The present disclosure provides stabilized hematoxylin formulations having a pH of less than 2.4. In general, the stabilized hematoxylin formulations comprise a solvent, a hematoxylin dye, and an acid. The present disclosure also provides methods of using such stabilized hematoxylin formulations to stain biological samples. The present disclosure also provides for methods of readjusting the pH of a stabilized hematoxylin formulation to provide a hematoxylin staining solution.

The present disclosure provides stabilized hematoxylin formulations having a pH of less than 2.4. The present disclosure also provides methods of using such stabilized hematoxylin formulations to stain biological samples.