A bioprintable material is provided. The bioprintable material includes a hydrogel and microfilaments mixed in the hydrogel. The hydrogel includes a first collagen. The microfilament includes a second collagen. The diameter of the microfilament is ranging from 5 microns to 200 microns. The weight ratio of the microfilaments to the first collagen is ranging from 0.01:1 to 10:1.

Various embodiments are described herein for the fabrication enzyme degradable hydrogels useful as payload delivery systems. More particularly, embodiments disclosed herein relate to enzyme-degradable hydrogel systems comprising a crosslinkable polymer, such as a chemically-modified biopolymer, for example, chemically-modified gelatin, the hydrogel formed by a method comprising sequential physical and chemical crosslinking steps, for delivery of various payloads. Enzymes may be selected and administered to tune the release profile of the hydrogel. The payload can be, but not limited to, drugs, markers, cells, or these members encapsulated within another drug delivery such as a nanoparticle, or liposome. The hydrogel system can also be combined with another device such as a contact lens or bandage for wound healing.

The invention relates to a new protein hydrogel created on the basis of low-concentrated components: reagents A and B, the method of hydrogel preparation and its use.

Provided are biomaterials useful for cell culture, method of preparation thereof, and use thereof. The present biomaterial comprises a crosslinked hydrogel and a peptide chemically attached to the hydrogel, wherein the peptide comprises a histidine-alanine-valine (HAV) sequence. In particular, the present biomaterial may be useful for culturing neurons, brain endothelial cells, and/or glial cells, supporting the formation of synaptically connected neural networks, and growing stem cell-derived organoids that more closely resemble human organs.

Disclosed herein are gelatin particles including gelatin, wherein when a major-axis length of dried gelatin particles is defined as a and a major-axis length of gelatin particles after swelling treatment obtained by immersing the dried gelatin particles in water at 40° C. under an atmospheric pressure for 60 minutes is defined as b, swelling degree represented by b/a is 1.0 or more but 10.0 or less, and wherein the gelatin particles after swelling treatment have a particle diameter of 1.0 nm or more but 5.0 μm or less. The gelatin particles are easily taken up by cells themselves.

A preparation method of calcium peroxide-mediated in situ crosslinkable hydrogel as a sustained oxygen-generating matrix, includes: a) reacting a natural or a synthetic polymer with Traut's reagent (TR) in a solvent, and synthesizing a polymer derivative having thiol group in backbone of the polymer derivative; and b) mixing and reacting a solution of the polymer derivative having thiol group with calcium peroxide (CaO.sub.2), and thereby forming a hydrogel, wherein in the step b), disulfide bonds (—S—S) are induced between backbones of the polymer derivative having thiol group attached by decomposition of calcium peroxide (CaO.sub.2), and thereby in situ crosslinking is formed.

A method of bonding together surfaces of two or more elements. The method includes the steps of providing two or more elements, applying an adhesive to one or more of the surfaces to be bonded together before, during or after contacting the surfaces to be bonded together with each other, and curing the adhesive, wherein the adhesive comprises at least one hydrocolloid.

The present invention provides compositions and methods for repair and reconstruction of defects and injuries to soft tissues. Some aspects of the invention provide tissue adhesives comprising a hybrid hydrogel by using a naturally derived polymer, gelatin and a synthetic polymer, polyethylene glycol, wherein the hydrogel is biocompatible, biodegradable, transparent, strongly adhesive to corneal tissue, and have a smooth surface and biomechanical properties similar to the cornea.

The present invention provides a nanocomposite hydrogel and a preparation method thereof, and relates to the field of nanocomposite materials. The nanocomposite hydrogel is prepared by mixing completely gelatinized short amylose with an aqueous gelatin solution having a mass concentration of 8%-14%, and then cooling. The present invention utilizes the nanoparticles formed by in-situ self-assembly of the short amylose in the aqueous gelatin solution as a reinforcing agent, and the nanoparticles are uniformly distributed in the hydrogel to form a stable crystallization system, such that the prepared nanocomposite hydrogel exhibits optimal mechanical properties in terms of viscoelasticity, hardness, compressive stress, etc. The preparation process of the present invention is green and environmentally friendly, simple and efficient, and can be widely applied to the fields of food, cosmetics and medicine.

Disclosed is a preparation method of a dual-network collagen-based supramolecular hydrogel, including: preparing a silicate nanosheet dispersion and a collagen solution separately; mixing the silicate nanosheet dispersion with the collagen solution followed by stirring to obtain a collagen-silicate nanosheet network; preparing a tannic acid solution and a polyethylene glycol solution separately; mixing the tannic acid solution with the polyethylene glycol solution to obtain a polyethylene glycol-tannic acid network; and subjecting the collagen-silicate nanosheet network and the polyethylene glycol-tannic acid network to reaction to obtain the dual-network collagen-based supramolecular hydrogel. A dual-network collagen-based supramolecular hydrogel prepared by such method is also provided.