phthalimide and performing dephthalimidation to obtain a compound of the formula (8), and a step (F) of subjecting the compound of formula (8) to an acid hydrolysis treatment to obtain a compound represented by the formula (1).

The present invention provides a method for producing a hetero-type monodisperse polyethylene glycol, which includes a step (A) of carrying out a nucleophilic substitution reaction between a compound of the formula (2) and a compound of the formula (3) so as to satisfy the requirement of the expression (F1) to obtain a compound of the formula (4), a step (B) of carrying out the Michaels addition reaction of a compound of the formula (5) to the compound of the formula (4) at a temperature condition of 10° C. or lower to obtain a compound of the formula (6), a step (C) of detritylating or debenzylating the compound of the formula (6) to obtain a reaction product containing a compound of the formula (7), a step (D) of purifying the compound of formula (7) from the reaction product, a step (E) of reacting the compound of the formula (7) with phthalimide and performing dephthalimidation to obtain a compound of the formula (8), and a step (F) of subjecting the compound of formula (8) to an acid hydrolysis treatment to obtain a compound represented by the formula (1).

The present invention relates to a process for providing a homogeneous polyether polyol composition comprising the following steps: Providing a crude polyether polyol mixture comprising polyether polyol and a Group IA or Group IIA metal ion; Neutralisation of said crude polyether polyol mixture by adding a strong acid to said mixture, resulting in a neutralised polyether polyol composition, which comprises said polyether polyol along with a salt of said Group IA or Group IIA metal ion and said strong acid; Providing said homogeneous polyether polyol composition. characterised in that said strong acid is a pentavalent organic phosphorus compound, and in that said salt is a phosphorus-containing compound based organic salt, which is soluble in said polyether polyol composition.

A method for separating a double metal cyanide catalyst (DMC catalyst) from polyol, comprising: A) initially charging a polyol comprising DMC catalyst, an alcohol, and optionally a filtration aid into a reactor, the mixture being heated, B) filtering the mixture from step A), and C) optionally separating the alcohol from the filtrate of step B), wherein in step A), as the alcohol, 4% by weight to 12% by weight of ethanol, based on 100% by weight of polyol, without a chelating agent is used.