Protected fluorescent reagent compounds and their methods of synthesis are provided. The compounds are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The compounds contain fluorescent dye elements, that allow the compounds to be detected with high sensitivity at desirable wavelengths, binding elements, that allow the compounds to be recognized specifically by target biomolecules, and protective shield elements, that decrease undesirable contacts between the fluorescent dye elements and the bound target biomolecules and that therefore decrease photodamage of the bound target biomolecules by the fluorescent dye elements.

The present invention provides methods, compositions, and kits useful in preparing labeled molecules, which are useful in the detection of binding partners.

The present invention provides methods, compositions, and kits useful in preparing labeled molecules, which are useful in the detection of binding partners.

Provided are any one of the following fluorescent compounds and a fluorescent labeled biological substance having this fluorescent compound.

##STR00001##

X represents CR.sup.5 or N, and R.sup.1 to R.sup.7, Q.sup.1, Q.sup.2, L.sup.1, and L.sup.2 each represent a specific group.

a ring .sub.1 and a ring .sub.2 are a 5- to 8-membered ring,

At least one of R.sup.1 to R.sup.7, L.sup.1, L.sup.2, Q.sup.1, or Q.sup.2 has a specific hydrophilic group.

However, in a case where the ring .sub.1 and the ring .sub.2 are a 6-membered ring, and L.sup.1 and L.sup.2 are an arylene group, R.sup.5 is not an aryl group substituted with a linear alkyl group having 18 or more carbon atoms. In addition to the regulation of the ring .sub.1, the ring .sub.2, L.sup.1, and L.sup.2, in a case where R.sup.5 has a substituent having a dipyrromethene boron complex structure, the dipyrromethene boron complex structure has a structure in which a dipyrromethene skeleton is coordinately bonded to a boron atom in the tridentate or tetradentate coordination.

Provided are any one of the following fluorescent compounds and a fluorescent labeled biological substance having this fluorescent compound.

##STR00001##

X represents CR.sup.5 or N, and R.sup.1 to R.sup.7, Q.sup.1, Q.sup.2, L.sup.1, and L.sup.2 each represent a specific group.

a ring .sub.1 and a ring .sub.2 are a 5- to 8-membered ring,

At least one of R.sup.1 to R.sup.7, L.sup.1, L.sup.2, Q.sup.1, or Q.sup.2 has a specific hydrophilic group.

However, in a case where the ring .sub.1 and the ring .sub.2 are a 6-membered ring, and L.sup.1 and L.sup.2 are an arylene group, R.sup.5 is not an aryl group substituted with a linear alkyl group having 18 or more carbon atoms. In addition to the regulation of the ring .sub.1, the ring .sub.2, L.sup.1, and L.sup.2, in a case where R.sup.5 has a substituent having a dipyrromethene boron complex structure, the dipyrromethene boron complex structure has a structure in which a dipyrromethene skeleton is coordinately bonded to a boron atom in the tridentate or tetradentate coordination.

Provided are dyes and compositions which are useful in a number of applications, such as the detection and monitoring protein aggregation, kinetic studies of protein aggregation, neurofibrillary plaques analysis, evaluation of protein formulation stability, and analysis of molecular chaperone activity.

Provided are dyes and compositions which are useful in a number of applications, such as the detection and monitoring protein aggregation, kinetic studies of protein aggregation, neurofibrillary plaques analysis, evaluation of protein formulation stability, and analysis of molecular chaperone activity.

Provided are a dispersion, applications thereof, and a compound, the dispersion including: a colorant represented by the following Formula 1; and a medium. In Formula 1, L.sup.1 represents a methine chain consisting of 5 or 7 methine groups, a methine group at a center of the methine chain has a substituent represented by the following Formula A, R.sup.1, R.sup.2, R.sup.3, and R.sup.4 each independently represent a hydrogen atom, an alkyl group, an aryl group, or a monovalent heterocyclic group, and X's each independently represent an oxygen atom, a sulfur atom, or a selenium atom. In Formula A, SA represents a single bond, an alkylene group, an alkenylene group, or an alkynylene group, T.sup.A represents a hydrogen atom, a halogen atom, an alkyl group, or an aryl group, and * represents a binding site to the methine group at the center of the methine chain.

The present invention provides methods, compositions, and kits useful in preparing labeled molecules, which are useful in the detection of binding partners.

The present invention provides methods, compositions, and kits useful in preparing labeled molecules, which are useful in the detection of binding partners.