Methods and compositions for making bacteriocins are described in some embodiments herein. In some embodiments, pro-polypeptide comprising the bacteriocins in the desired ratios in cis, and separated by cleavage sited can be produced by a microbial cell comprising a nucleic acid encoding the pro-polypeptide. In some embodiments microfluidic devices and methods for making specified mixtures of antimicrobial peptides and/or bacteriocins are described.

Methods and compositions for making bacteriocins are described in some embodiments herein. In some embodiments, pro-polypeptide comprising the bacteriocins in the desired ratios in cis, and separated by cleavage sited can be produced by a microbial cell comprising a nucleic acid encoding the pro-polypeptide. In some embodiments microfluidic devices and methods for making specified mixtures of antimicrobial peptides and/or bacteriocins are described.

A protein product capable of immobilizing a target and being cleared as required includes a protein (lysozyme, bovine serum albumin (BSA), whey albumin, insulin, α-lactalbumin, fibrinogen, ribonuclease A, cytochrome c, α-amylase, horseradish peroxidase (HRP), pepsin, myoglobin, collagen, keratin, or the like), a protein modifier (a strong oxidizing agent or a strong reducing agent), and a matter to be immobilized (a coating, a pesticide, a chemical fertilizer, a cosmetic, a medical drug, or the like). A protein film can be generated through the phase transition of the protein to immobilize the matter to be immobilized on a target surface, thereby improving the utilization rate of the matter to be immobilized and reducing the resource waste and environmental pollution caused by the loss of the matter to be immobilized. In addition, after the protein film is decomposed, the matter to be immobilized will also be effectively cleared.

A protein product capable of immobilizing a target and being cleared as required includes a protein (lysozyme, bovine serum albumin (BSA), whey albumin, insulin, α-lactalbumin, fibrinogen, ribonuclease A, cytochrome c, α-amylase, horseradish peroxidase (HRP), pepsin, myoglobin, collagen, keratin, or the like), a protein modifier (a strong oxidizing agent or a strong reducing agent), and a matter to be immobilized (a coating, a pesticide, a chemical fertilizer, a cosmetic, a medical drug, or the like). A protein film can be generated through the phase transition of the protein to immobilize the matter to be immobilized on a target surface, thereby improving the utilization rate of the matter to be immobilized and reducing the resource waste and environmental pollution caused by the loss of the matter to be immobilized. In addition, after the protein film is decomposed, the matter to be immobilized will also be effectively cleared.

The invention relates to methods, uses, systems, arrays, engineered nucleotide sequences and vectors for inhibiting bacterial population growth or for altering the relative ratio of sub-populations of first and second bacteria in a mixed population of bacteria. The invention is particularly useful, for example, for treatment of microbes such as for environmental, medical, food and beverage use. The invention relates inter alia to methods of controlling microbiologically influenced corrosion (MIC) or biofouling of a substrate or fluid in an industrial or domestic system.

The invention relates to methods, uses, systems, arrays, engineered nucleotide sequences and vectors for inhibiting bacterial population growth or for altering the relative ratio of sub-populations of first and second bacteria in a mixed population of bacteria. The invention is particularly useful, for example, for treatment of microbes such as for environmental, medical, food and beverage use. The invention relates inter alia to methods of controlling microbiologically influenced corrosion (MIC) or biofouling of a substrate or fluid in an industrial or domestic system.

The invention relates to methods, uses, systems, arrays, engineered nucleotide sequences and vectors for inhibiting bacterial population growth or for altering the relative ratio of sub-populations of first and second bacteria in a mixed population of bacteria. The invention is particularly useful, for example, for treatment of microbes such as for environmental, medical, food and beverage use. The invention relates inter alia to methods of controlling microbiologically influenced corrosion (MIC) or biofouling of a substrate or fluid in an industrial or domestic system.

The subject invention relates in part to the surprising discovery that Cry1Da is active against corn earworm (CEW), Helicoverpa zea (Boddie). Methods for using Cry1Da in transgenic plants to prevent serious crop damage is described. Leaf and silk bioassays using transgenic maize expressing full length, core toxin region or chimeric Cry1Da demonstrated good insect protection against CEW larvae damage.

The subject invention relates in part to the surprising discovery that Cry1Da is active against corn earworm (CEW), Helicoverpa zea (Boddie). Methods for using Cry1Da in transgenic plants to prevent serious crop damage is described. Leaf and silk bioassays using transgenic maize expressing full length, core toxin region or chimeric Cry1Da demonstrated good insect protection against CEW larvae damage.

The subject invention relates in part to the surprising discovery that Cry1Da is active against corn earworm (CEW), Helicoverpa zea (Boddie). Methods for using Cry1Da in transgenic plants to prevent serious crop damage is described. Leaf and silk bioassays using transgenic maize expressing full length, core toxin region or chimeric Cry1Da demonstrated good insect protection against CEW larvae damage.