Nucleotide sequences are disclosed encoding novel, insecticidal TIC4747 and related proteins exhibiting Hemipteran and Lepidopteran inhibitory activity, as well as fragments thereof. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing a polynucleotide construct encoding one or more of the toxin proteins within the TIC4747-related protein toxin class.

Nucleotide sequences are disclosed encoding novel, insecticidal TIC4747 and related proteins exhibiting Hemipteran and Lepidopteran inhibitory activity, as well as fragments thereof. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing a polynucleotide construct encoding one or more of the toxin proteins within the TIC4747-related protein toxin class.

The present invention provides a novel class of insecticides for control of disease vector insects, particularly mosquitoes. These insecticides prevent maturation or development of larvae into adult insects using interfering RNA (iRNA). The present invention further includes compositions comprising iRNA and methods of controlling, reducing, or treating an insect infestation with the iRNA or compositions described herein.

The present invention provides a novel class of insecticides for control of disease vector insects, particularly mosquitoes. These insecticides prevent maturation or development of larvae into adult insects using interfering RNA (iRNA). The present invention further includes compositions comprising iRNA and methods of controlling, reducing, or treating an insect infestation with the iRNA or compositions described herein.

The present disclosure describes a DNA fragment mixture for the prevention or for the treatment of at least one pathogenic, parasitic or infesting species of plants or of the environment, wherein the DNA mixture consists of random fragments of total DNA of at least one pathogenic, parasitic, or infesting species, and/or at least one phylogenetically similar species, against which the prevention and treatment are directed. Further, the disclosure describes a process and related system for improvement of the production/growth of microorganisms at high yield in bioreactors or photobioreactors, or of plants in different culture systems, where the nucleic acids of the organisms produced/grown by such a process are removed from the culture medium and the culture medium, deprived of these nucleic acid, is used again in the process.

The present disclosure describes a DNA fragment mixture for the prevention or for the treatment of at least one pathogenic, parasitic or infesting species of plants or of the environment, wherein the DNA mixture consists of random fragments of total DNA of at least one pathogenic, parasitic, or infesting species, and/or at least one phylogenetically similar species, against which the prevention and treatment are directed. Further, the disclosure describes a process and related system for improvement of the production/growth of microorganisms at high yield in bioreactors or photobioreactors, or of plants in different culture systems, where the nucleic acids of the organisms produced/grown by such a process are removed from the culture medium and the culture medium, deprived of these nucleic acid, is used again in the process.

Herein is disclosed synthetic oligonucleotides comprising 2′F-ANA nucleosides that can be utilized to control plant-chewing and phloem-feeding insects, bacteria present in such insects, and bacteria present in plants. The novel approaches and materials provided herein allow for reduction of pesticide and antibiotic use without the need to create genetically modified plants.

Methods and compositions are provided which employ a silencing element that, when ingested by a plant insect pest, such as a Coleopteran plant pest or a Diabrotica plant pest, decrease the expression of a target sequence in the pest. Disclosed are various target polynucleotides set forth in any one of SEQ ID NOS: 1-33 disclosed herein, (but not including the forward and reverse primers.) or variants and fragments thereof, or complements thereof, wherein a decrease in expression of one or more of the sequences in the target pest controls the pest (i.e., has insecticidal activity). Plants, plant parts, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

Methods and materials for improved delivery of dsRNA are presented. In particular, methods for controlling an insect pest by stably delivering large quantities of dsRNA to the insect pest are provided. Compositions comprising a bacterium containing a large quantity of dsRNA targeting an insect gene in which the dsRNA exhibits increased stability and activity are also provided.

Methods and materials for improved delivery of dsRNA are presented. In particular, methods for controlling an insect pest by stably delivering large quantities of dsRNA to the insect pest are provided. Compositions comprising a bacterium containing a large quantity of dsRNA targeting an insect gene in which the dsRNA exhibits increased stability and activity are also provided.