The invention provides a disperse dye color paste for waterless dyeing, which comprises, by weight, 10%-40% of disperse dye filter cakes, 2%-35% of a dispersant, 5%-20% of a dyeing auxiliary, 0.1-0.5% of a surfactant, and 20%-88% of a solvent. The disperse dye color paste for waterless dyeing of the invention has a small particle size; and after waterless dyeing, a textile does not contain cleavable carcinogenic aromatic amine azo dyes, allergenic dyes, forbidden aromatic amine or bioactive substances. The disperse dye color paste for waterless dyeing of the invention can be stably stored for 6-12 months which is beneficial to the transportation of dyes and the production management of dyeing factories. Moreover, a dyeing effect featuring a high fixation rate is realized after the disperse dye color paste for waterless dyeing is applied to textiles.

The disclosure relates to an improved Oil Red O staining solution, which consists of 0.5% of Oil Red O, 50% of ethanol, and 5% to 10% of salicylic acid. The staining solution does not contain organic solvents toxic to human bodies and has good safety and no toxic effect. The disclosure further relates to an Oil Red O staining method for cells or tissues, which is simple in operation, short in staining time, and easy to stain neutral fat in cells or tissues, with good staining effect and clean background. This method does not use isopropanol, which is safe and convenient and is suitable for popularization and application in laboratories.

The present invention relates to a method of preparing a colored gloss pigment using a natural dye. According to the present disclosure, the method may include preparing a dye from a dye material of natural dyes; titrating the dye with an inorganic salt solution and subsequently adjusting a pH neutral to prepare a natural pigment containing the dye; dispersing a substrate coated with a metal oxide into a DI water to form a substrate suspension; and introducing a pH adjusting agent and the natural pigment into the substrate suspension to coat a surface of the substrate with the natural pigment.